Genetic and physical characterization of P1dlw prophage and its derivatives

Structures of oversized genomes of phage P1 derivatives carrying lac genes of Escherichia coli K12

“…3B. It is worthy to note that the chromosomal markers in the majority of the specialized transducing P1 phages isolated reside in the proAb-argF-lac region [2][3][4][5][6]. It is likely that the IS/ sequence present in this region [20] provide sites for integration of the P1 genome as the first step for DNA rearrangements leading to the production of specialized transducing P1 phage derivatives.…”

“…The Pllac derivatives isolated so far are oversized, i.e. their genomes are larger than the size of DNA molecules packageable into the P1 virion, but their genome structures have not been well characterized [2,3,6]. We analyse here the structures of two oversized Plac genomes isolated in our laboratory and that of a third Pllac which has been isolated elsewhere [3].…”

Regulation of plasmid replication.

“…Before in vitro recombinant technology was available, the first mini-Pl replicons were isolated as specialized transducing particles produced spontaneously. One, called pIH1972, contaihs the P1 fragment called EcoRI-5 and P1 DNA to the left of this fragment (161,162). In addition, two different classes of deletion plasmids, obtained in vivo in a X vector, contain the EeoRI-5 fragment and P1 DNA either to its right (5R) or to its left (5L) (182).…”