Genetic and Biochemical Diversity of Clinical <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i> Isolates in a Public Hospital in Brazil

Santos, Milena Danda Vasconcelos; Barros, Maria Paloma Silva de; Silveira-Filho, Vladimir da Mota; Mendes-Marques, Carina Lucena; Lima, Adilson Celestino de; Silva, Márcia Vanusa da; Leal-Balbino, Tereza Cristina; Silva, María da Paz Carvalho da; Paiva, Patrícia Maria Guedes; Oliveira, Maria Betânia Melo de

doi:10.1089/mdr.2020.0154

Cited by 4 publications

(1 citation statement)

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“…Effective discrimination of different P. aeruginosa genotypes using MLVA has been reported previously [13]. Since most of the loci on the agarose gel are determined by polymerase chain reaction (PCR), this technique is considered to be repeatable, economical and least time consuming [14]. The popularity of MLVA as a clonality genotyping method for hospital-based epidemiological surveys has increased in recent years [12].…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance, biofilm production ability and genetic diversity of carbapenem-resistant Pseudomonas aeruginosa strains isolated from nosocomial infections in southwestern Iran

et al. 2022

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Background This study was aimed to evaluate the antibiotic resistance, biofilm formation, and genetic diversity of carbapenem-resistant Pseudomonas aeruginosa (CRPA) strains isolated from four types of nosocomial infections (NIs) including urinary tract infection (UTI), ventilator-associated pneumonia (VAP), surgical site infection (SSI), and bloodstream infection (BSI). Methods and resultsIn total, 115 isolates of NIs-causing P. aeruginosa were collected from NIs. Antibiotic susceptibility testing (AST) was performed using disk diffusion method and minimum inhibitory concentrations. Biofilm formation was tested on 96-well polystyrene microtiter plates (MTP). CRPA isolates were genotyped using multiple-locus variable number of tandem repeat analysis (MLVA). The most resistance and susceptibility rates were observed to amikacin (70.6%) and colistin (96.1%), respectively. Colistin and meropenem were the most active antimicrobial agents in VAP, SSI, and BSI. While, colistin and cefepime were the most active in UTIs. In total, 52.2% (n = 60/115) of P. aeruginosa isolates were carbapenem resistant, of which 95.0%, 55.0%, and 5.0% were multidrug-resistant, extensively drug-resistant, and pandrug-resistant, respectively. There was a significant association between resistance to carbapenem and resistance to other antibiotics except for piperacillin/tazobactam. The biofilm production of CRPA isolates was 95.0%, of which 23.3% were strong biofilm producers. Based on MLVA, there were 34 different types of CRPA isolates classified into three main clusters and 5 sub clusters. ConclusionThe association of CRPA with other antibiotic resistance, the high rates of biofilm production, and the high genetic diversity of the isolates may be a warning of the need for a careful surveillance program.

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Section: Introductionmentioning

confidence: 99%