Genetic and morphometric variability in <i>Caiman latirostris</i> (broad‐snouted caiman), Reptilia, Alligatoridae

Amavet, Patricia Susana; Vilardi, Juan C.; Rosso, Esteban; Saidman, Beatriz Ofelia

doi:10.1002/jez.523

Cited by 14 publications

(15 citation statements)

References 39 publications

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“…This structure index is higher than F ST value (0.27) obtained in our previous work (Amavet et al, 2009). The difference in results can be attributed to several causes: (1) in the previous work four populations were taken as units, each represented by 10 non related individuals, whereas in the present study, we used as units groups of related individuals (families represented by the mother and 9-16 hatchlings); (2) the number of individuals analyzed here is much higher than in the previous one; (3) the geographical range sampled here is also wider; (4) the markers differ in their ability to detect variation at different levels as a consequence of dominance, number of alleles, and number of loci analyzed in each case.…”

Section: Discussioncontrasting

confidence: 79%

“…Genetic variability averaged values (Ho = 0.4415 and He = 0.7455) obtained using microsatellite markers are higher than the results of our previous work (He = 0.165) using RAPD markers (Amavet et al, 2009), a result consistent with the usually very high variability associated to microsatellite markers (Queller et al, 1993;Ellegren, 2004).…”

Section: Discussionsupporting

confidence: 78%

“…There are previous works using this method on genus Alligator (Dessauer, unpublished data; Wu et al, 2002), and Crocodylus acutus (Porras Murillo et al, 2008). In Argentina we conducted population genetic studies using RAPD markers and morphometric traits on C. latirostris and we found relatively high estimates of polymorphism and heterozygosity (Amavet et al, 2007(Amavet et al, , 2009.…”

Section: Introductionmentioning

confidence: 99%

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Mating system and population analysis of the broad-snouted caiman (Caiman latirostris) using microsatellite markers

et al. 2012

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The knowledge about reproductive strategies of a species contributes to their conservation. Multiple paternity is a reproductive behavior increasing effective population size, which could increase genetic diversity particularly in populations submitted to bottlenecks events. In Argentina, wild populations of Caiman latirostris are subject of a management plan devoted to their preservation and sustainable utilization based on its commercial interest. This program started in response to the evident numeric reduction of the populations, as a consequence of hunting pressure and habitat modification; it had a remarkable success in population recovery allowing the commercial use of C. latirostris. Data on reproductive behavior of C. latirostris are limited because mating occurs in the water and the information about their genetic diversity is scarce too. Our specific aims were to study the mating system and population genetic structure applying microsatellite markers in twelve C. latirostris families. The obtained results showed highly significant difference among populations and a lack of correspondence between geographical distance and genetic differentiation suggesting that populations of C. latirostris represent unstable metapopulations. In the paternity analysis was detected more than one father in two nests, which could be explained by capacity of storage sperm, proposed in females of a related species. The behavior of multipaternity could contribute to maintain viable populations of C. latirostris, since the maintenance of genetic variability within populations could help increase their capacity to respond to selective pressure. Further studies employing genetic and behavioral framework are needed to better understand the reproductive biology of C. latirostris.

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Section: Discussioncontrasting

confidence: 79%

Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

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Mating system and population analysis of the broad-snouted caiman (Caiman latirostris) using microsatellite markers

et al. 2012

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“…RAPD-PCR amplifies DNA segments of variable lengths and such length polymorphisms are inherited in a Mendelian fashion and thus can be used as genetic markers [10]. RAPD analysis has been extensively used to evaluate genetic diversity in Brycon lundii, Astyanax altiparanae and Prochilodus marggravii [11][12][13], flounder [14], Labeo rohita [15], catfish [16,17], mud eel [18], ornamental reef fishes [19], crocodile [20], black rat snake [21], and hilsa [22]; and in subspecies identification of tilapia [23], Badis badis, Dario dario [24], and lamprey [25]. A very limited genomic research has been carried out in Badis badis species till date and insufficient genomic information is available to perform other sophisticated fingerprinting techniques where whole genomic sequence is necessary; therefore, RAPD fingerprinting is the best choice for genetic diversity analysis in this species.…”

Section: Introductionmentioning

confidence: 99%

Study of the Genetic Diversity of the Ornamental FishBadis badis(Hamilton-Buchanan, 1822) in the Terai Region of Sub-Himalayan West Bengal, India

Mukhopadhyay

Bhattacharjee

2014

International Journal of Biodiversity

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Dwarf chameleon fish or Badis badis, a lesser known ornamental freshwater fish, has recently been included in the Indian threatened category of fish list. There are insufficient studies with regard to the assessment of genetic background of this ichthyofauna, especially in the western sub-Himalayan region of West Bengal, India, popularly known as the Terai. The present study is the first attempt to investigate the present status of the genetic background of this species in the Mahananda and Balason rivers, major streams of this region. Twenty-one selective RAPD primers generated 53 and 60 polymorphic fragments in the Mahananda and Balason populations, respectively. The proportion of polymorphic loci, Nei's genetic diversity (H), and Shannon's index ( ) were 0.4416, 0.1654 ± 0.2023, and 0.2450 ± 0.2907, respectively, in Mahananda river population and were 0.5041, 0.1983 ± 0.2126, and 0.2901 ± 0.3037, respectively, in Balason river population. Inbreeding coefficient and degree of gene differentiation were also calculated. The H and were found to be 0.1601±0.1944 and 0.2363±0.2782, respectively, in overall Mahananda-Balason river system. Our study revealed considerable lack of genetic variation among the individuals of Badis badis. The genetic data obtained from the present study lend support to the view that there is a scope of stock improvement for this ichthyofauna.

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“…Molecular biology techniques provide useful tools for rapid analysis and specific identification. Molecular markers based on DNA sequence (such us random amplified polymorphic DNA or RAPD) have been widely used to study genetic variation and population structure in these species, whose genomes have not been well characterized (Welsh and McClelland, 1990;Amavet et al, 2007Amavet et al, , 2009. The application of these methods would help to discriminate between different genomes, taking into account that their skins are frequently indistinguishable along the marketing chain.…”

Section: Introductionmentioning

confidence: 99%

DNA extraction from skins of wild (Hydrochoerus hydrochaeris and Pecari tajacu) and domestic (Sus scrofa domestica) species using a novel protocol

Ojeda

Amavet²,

Rueda³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. Sometimes, commercial products obtained from wild animals are sold as if they were from domestic animals and vice versa. At this point of the productive chain, legal control of possible wildlife products is difficult. Common in the commerce of northern Argentina, skins of two wild species, the carpincho and the collared peccary, look very similar to each other and to those of the domestic pig; it is extremely difficult to differentiate them after they have been tanned. Because there was no an adequate methodology to discriminate between leather of these three species, we developed a new methodology of DNA extraction from skin and leather. This new method involves digesting a leather sample using proteinase K, followed by precipitation of proteins with 5 M NaCl, cleaning with absolute isopropanol and DNA precipitation with 70% ethanol. DNA is hydrated in Tris-EDTA buffer. This protocol provided good-quality DNA suitable for analysis with molecular markers. This new protocol has potential for use in DNA extraction from animal skins using a novel protocol identifying leather products of these species using molecular markers based on RAPDs.

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Genetic and morphometric variability in Caiman latirostris (broad‐snouted caiman), Reptilia, Alligatoridae

Cited by 14 publications

References 39 publications

Mating system and population analysis of the broad-snouted caiman (Caiman latirostris) using microsatellite markers

Mating system and population analysis of the broad-snouted caiman (Caiman latirostris) using microsatellite markers

Study of the Genetic Diversity of the Ornamental FishBadis badis(Hamilton-Buchanan, 1822) in the Terai Region of Sub-Himalayan West Bengal, India

DNA extraction from skins of wild (Hydrochoerus hydrochaeris and Pecari tajacu) and domestic (Sus scrofa domestica) species using a novel protocol

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