Genetic and physical map of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pJP4

Don, R. H.; Pemberton, John

doi:10.1128/jb.161.1.466-468.1985

Cited by 161 publications

(43 citation statements)

References 9 publications

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“…Recently, cross-binding has been reported between ClcR, TcbR, which is the regulator of the tcbCDEF operon that specifies chlorocatechol degradation in Pseudomonas sp. strain P51(pP51) (van der Meer et al, 1991), and TfdR, which is the regulator of the tfdCDEF operon that encodes chlorocatechol degradation in Ralstonia eutropha JMP134(pJP4) (Don and Pemberton, 1985;Perkins et al, 1990;Leveau and van der Meer, 1996). These three proteins are highly homologous and regulate very similar systems.…”

Section: Discussionmentioning

confidence: 99%

DNase I footprinting, DNA bending and in vitro transcription analyses of ClcR and CatR interactions with the clcABD promoter: evidence of a conserved transcriptional activation mechanism

McFall

Klem

Fujita

et al. 1997

Molecular Microbiology

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SummaryIn Pseudomonas putida, benzoate and 3-chlorobenzoate are converted to catechol and 3-chlorocatechol, respectively, which are then catabolized to tricarboxylic acid cycle intermediates via the catBCA and clcABD pathways. The catBCA and clcABD operons are regulated by homologous transcriptional activators CatR and ClcR. Previous studies have demonstrated that in addition to sequence similarities, CatR and ClcR share functional similarities which allow catR to complement clcR. In this study, we demonstrate that CatR activates the clcABD promoter in vitro without inducer, but more transcript is produced when inducer is added. DNase I footprinting and DNAbending analyses demonstrate that CatR binds to and bends the clcABD promoter to the same angle as does ClcR plus its inducer, 2-chloromuconate. This implies that CatR binds to the clc promoter in its active conformation. Transcription of the clcABD promoter by the ␣-subunit truncation mutant (␣-235) of RNA polymerase was sharply reduced, indicating that the ␣-subunit C-terminal domain is important. However, a small amount of transcript was produced under these conditions, indicating that other contact sites on the RNA polymerase may play a role in activation.

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Section: Discussionmentioning

confidence: 99%

DNase I footprinting, DNA bending and in vitro transcription analyses of ClcR and CatR interactions with the clcABD promoter: evidence of a conserved transcriptional activation mechanism

McFall

Klem

Fujita

et al. 1997

Molecular Microbiology

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“…The best-described pathway for 2,4-D degradation, both genetically and biochemically, is the plasmid-encoded pathway of the saprophytic soil bacterium Alcaligenes eutrophus JMP134 that was isolated in Australia (39). This organism harbors an 88-kb self-conjugable plasmid (pJP4), which encodes all of the structural and regulatory genes needed to convert 2,4-D to 2-chloromaleylacetic acid, namely, tfdA through tfdF, tfdR, and tfdS (11,12,20,(28)(29)(30)37). This intermediate is subsequently reduced by the chromosomal mar gene product to ␤-ketoadipate, which is further metabolized by chromosomally encoded gene products to ultimately yield CO 2 (32).…”

mentioning

confidence: 99%

Characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC

Suwa

Wright

Fukimori

et al. 1996

Appl Environ Microbiol

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The findings of previous studies indicate that the genes required for metabolism of the pesticide 2,4dichlorophenoxyacetic acid (2,4-D) are typically encoded on broad-host-range plasmids. However, characterization of plasmid-cured strains of Burkholderia sp. strain RASC, as well as mutants obtained by transposon mutagenesis, suggested that the 2,4-D catabolic genes were located on the chromosome of this strain. Mutants of Burkholderia strain RASC unable to degrade 2,4-D (2,4-D ؊ strains) were obtained by insertional inactivation with Tn5. One such mutant (d1) was shown to have Tn5 inserted in tfdA RASC , which encodes 2,4-D/␣ketoglutarate dioxygenase. This is the first reported example of a chromosomally encoded tfdA. The tfdA RASC gene was cloned from a library of wild-type Burkholderia strain RASC DNA and shown to express 2,4-D/␣ketoglutarate dioxygenase activity in Escherichia coli. The DNA sequence of the gene was determined and shown to be similar, although not identical, to those of isofunctional genes from other bacteria. Moreover, the gene product (TfdA RASC ) was purified and shown to be similar in molecular weight, amino-terminal sequence, and reaction mechanism to the canonical TfdA of Alcaligenes eutrophus JMP134. The data presented here indicate that tfdA genes can be found on the chromosome of some bacterial species and suggest that these catabolic genes are rather mobile and may be transferred by means other than conjugation.

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“…Typical naturally occurring Inc-P1 plasmids confer resistance to more than one antimicrobial agent, with the exception of plasmid pJP4, which was isolated because it confers Alcaligenes the ability to catabolise the pesticide 2,4-dichlorophenoxyacetic acid and also to code for the resistance to mercury salts [18]. We have been able to show that plasmid pAMJ6 codes for resistance to mercury salts; nevertheless, plasmid pAMJ6 does not appear to code for any other resistance properties from strain R6.…”

Section: Lncompatibifity Groupingmentioning

confidence: 81%

Isolation and characterization of a mercury-resistant broad-host-range plasmid from Pseudomonas cepacia

Sabaté

1994

FEMS Microbiology Letters

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A plasmid which codifies a mercury resistant phenotype was isolated from Pseudomonas cepacia. Evidence was obtained for conjugative transfer of the mercury resistant phenotype to a P. putida strain. The plasmid (termed pAMJ6) was transferred by conjugation and transformation to various enterobacterial strains and also mobilized from them. In all conjugant and transformant strains it was possible to recover plasmid pAMJ6. This plasmid seems to belong to the Inc-P1 group.

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Genetic and physical map of the 2,4-dichlorophenoxyacetic acid-degradative plasmid pJP4

Cited by 161 publications

References 9 publications

DNase I footprinting, DNA bending and in vitro transcription analyses of ClcR and CatR interactions with the clcABD promoter: evidence of a conserved transcriptional activation mechanism

DNase I footprinting, DNA bending and in vitro transcription analyses of ClcR and CatR interactions with the clcABD promoter: evidence of a conserved transcriptional activation mechanism

Characterization of a chromosomally encoded 2,4-dichlorophenoxyacetic acid/alpha-ketoglutarate dioxygenase from Burkholderia sp. strain RASC

Isolation and characterization of a mercury-resistant broad-host-range plasmid from Pseudomonas cepacia

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