Genetic and pathogenic diversity of severe fever with thrombocytopenia syndrome virus (SFTSV) in South Korea

Yun, Seok‐Min; Park, Su Jin; Kim, Young Il; Park, Sun Whan; Yu, Min; Kwon, Hyeok Il; Kim, Eun Ha; Yu, Kwang Min; Jeong, Hye Won; Ryou, Jungsang; Lee, Won Ja; Jee, Youngmee; Lee, Joo Yeon; Choi, Young Ki

doi:10.1172/jci.insight.129531

Cited by 79 publications

(90 citation statements)

References 36 publications

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“…Four of 5 SFTSV-positive patients were <15 years of age, and all 5 recovered within a week. Given that the disease severity of SFTS is associated with host age and the viral genotype (15), milder clinical symptoms observed in these patients might have been because of exposure at a younger age or prevalence of less virulent genotypes of SFTSV in Myanmar. Therefore, continuous surveillance of SFTS patients needs to be conducted, reporting detailed clinical manifestations and associated viral genotypes prevalent in the local area.…”

Section: Discussionmentioning

confidence: 99%

Genotypic Heterogeneity of Orientia tsutsugamushi in Scrub Typhus Patients and Thrombocytopenia Syndrome Co-infection, Myanmar

Win¹,

Nguyen

Kim

et al. 2020

Emerg. Infect. Dis.

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Section: Discussionmentioning

confidence: 99%

Genotypic Heterogeneity of Orientia tsutsugamushi in Scrub Typhus Patients and Thrombocytopenia Syndrome Co-infection, Myanmar

Win¹,

Nguyen

Kim

et al. 2020

Emerg. Infect. Dis.

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“…The overlapping time and geographical location of sample isolations suggest that the soldier may have been infected by a strain closely related to USAMRIID-HLP23. However, additional geographical information of closely related strains is needed to support this claim [38].…”

Section: Discussionmentioning

confidence: 99%

“…Individual coding sequences were concatenated in Geneious version R9 ( ) to form coding-complete genomes. Strains with confirmed reassortments were removed from downstream Bayesian analyses [ 38 ]. GenBank accession numbers and metadata can be found in Table S1 .…”

Section: Methodsmentioning

confidence: 99%

“…AFMC-17 is most closely related to KOR strain 16MS344, whereas the tick-related strains were closely related to KOR strains 16KS100 and 16KS104. No geographical sampling data are publicly available of closely related strains [38]. Time to the most recent common ancestor (tMRCA) and the 95% highest posterior densities (HPD) are shown.…”

Section: Phylogenetic Analysismentioning

confidence: 99%

“…Tree branches are scaled by substitutions per site per year. The inset tree (B) contains recently published sequences from KOR that are not included in (A) [38].…”

Section: Phylogenetic Analysismentioning

confidence: 99%

See 2 more Smart Citations

A Model for the Production of Regulatory Grade Viral Hemorrhagic Fever Exposure Stocks: From Field Surveillance to Advanced Characterization of SFTSV

Pérez-Sautu

Caviness

et al. 2020

Viruses

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Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging human pathogen, endemic in areas of China, Japan, and the Korea (KOR). It is primarily transmitted through infected ticks and can cause a severe hemorrhagic fever disease with case fatality rates as high as 30%. Despite its high virulence and increasing prevalence, molecular and functional studies in situ are scarce due to the limited availability of high-titer SFTSV exposure stocks. During the course of field virologic surveillance in 2017, we detected SFTSV in ticks and in a symptomatic soldier in a KOR Army training area. SFTSV was isolated from the ticks producing a high-titer viral exposure stock. Through the use of advanced genomic tools, we present here a complete, in-depth characterization of this viral stock, including a comparison with both the virus in its arthropod source and in the human case, and an in vivo study of its pathogenicity. Thanks to this detailed characterization, this SFTSV viral exposure stock constitutes a quality biological tool for the study of this viral agent and for the development of medical countermeasures, fulfilling the requirements of the main regulatory agencies.

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A natural variation in the RNA polymerase of severe fever with thrombocytopenia syndrome virus enhances viral replication and in vivo virulence

Jeon,

Ro,

Kang

et al. 2023

Journal of Medical Virology

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Severe fever with thrombocytopenia syndrome (SFTS) is an emerging tick‐borne disease with high mortality in Eastern Asia. The disease is caused by the SFTS virus (SFTSV), also known as Dabie bandavirus, which has a segmented RNA genome consisting of L, M, and S segments. Previous studies have suggested differential viral virulence depending on the genotypes of SFTSV; however, the critical viral factor involved in the differential viral virulence is unknown. Here, we found a significant difference in viral replication in vitro and virulence in vivo between two Korean isolates belonging to the F and B genotypes, respectively. By generating viral reassortants using the two viral strains, we demonstrated that the L segment, which encodes viral RNA‐dependent RNA polymerase (RdRp), is responsible for the enhanced viral replication and virulence. Comparison of amino acid sequences and viral replication rates revealed a point variation, E251K, on the surface of RdRp to be the most significant determinant for the enhanced viral replication rate and in vivo virulence. The effect of the variation was further confirmed using recombinant SFTSV generated by reverse genetic engineering. Therefore, our results indicate that natural variations affecting the viral replicase activity could significantly contribute to the viral virulence of SFTSV.

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Genetic and pathogenic diversity of severe fever with thrombocytopenia syndrome virus (SFTSV) in South Korea

Cited by 79 publications

References 36 publications

Genotypic Heterogeneity of Orientia tsutsugamushi in Scrub Typhus Patients and Thrombocytopenia Syndrome Co-infection, Myanmar

Genotypic Heterogeneity of Orientia tsutsugamushi in Scrub Typhus Patients and Thrombocytopenia Syndrome Co-infection, Myanmar

A Model for the Production of Regulatory Grade Viral Hemorrhagic Fever Exposure Stocks: From Field Surveillance to Advanced Characterization of SFTSV

A natural variation in the RNA polymerase of severe fever with thrombocytopenia syndrome virus enhances viral replication and in vivo virulence

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