Genetic and molecular regulation of flower pigmentation in soybean

Sundaramoorthy, Jagadeesh; Park, Gyu Tae; Lee, Jeong‐Dong; Kim, Jeong Hoe; Seo, Hak Soo; Song, Jong Tae

doi:10.1007/s13765-015-0077-z

Cited by 16 publications

(19 citation statements)

References 58 publications

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“…Earlier studies on white and white‐hued flowers of G. soja accessions have shown that flower color phenotypes are related to either W1 or W4 (Takahashi et al, 2010, 2012; Yan et al, 2014; Sundaramoorthy et al, 2015). To date, however, there has been no report on the W3 locus in G. soja .…”

Section: Resultsmentioning

confidence: 92%

“…A wide range of flower colors, including dark purple, purple, light purple, pink, magenta, near‐white, and white, have been reported in cultivated soybeans [ Glycine max (L.) Merr.] (Palmer et al, 2004; Sundaramoorthy et al, 2015). The flower color in soybean is controlled primarily by six different genetic loci ( W1 , W2 , W3 , W4 , Wm , and Wp ) (Palmer et al, 2004; Sundaramoorthy et al, 2015).…”

mentioning

confidence: 99%

“…(Palmer et al, 2004; Sundaramoorthy et al, 2015). The flower color in soybean is controlled primarily by six different genetic loci ( W1 , W2 , W3 , W4 , Wm , and Wp ) (Palmer et al, 2004; Sundaramoorthy et al, 2015). W1 , W2 , Wm , and Wp encode flavonoid 3′5′‐hydroxylase (F3′5′H), myeloblastosis (MYB) transcription factor, flavonol synthase, and flavanone 3‐hydroxylase, respectively (Takahashi et al, 2007, 2013; Zabala and Vodkin, 2005, 2007).…”

mentioning

confidence: 99%

“…Dihydroflavonol 4‐reductase catalyzes the reduction of dihydroflavonols to leucoanthocyanins, which are immediate precursors of anthocyanins (Sundaramoorthy et al, 2015). The relationship between flower color and DFR has been studied in many plant species.…”

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confidence: 99%

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Color Variation in a Novel Glycine soja Mutant W4‐S1 with Pinkish‐White Flowers Is Controlled by a Single Recessive Allele at the W4 Locus

et al. 2017

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Dihydroflavonol‐4‐reductase (DFR) plays a crucial role in anthocyanin biosynthesis. In cultivated soybean [Glycine max (L.) Merr.], the W3 and W4 loci encode DFR1 and DFR2, respectively, and are epistatic to each other. In this study, we discovered a new flower color variant of Glycine soja (L.) Merr., CW13133, which has pinkish‐white flowers, and investigated the genetic and molecular basis of the variation in flower color. We found that the W4 locus encoding DFR2 was responsible for the variation in flower color. Sequence analysis of the DFR2 gene of CW13133 revealed three single‐nucleotide polymorphisms (SNPs). Among these, an SNP (T to C) at nucleotide position 2856 was noteworthy, since it led to a change in an amino acid residue (leucine to proline) at position 326 of DFR2. This amino acid substitution was located in a region that is highly conserved among different plant species. The new allele was designated w4‐s1. A cleaved amplified polymorphic sequence analysis showed cosegregation of the w4‐s1 allele with pinkish‐white flowers. Interestingly, DFR1 was not expressed in the w4‐s1 mutant, indicating that the W3 locus has no association with flower color variation. Based on a randomized comparative analysis using 10 different purple‐flowered accessions of G. soja, we provide evidence for the nonassociation of DFR1 expression with total anthocyanin contents and flower color determination in G. soja. In conclusion, we found that the W3 locus in G. soja does not contribute to flower color determination, and consequently there is no epistatic interaction between the W3 and W4 loci, as shown in G. max.

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Section: Resultsmentioning

confidence: 92%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Color Variation in a Novel Glycine soja Mutant W4‐S1 with Pinkish‐White Flowers Is Controlled by a Single Recessive Allele at the W4 Locus

et al. 2017

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“…Plant cultivation, sampling, flavonoid extraction, and HPLC analysis were performed as previously described (Iwashina et al, 2007;Iwashina et al, 2008;Takahashi et al, 2010). To evaluate varietal differences, the peak areas from replicated samples were subjected to a t-test with Statistica software (StatSoft, 2003).…”

Section: Analysis Of Flavonoidsmentioning

confidence: 99%

New Allelic Variant Discovered at Soybean Flower Color Locus W1 Encoding Flavonoid 3′5′‐hydroxylase

Fan

Murai

et al. 2016

Crop Science

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A soybean line producing light purple flowers (E023‐H‐12) was developed from an ethyl methanesulfonate (EMS)‐treated population of cultivar Bay. The objective of this study was to investigate the genetic and molecular basis of flower color variation in E023‐H‐12. Genetic analysis suggested that the W1 gene encoding a flavonoid 3′5′‐hydroxylase (F3′5′H) controls light purple flower color. A single plant with purple flowers was generated in an F2 population derived from a cross between E023‐H‐12 and Clark‐w1 with white flowers, probably because of intragenic recombination of the F3′5′H gene. The allele for light purple flower was designated as w1‐lp2. The dominance relationships of the locus were W1 > w1‐lp2 > w1. Flower petals of E023‐H‐12 had similar expression levels of the F3′5′H gene but they had 42% less anthocyanins compared with Bay. The lower anthocyanin content may account for the light purple color of this mutant line. The nucleotide sequence of the F3′5′H gene of E023‐H‐12 had a single nucleotide polymorphism (SNP) resulting in alteration of an amino acid (H137L). A derived cleaved amplified polymorphic sequence (dCAPS) marker to discriminate the SNP co‐segregated with flower color. Thus, the amino acid substitution may be responsible for the lower anthocyanin content, and, consequently, light purple flower color.

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See the color, see the seed: GmW1 as a visual reporter for transgene and genome editing in soybean

Chen¹,

Yuan²,

Cai³

et al. 2023

The Crop Journal

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Genetic and molecular regulation of flower pigmentation in soybean

Cited by 16 publications

References 58 publications

Color Variation in a Novel Glycine soja Mutant W4‐S1 with Pinkish‐White Flowers Is Controlled by a Single Recessive Allele at the W4 Locus

Color Variation in a Novel Glycine soja Mutant W4‐S1 with Pinkish‐White Flowers Is Controlled by a Single Recessive Allele at the W4 Locus

New Allelic Variant Discovered at Soybean Flower Color Locus W1 Encoding Flavonoid 3′5′‐hydroxylase

See the color, see the seed: GmW1 as a visual reporter for transgene and genome editing in soybean

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