Genetic and Biochemical Diversity of Hypericum Perforatum L. Grown in the Caspian Climate of Iran

Abstract

Rahnavrd: Genetic and biochemical diversity of Hypericum perforatum L.- Abstract. Hypericum perforatum, also known as St John's wort, is an important medicinal plant that produces many secondary metabolites that have anti-viral, anti-bacterial, and anti-depression properties.The most effective drug substance in this plant is hypericin that changed in the various genotypes. To investigate the genetic and biochemical diversity of Hypericum perforatum, samples were collected from different genotypes around the Caspian climate region. A total of 15 genotypes from the Hyrcanian Province in the Caspian climate region were evaluated using 15 randomized amplified primers design (RAPD primers). A total number of 90 bands with the average of bands amplified by each primer was 9-band. The number of polymorphic bands per primer ranged from 1 to 13 and the bands were 250 to 3000 bp in size. Based on the results, OPAD-10 primer with 13 bands and OPV10 with 7 bands were used as the maximum and minimum number of amplified fragment, respectively. Molecular marker genotypes showed a high degree of polymorphism. Based on the RAPD results, the genotypes were divided into 4 groups. Most similar genotypes with a coefficient of 75% were in subgroup A 3 ( Noshahr and Kelardasht ) . Variation in hypericin was very significant in the genotypes grown under identical conditions. The highest amount of hypericin was measured in the Kelardasht genotype and the lowest amount in the Roodsar genotype.

Investigation of gene expression diversity in Hypericum spp. before and after flowering under different nitrogen fertilization levels

“…Regarding the photodynamic properties of hypericin, this metabolite has therapeutic potential as an antiviral, antiretroviral, and anticancer agent (Zhang et al, 2016, Jendželovská et al, 2016Kubin et al, 2005). Hypericin, a valuable polycyclic dianthroquinone, is responsible for the antidepression and antiviral characteristics (Rahnavrd, 2017).…”

“…After several steps emodin is made, phenolic oxidative coupling protein (Hyp-1) catalyzes the emodin anthrone production from emodin, then Hyp-1 catalyzes the emodin anthrone conversion to protohypericin, and finally, Hyp-1 catalyzes the formation of hypericin from protohypericin. Therefore the genes hyp-1, HpPKS1, and HpPKS2 are playing important roles in hypericin biosynthesis (Rahnavrd, 2017).…”

Infraspecific morphological and genetic variations of Ficus carica L. from Iran

“…Administration of the ethanolic extract of the leaves remarkably ameliorated both cellular and humoral antibody response in mice (Patil et al, 2010). Rahnavard (2017) reported that few studies are available on F. carica for understanding the genome structure and its infraspecific gene-tic variation. Molecular techniques are useful tools for the investigation of genetic diversity and population structure; however, morphological and biochemical methods can be used for detecting genetic variations.…”