Genetic and metal analyses of fragmented populations of <i>Betula papyrifera</i> (Marsh) in a mining reclaimed region: identification of population–diagnostic molecular marker

Theriault, Gabriel; Nkongolo, K. K.; Paul, M.

doi:10.1002/ece3.1195

Cited by 20 publications

(9 citation statements)

References 28 publications

(50 reference statements)

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“…In previous studies, Kalubi et al (2015) identified an ISSR marker that differentiated red maple (Acer rubrum) populations from metal contaminated sites and uncontaminated areas in Northern Ontario. Likewise, Theriault et al (2014) characterized two populationdiagnostic ISSR markers in B. papyrifera from Northern Ontario that showed difference between St. Charles population and other populations from the Greater Sudbury areas. This result along with the observations made in the present study suggested that B. papyrifera population from St. Charles represent a distinct population from other GSR populations which might be all from a common source.…”

Section: Molecular Analysismentioning

confidence: 99%

“…Both male and female catkins lack petals enhancing B. papyrifera pollination with B. pumila flowers within the same stand. It has been hypothesized that the exchange of genetic information between Betula papyrifera and other species within the genus Betula might be a common phenomenon in open populations (Sofiev et al, 2006;Theriault et al, 2014). This should result in characteristics that are variable within species making the identification of pure and hybrid genotypes very challenging.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Molecular Markers Differentiating Betula papyrifera and B. pumila Populations from Northern Ontario (Canada)

Moarefi¹,

Paul²,

Beckett³

et al. 2018

American Journal of Environmental Sciences

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Betula is a polyploid and highly polymorphic genus with several species known to hybridize very readily. This high level of introgression has resulted in hybrid populations where different species are sympatric. The main objectives of the present study were to identify putative hybrids between B. papyrifera and B. pumila in the Greater Sudbury Region (Northern Ontario) and to develop ISSR and RAPD markers that can differentiate B. papyrifera and B. pumila and their respective populations. This study revealed extensive introgression of B. papyrifera genes into B. pumila based on morphological characterization in the Greater Sudbury region (Northern Ontario) where these two species coexist. Genomic DNAs were extracted from all the collected genotypes from seven B. papyrifera and five B. pumila populations within the GSR. Additional samples from B. pumila from Wisconsin were also analyzed. All the DNA samples were amplified using ISSR and RAPD primers. Nospecies-diagnostic markers was identified because in part to a high level of polymorphic loci observed within and among populations. A close look of all the amplified products revealed a number of ISSR and RAPD diagnostic markers that differentiate P. papyrifera populations from different origins. Likewise, diagnostic bands distinguishing B. pumila from Wisconsin to the GSR population were identified.

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Section: Molecular Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of Molecular Markers Differentiating Betula papyrifera and B. pumila Populations from Northern Ontario (Canada)

Moarefi¹,

Paul²,

Beckett³

et al. 2018

American Journal of Environmental Sciences

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“…It has been demonstrated that white birch (Betula papyrifera), trembling aspen (Populus tremuloides), and red oak (Quercus rubra) accumulate nickel in leaves. Therefore, they are classified as nickel accumulators (Mehes-Smith & Nkongolo, 2015;Theriault, Michael, & Nkongolo, 2016a;Theriault, Nkongolo, & Michael, 2014;Theriault et al, 2013;Tran et al, 2014). Red maple (Acer rubrum) on the other hand does not accumulate nickel in its tissues (the amount of bioavailable nickel in the soil is higher than the total nickel in roots).…”

Section: Introductionmentioning

confidence: 99%

Differential levels of gene expression and molecular mechanisms between red maple (Acer rubrum) genotypes resistant and susceptible to nickel toxicity revealed by transcriptome analysis

Nkongolo

Theriault

Paul

2018

Ecology and Evolution

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Knowledge of regulation of genes associated with metal resistance in higher plants is very limited. Many plant species have developed different genetic mechanisms and metabolic pathways to cope with metal toxicity. The main objectives of this study were to 1) assess gene expression dynamics of A. rubrum in response to nickel (Ni) stress and 2) describe gene function based on ontology. Certified A. rubrum genotypes were treated with 1,600 mg of Ni per 1 Kg of soil corresponding to a soil total nickel content in a metal‐contaminated region in Ontario, Canada. Nickel resistant and susceptible genotypes were selected and used for transcriptome analysis. Overall, 223,610,443 bases were generated. Trinity reads were assembled to trinity transcripts. The transcripts were mapped to protein sequences and after quality controls and appropriate trimmings, 66,783 annotated transcripts were selected as expressed among the libraries. The study reveals that nickel treatment at a high dose of 1,600 mg/kg triggers regulation of several genes. When nickel‐resistant genotypes were compared to water controls, 6,263 genes were upregulated and 3,142 were downregulated. These values were 3,308 and 2,176, respectively, when susceptible genotypes were compared to water control. The coping mechanism of A. rubrum to Ni toxicity was elucidated. Upregulation of genes associated with transport in cytosol was prevalent in resistant genotypes compared to controls while upregulation of genes associated with translation in the ribosome was higher in susceptible genotypes when compared to water. The analysis revealed no major gene associated with Ni resistance in A. rubrum. Overall, the results of this study suggest that the genetic mechanism controlling the resistance of this species to nickel is controlled by genes with limited expression. The subtle differences between resistant and susceptible genotypes in gene regulation were detected using water‐treated genotypes as references.

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“…Despite a significant reduction in metals and sulfur dioxide emissions, copper and nickel concentrations remain high. Yet the populations of several tree species in northern Ontario proliferate despite the high amount of metals in the ecosystem (Nkongolo et al, 2013;Tran et al, 2014;Theriault et al, 2014). Species such as red oak (Quercus rubra), some birch and poplar, recolonized metal contaminated areas within the GSR (Winterhalder, 1995;Makela et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Differential Gene Transcription in Red Oak (Quercus rubra) Genotypes Resistant to Copper Toxicity

Migueal¹,

Paul²,

Djeukam³

et al. 2017

American Journal of Biochemistry and Biotechnology

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Toxicity of metals is a major abiotic stressor of plants. Copper (Cu) is one of the most abundant metals in soils from the Greater Sudbury Region (GSR) due to its mining history. Recent studies have described several transporters and chelating proteins involved in copper resistance. Quercus rubra (Red Oak) is a common species that grows in metal contaminated soils in Northern Ontario (Canada). The main objectives of this study were1) to evaluate the toxicity of copper to Q. rubrum plants and 2) assess the level of transcriptionof genes associated with copper resistance (RAN1, MT2b and MRP4). Q. rubra seedlings were grown in growth chambers and treated with copper at different doses. Total RNA was extracted from leaves and amplified by RT-qPCR. All the plants tested were resistant to Cu even at high concentrations of 1312 mg of Cu per kg of dry soil since no damage associated with Cu toxicity was observed after 14 days of treatment. RAN1 transporter and the chelating protein MT2b were significantly downregulated (p≤0.05) at high doses of 656 mg and 1312 mg of copper per kg of dry soil compared to water control. The transcription of MRP4 was significantly increased in the presence of copper at a concentration of 1312 mg/kg. No gene demonstrated differential transcription in samples treated with a low dose of 130 mg of copper/kg of dry soil that is equivalent to the bioavailable amount of copper under natural conditions in the Greater Sudbury Region.

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Genetic and metal analyses of fragmented populations of Betula papyrifera (Marsh) in a mining reclaimed region: identification of population–diagnostic molecular marker

Cited by 20 publications

References 28 publications

Identification of Molecular Markers Differentiating Betula papyrifera and B. pumila Populations from Northern Ontario (Canada)

Identification of Molecular Markers Differentiating Betula papyrifera and B. pumila Populations from Northern Ontario (Canada)

Differential levels of gene expression and molecular mechanisms between red maple (Acer rubrum) genotypes resistant and susceptible to nickel toxicity revealed by transcriptome analysis

Differential Gene Transcription in Red Oak (Quercus rubra) Genotypes Resistant to Copper Toxicity

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