Genetic and molecular analyses of the C-terminal region of the recE gene from the Rac prophage of Escherichia coli K-12 reveal the recT gene

Clark, Alvin J.; Sharma, Vineet K.; Brenowitz, S; Chu, Charles C.; Sandler, S J; Satin, L.; Templin, A.; Berger, I; Cohen, A.

doi:10.1128/jb.175.23.7673-7682.1993

Cited by 49 publications

(65 citation statements)

References 36 publications

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“…RecT is derepressed by sbcA mutations, and some recombination events occurring in recBC sbcA recA mutant cells depend on it (Clark et al, 1993;Hall and Kolodner, 1994, and references therein).…”

Section: Dsbs Trigger Rdr In Recbc Sbca Mutant Cellsmentioning

confidence: 99%

DNA replication triggered by double-stranded breaks in E. coli: Dependence on homologous recombination functions

Asai

Bates

Kogoma

1994

Cell

113

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SummaryHomologous recombination-dependent DNA replication (RDR) of a λ cos site-carrying plasmid is demonstrated in E. coli cells when the cells express λ terminase that introduces a double-stranded break into the cos site. RDR occurs in normal wild-type cells if the plasmid also contains the recombination hotspot χ. χ is dispensable when cells are induced for the SOS response or contain a recD mutation. recBC sbcA mutant cells are also capable of RDR induction. A recN mutation greatly reduces RDR in normal cells, but not in SOS-induced cells. RDR proceeds by the θ mode or rolling circle mode of DNA synthesis, yielding covalently closed circular plasmid monomers or linear plasmid multimers, respectively. Previously described inducible stable DNA replication is considered to be a special type of RDR that starts exclusively from specific sites (or/Ms) on the chromosome.

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Section: Dsbs Trigger Rdr In Recbc Sbca Mutant Cellsmentioning

confidence: 99%

DNA replication triggered by double-stranded breaks in E. coli: Dependence on homologous recombination functions

Asai

Bates

Kogoma

1994

Cell

113

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“…The Red recombination functions are encoded by three adjacent genes, gam, bet and exo, in the pL operon (1). RecET functions are encoded by two adjacent genes, recE and recT, present on the cryptic lambdoid Rac prophage found in the genome of many E. coli K-12 strains (5). A variety of studies have concluded that the Exo/Beta and RecE/RecT protein pairs are functionally equivalent although not related at the sequence level (6,7).…”

mentioning

confidence: 99%

Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages

Datta

Costantino

Zhang

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

176

233

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We report the identification and functional analysis of nine genes from Gram-positive and Gram-negative bacteria and their phages that are similar to lambda () bet or Escherichia coli recT. Beta and RecT are single-strand DNA annealing proteins, referred to here as recombinases. Each of the nine other genes when expressed in E. coli carries out oligonucleotide-mediated recombination. To our knowledge, this is the first study showing single-strand recombinase activity from diverse bacteria. Similar to bet and recT, most of these other recombinases were found to be associated with putative exonuclease genes. Beta and RecT in conjunction with their cognate exonucleases carry out recombination of linear doublestrand DNA. Among four of these foreign recombinase/exonuclease pairs tested for recombination with double-strand DNA, three had activity, albeit barely detectable. Thus, although these recombinases can function in E. coli to catalyze oligonucleotide recombination, the double-strand DNA recombination activities with their exonuclease partners were inefficient. This study also demonstrated that Gam, by inhibiting host RecBCD nuclease activity, helps to improve the efficiency of Red-mediated recombination with linear double-strand DNA, but Gam is not absolutely essential. Thus, in other bacterial species where Gam analogs have not been identified, double-strand DNA recombination may still work in the absence of a Gam-like function. We anticipate that at least some of the recombineering systems studied here will potentiate oligonucleotide and double-strand DNA-mediated recombineering in their native or related bacteria.bacteriophage lambda ͉ Beta ͉ oligonucleotide recombination ͉ RecT ͉ ssDNA annealing proteins R ecombineering is a simple and efficient way to engineer DNA molecules in vivo without the in vitro use of restriction enzymes and DNA ligase (1, 2). In Escherichia coli, it allows genetic modifications, from point mutations to substitutions and deletions. Recombineering is mediated by phage-derived proteins, either the Red proteins of phage (1-3) or RecET from the Rac prophage of E. coli (4), which are particularly efficient in catalyzing homologous recombination between short (Ϸ50 bp) homology segments. The Red recombination functions are encoded by three adjacent genes, gam, bet and exo, in the pL operon (1). RecET functions are encoded by two adjacent genes, recE and recT, present on the cryptic lambdoid Rac prophage found in the genome of many E. coli K-12 strains (5). A variety of studies have concluded that the Exo/Beta and RecE/RecT protein pairs are functionally equivalent although not related at the sequence level (6, 7). Exo and RecE are 5Ј-3Ј exonucleases that degrade the 5Ј ends of linear duplex DNA, creating 3Ј single-strand (ss) DNA overhangs (8, 9). Beta and RecT are single-strand annealing proteins that bind to these ssDNA overhangs and pair them with complementary ssDNA targets (10-12). Gam inhibits two potent host nucleases, RecBCD and SbcCD (13,14), thereby preventing the degradation o...

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“…5). RecE and RecT form part of a minor recombination pathway encoded by a cryptic lambdoid prophage present in some strains of E. coli that is manifest in the absence of a functional RecBCD nuclease/ helicase complex (Clark et al 1993;Kolodner et al 1994). Studies of RecT suggest that despite its independent evolution, it shares with RecA some of the hallmarks of a homologous pairing protein, including the ability to unstack ssDNA bases, which may facilitate homologous contacts (Noirot et al 2003).…”

Section: Homologous Recombination By Exonucleases and Dna-annealing Pmentioning

confidence: 99%

DNA-Pairing and Annealing Processes in Homologous Recombination and Homology-Directed Repair

Morrical

2015

Cold Spring Harb Perspect Biol

116

127

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The formation of heteroduplex DNA is a central step in the exchange of DNA sequences via homologous recombination, and in the accurate repair of broken chromosomes via homology-directed repair pathways. In cells, heteroduplex DNA largely arises through the activities of recombination proteins that promote DNA-pairing and annealing reactions. Classes of proteins involved in pairing and annealing include RecA-family DNA-pairing proteins, single-stranded DNA (ssDNA)-binding proteins, recombination mediator proteins, annealing proteins, and nucleases. This review explores the properties of these pairing and annealing proteins, and highlights their roles in complex recombination processes including the double Holliday junction (DhJ) formation, synthesis-dependent strand annealing, and singlestrand annealing pathways-DNA transactions that are critical both for genome stability in individual organisms and for the evolution of species.

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Genetic and molecular analyses of the C-terminal region of the recE gene from the Rac prophage of Escherichia coli K-12 reveal the recT gene

Cited by 49 publications

References 36 publications

DNA replication triggered by double-stranded breaks in E. coli: Dependence on homologous recombination functions

DNA replication triggered by double-stranded breaks in E. coli: Dependence on homologous recombination functions

Identification and analysis of recombineering functions from Gram-negative and Gram-positive bacteria and their phages

DNA-Pairing and Annealing Processes in Homologous Recombination and Homology-Directed Repair

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