Genetic and biochemical interactions of yeast DNA helicases

Nickens, David G.; Bochman, Matthew L.

doi:10.1016/j.ymeth.2022.04.014

Cited by 3 publications

(2 citation statements)

References 72 publications

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“…Hrq1 alone or in the presence of catalytically inactive Pif1 acts as an activator of telomerase activity ( 6 ). Similar experiments with the Sgs1 helicase, a RecQ family enzyme like Hrq1, show that it can also act as an activator of telomerase ( 5 ).…”

Section: Introductionmentioning

confidence: 79%

Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA

Nickens,

Feng,

Shen

et al. 2024

Nucleic Acids Research

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Telomerase is the enzyme that lengthens telomeres and is tightly regulated by a variety of means to maintain genome integrity. Several DNA helicases function at telomeres, and we previously found that the Saccharomyces cerevisiae helicases Hrq1 and Pif1 directly regulate telomerase. To extend these findings, we are investigating the interplay between helicases, single-stranded DNA (ssDNA) binding proteins (ssBPs), and telomerase. The yeast ssBPs Cdc13 and RPA differentially affect Hrq1 and Pif1 helicase activity, and experiments to measure helicase disruption of Cdc13/ssDNA complexes instead revealed that Cdc13 can exchange between substrates. Although other ssBPs display dynamic binding, this was unexpected with Cdc13 due to the reported in vitro stability of the Cdc13/telomeric ssDNA complex. We found that the DNA exchange by Cdc13 occurs rapidly at physiological temperatures, requires telomeric repeat sequence DNA, and is affected by ssDNA length. Cdc13 truncations revealed that the low-affinity binding site (OB1), which is distal from the high-affinity binding site (OB3), is required for this intermolecular dynamic DNA exchange (DDE). We hypothesize that DDE by Cdc13 is the basis for how Cdc13 ‘moves’ at telomeres to alternate between modes where it regulates telomerase activity and assists in telomere replication.

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Section: Introductionmentioning

confidence: 79%

Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA

Nickens,

Feng,

Shen

et al. 2024

Nucleic Acids Research

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show abstract

“…Hrq1 alone or in the presence of catalytically inactive Pif1 acts as an activator of telomerase activity (46). Similar experiments with the Sgs1 helicase, a RecQ family enzyme like Hrq1, show that it can also act as an activator of telomerase (53).…”

Section: Introductionmentioning

confidence: 80%

Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA

Nickens,

Feng,

Shen

et al. 2023

Preprint

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Telomerase is the enzyme that lengthens telomeres and is tightly regulated by a variety of means to maintain genome integrity. Several DNA helicases function at telomeres, and we previously found that theSaccharomyces cerevisiaehelicases Hrq1 and Pif1 directly regulate telomerase. To extend these findings, we are investigating the interplay between helicases, single-stranded DNA (ssDNA) binding proteins (ssBPs), and telomerase. The yeast ssBPs Cdc13 and RPA differentially affect Hrq1 and Pif1 helicase activity, and experiments to measure helicase disruption of Cdc13/ssDNA complexes instead revealed that Cdc13 can exchange between substrates. Although other ssBPs display dynamic binding, this was unexpected with Cdc13 due to the reportedin vitrostability of the Cdc13/telomeric ssDNA complex. We found that the DNA exchange by Cdc13 occurs rapidly at physiological temperatures, requires telomeric repeat sequence DNA, and is affected by ssDNA length. Cdc13 truncations revealed that the low-affinity binding site (OB1), which is distal from the high-affinity binding site (OB3), is required for this intermolecular dynamic DNA exchange (DDE). We hypothesize that DDE by Cdc13 is the basis for how Cdc13 “moves” at telomeres to alternate between modes where it regulates telomerase activity and assists in telomere replication.

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Methods special issue: DNA and RNA helicases

Brosh

2022

Methods

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Genetic and biochemical interactions of yeast DNA helicases

Cited by 3 publications

References 72 publications

Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA

Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA

Cdc13 exhibits dynamic DNA strand exchange in the presence of telomeric DNA

Methods special issue: DNA and RNA helicases

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