Genetic and Population Analyses of
            <i>Vibrio Parahaemolyticus</i>
            Isolates from Three Major Coastal Regions in China

Li, Ping; Xin, Wenwen; Kang, Lin; Chen, Zhongwen; Guo, Changhong; Gao, Shan; Yang, Hao; Wang, Henghui; Zhou, Dongsheng; Yang, Wenhui; Wang, Jinglin

doi:10.2217/fmb-2018-0060

Cited by 6 publications

(2 citation statements)

References 27 publications

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“…21 V parahaemolyticus isolated from 3 main water areas in China has different genetic diversity and population structures. 20,22 Based on the analysis using WGS, there were differences in the pathogenicity of V parahaemolitycus in various aquatic environments resulting from the combination of horizontal genome transfer, pathological distribution elements. 11,13,18 V. parahaemolyticus was also found to cause death in shrimp due to acute hepatopancreatic necrosis disease.…”

Section: Resultsmentioning

confidence: 99%

Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa

Kartika

Fitria

Oktaviola

2021

J. Teknol. Laboratorium

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Caulerpa racemosa is a green algae consumed by people in northern coastal areas. C. racemosa has a habitat attached to the shallow seabed. C. racemosa usualy consumed fresh without any cooking process so that the contamination of microorganisms can be eaten. Molecular identification using 16S rRNA is needed to determine the type of bacterial contaminants in C. racemosa. The isolates of C. racemosa were cultured in HIA, BAP, and BHI media. Bacteria from BHI media were isolated by DNA, PCR for 16S rRNA gene, and sequencing. Bacteria isolate C. racemosa was found to have the α-hemolytic ability in BAP media. The sequencing analysis showed that the three bacterial colonies of C. racemosa isolate had high similarity with V. parahemolyticus, Caldalkalibacillus mannanilyticus, and Exiguobacterium profundum.

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Section: Resultsmentioning

confidence: 99%

Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa

Kartika

Fitria

Oktaviola

2021

J. Teknol. Laboratorium

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“…The recA gene of two strains (19-VB00022 and 19-VB00023) contained insertions within the 3' coding region of the recA gene, so only 404 bp instead of 462 bp of the recA gene were used for the construction of all concatemers (truncation of 3' sequences of recA). Insertions into coding regions of recA genes were also observed in a number of V. parahaemolyticus strains [29] and make it impossible to assign an allele number and sequence type for those strains.…”

Section: Multilocus Sequence Analysis (Mlsa) Of Housekeeping Genesmentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Veterinary Vibrio cincinnatiensis Isolates

et al. 2020

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Vibrio cincinnatiensis is a halophilic species which has been found in marine and estuarine environments worldwide. The species is considered a rare pathogen for which the significance for humans is unclear. In this study, nine veterinary isolates were investigated that were obtained from domestic animals in Germany. The isolates were mostly recovered from abortion material of pigs, cattle, and horse (amnion or fetuses). One isolate was from a goose. A human clinical strain from a case of enteritis in Germany described in the literature was also included in the study. Whole-genome sequencing (WGS) of all isolates and MALDI-TOF MS (matrix-assisted-laser-desorption/ionization time-of-flight mass spectrometry) were performed to verify the species assignment. All strains were investigated for phenotypic traits including antimicrobial resistance (AMR), biochemical properties, and two virulence-associated phenotypes (hemolytic activity and resistance to human serum). WGS data and MS spectra confirmed that all veterinary isolates are closely related to the type strain V. cincinnatiensis NCTC12012. An exception was the human isolate from Germany which is related to the other isolates but could belong to another species. The isolates were similar in most biochemical phenotypes. Only one strain showed a very weak hemolytic activity against sheep erythrocytes, and serum resistance was intermediate in two strains. AMR phenotypes were more variable between the isolates. Resistances were observed against ß-lactams ampicillin and cefoxitin and against tetracycline and the sulfonamide antibiotics trimethoprim and sulfamethoxazole. Some acquired AMR genes were identified by bioinformatics analyses. WGS and MALDI-TOF MS data reveal a close relationship of the veterinary isolates and the type strain V. cincinnatiensis NCTC12012, which is a clinical human isolate. As the veterinary isolates of this study were mostly recovered from abortion material (amnions and fetuses), a zoonotic potential of the veterinary isolates seems possible.

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Virulence Genes Analysis of Vibrio parahaemolyticus and Anti-vibrio Activity of the Citrus Extracts

Singhapol

Tinrat

2020

Curr Microbiol

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Genetic and Population Analyses of Vibrio Parahaemolyticus Isolates from Three Major Coastal Regions in China

Cited by 6 publications

References 27 publications

Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa

Molecular identification of pathogenic bacteria causing foodborne disease in Caulerpa racemosa

Phenotypic and Genotypic Characterization of Veterinary Vibrio cincinnatiensis Isolates

Virulence Genes Analysis of Vibrio parahaemolyticus and Anti-vibrio Activity of the Citrus Extracts

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