Genetic and population diversity of Toxocara cati (Schrank, 1788) Brumpt, 1927, on the basis of the internal transcribed spacer (ITS) region

Venkatesan, Thangam; Panda, Rasmita; Kumari, Ansu; Nehra, Anil Kumar; Ram, Hira; Pateer, Devendra Prasad; Karikalan, M.; Garg, Rajat; Singh, Mamta; Shukla, Utkarsh; Pawde, A. M.

doi:10.1007/s00436-022-07671-9

Cited by 6 publications

(1 citation statement)

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“…The effectiveness of PCR in identifying eggs in feces is directly related to the number of eggs available, making it difficult to use as a routine diagnostic tool for canine ascarid infections, given the low egg concentration (Öge et al, 2019). Another possible factor is the resistance of ascarid eggshells due to their lipid composition (ascaroside), which covers the inner surface of the chitinous layer (Venkatesan et al, 2022).…”

Section: Coproparasitological Examination Of Domestic Dogsmentioning

confidence: 99%

Diversity of helminths with zoonotic potential and molecular characterization of Toxocara canis infecting domestic dogs from locations of Amazon and Atlantic Forest Brazilian biomes

Dias-Correia,

Neves,

Bittencourt-Oliveira

et al. 2023

Rev. Bras. Parasitol. Vet.

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The coproparasitological examination of dogs (n=278) from two Brazilian biomes (Amazon [AZ] and Atlantic Forest [AF]) by centrifugal flotation demonstrated positivity values of 54.2% (AF) and 48.5% (AZ). The most prevalent parasites in AF were hookworms (81.0% - 47/58), Toxocara sp. (17.3% - 10/58) and Trichuris vulpis (12.1% - 7/58); while in AZ they were hookworms (86.7% - 72/83), Toxocara sp. (18.1% - 15/83), Dipylidium caninum (13.3% - 11/83) and T. vulpis (10.8% - 9/83). PCR was performed using the partial mitochondrial genes cytochrome c oxidase subunit 1 (pcox1) and NADH dehydrogenase 1 (pnad1) in 25 fecal samples positive for Toxocara sp. eggs and found one sample positive for pcox1 and six positives for pnad1. The sequencing of these samples was unsuccessful due to the difficulties inherent in copro-PCR+sequencing. The sequencing of 14 samples of T. canis adult helminths retrieved 11 sequences of 414 bp for pcox1 and nine sequences of 358 bp for pnad1. The phylogenetic trees of these sequences confirmed the species T. canis. Intraspecific genetic variation was only observed for pnad1. This is the second study involving molecular analysis of T. canis in dogs from Brazil and adds new information through the use of pnad1.

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Section: Coproparasitological Examination Of Domestic Dogsmentioning

confidence: 99%