Genetic and Genomic Approaches for Improvement in Mungbean (Vigna radiata L.)

Das, Alok; Singh, Pushpendra; Kushwah, Neetu; Thakur, Sunil; Rathore, Meenal; Pratap, Aditya; Singh, Narendra Pratap

doi:10.1007/978-3-030-01743-9_8

Cited by 3 publications

(3 citation statements)

References 27 publications

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“…Despite all the economic and nutritional value of mung beans, the expansion of the crop is limited by a lack of genetic resources that are important for crop adaptation (Das et al, 2018). Breeding of mung beans for wider adaptation is a priority and modern marker tools are needed for selection of new varieties (Burlyaeva et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide SNP Identification and Association Mapping for Seed Mineral Concentration in Mung Bean (Vigna radiata L.)

Islam

Limpot

et al. 2020

Front. Genet.

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Mung bean (Vigna radiata L.) quality is dependent on seed chemical composition, which in turn determines the benefits of its consumption for human health and nutrition. While mung bean is rich in a range of nutritional components, such as protein, carbohydrates and vitamins, it remains less well studied than other legume crops in terms of micronutrients. In addition, mung bean genomics and genetic resources are relatively sparse. The objectives of this research were threefold , namely: to develop a genome-wide marker system for mung bean based on genotyping by sequencing (GBS), to evaluate diversity of mung beans available to breeders in the United States and finally, to perform a genome-wide association study (GWAS) for nutrient concentrations based on a seven mineral analysis using inductively coupled plasma (ICP) spectroscopy. All parts of our research were performed with 95 cultivated mung bean genotypes chosen from the USDA core collection representing accessions from 13 countries. Overall, we identified a total of 6,486 high quality single nucleotide polymorphisms (SNPs) from the GBS dataset and found 43 marker × trait associations (MTAs) with calcium, iron, potassium, manganese, phosphorous, sulfur or zinc concentrations in mung bean grain produced in either of two consecutive years' field experiments. The MTAs were scattered across 35 genomic regions explaining on average 22% of the variation for each seed nutrient in each year. Most of the gene regions provided valuable candidate loci to use in future breeding of new varieties of mung bean and further the understanding of genetic control of nutritional properties in the crop. Other SNPs identified in this study will serve as important resources to enable marker-assisted selection (MAS) for nutritional improvement in mung bean and to analyze cultivars of mung bean.

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Section: Introductionmentioning

confidence: 99%

Genome-Wide SNP Identification and Association Mapping for Seed Mineral Concentration in Mung Bean (Vigna radiata L.)

Islam

Limpot

et al. 2020

Front. Genet.

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“…Several pre-and post-fertilization barriers encumbers the alien gene introgression and genetic improvement of legume crops. Advanced breeding and molecular techniques can be employed in distant hybridization to overcome incompatibility barriers and induce homoeologous recombination for the production of interspecific hybrids and pre-breeding lines (Pratap et al, 2018). Promising and stable pre-breeding ILs identified from the multi-environmental evaluation can be utilized in the genomic-assisted legume improvement programme to develop high-yielding, supreme quality cultivars resilient to fluctuating climates and surging insect-pests.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

“…They included moth bean, minni payaru, green gram, black gram, creole bean, rice bean, and adzuki bean. Green gram ( V. radiate (L.) R. Wilczek) and black gram ( V. mungo (L.) Hepper) are the two most important legumes, which were believed to be originated from the single ancestor ( Das et al., 2018 ). Later, it has been proved that, green gram and black gram has the independent origin from V. radiata var.…”

Section: Theory Of Gene Poolmentioning

confidence: 99%

Unlocking the hidden variation from wild repository for accelerating genetic gain in legumes

et al. 2022

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The fluctuating climates, rising human population, and deteriorating arable lands necessitate sustainable crops to fulfil global food requirements. In the countryside, legumes with intriguing but enigmatic nitrogen-fixing abilities and thriving in harsh climatic conditions promise future food security. However, breaking the yield plateau and achieving higher genetic gain are the unsolved problems of legume improvement. Present study gives emphasis on 15 important legume crops, i.e., chickpea, pigeonpea, soybean, groundnut, lentil, common bean, faba bean, cowpea, lupin, pea, green gram, back gram, horse gram, moth bean, rice bean, and some forage legumes. We have given an overview of the world and India’s area, production, and productivity trends for all legume crops from 1961 to 2020. Our review article investigates the importance of gene pools and wild relatives in broadening the genetic base of legumes through pre-breeding and alien gene introgression. We have also discussed the importance of integrating genomics, phenomics, speed breeding, genetic engineering and genome editing tools in legume improvement programmes. Overall, legume breeding may undergo a paradigm shift once genomics and conventional breeding are integrated in the near future.

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Maş fasulyesi (Vigna radiata L.) tohumlarında fasulye adi mozayik virüsü’nün serolojik ve moleküler yöntemler ile belirlenmesi

ÇULAL KILIÇ,

KARAMAN,

DEMİRKURT

et al. 2022

Akademik Ziraat Dergisi

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Bu çalışma Isparta Uygulamalı Bilimler Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü’nden temin edilen 100 adet maş fasulyesi tohumu ile yürütülmüştür. Tohum örneklerinde BCMV (Bean common mosaic virus: Fasulye Adi Mozayik Virüsü)’nün tanılanması serolojik ve moleküler yöntemler kullanılarak yapılmıştır. DAS-ELISA (Double antibody sandwich enzyme linked immunosorbent assay) sonuçlarına göre; tohum örneklerinde BCMV’nün bulunma oranı % 9 olarak belirlenmiştir. RT-PCR (Reverse Transcriptase Polimerase chain reaction) çalışmalarında BCMV ile enfekteli 9 tohum örneği kullanılmıştır. RT-PCR testinde spesifik primer çiftleri kullanılarak yaklaşık 850 bp’lik bir kısım amplifiye edilmiş ve beklenen seviyede bant elde edilmiştir. Çalışma sonucunda ülkemizde ilk kez maş fasulyesi tohumlarında BCMV’nün tanılanması yapılmıştır.

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Genetic and Genomic Approaches for Improvement in Mungbean (Vigna radiata L.)

Cited by 3 publications

References 27 publications

Genome-Wide SNP Identification and Association Mapping for Seed Mineral Concentration in Mung Bean (Vigna radiata L.)

Genome-Wide SNP Identification and Association Mapping for Seed Mineral Concentration in Mung Bean (Vigna radiata L.)

Unlocking the hidden variation from wild repository for accelerating genetic gain in legumes

Maş fasulyesi (Vigna radiata L.) tohumlarında fasulye adi mozayik virüsü’nün serolojik ve moleküler yöntemler ile belirlenmesi

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