Genetic and reproductive toxicity of aqueous extracts of Telfairia occidentalis (Hook F.), Vernonia amygdalina and their combination on the testicular cells of male mice

Oyinleye, O. E.; Adeniran, S. A.; Ogunsuyi, Opeoluwa M.; Oyeyemi, Ifeoluwa T.; Bakare, Adekunle A.

doi:10.1007/s13596-020-00507-w

Cited by 5 publications

(4 citation statements)

References 34 publications

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“…Four doses: ×0.25, ×0.5, ×1 and ×2.0 of the standard daily dose corresponding respectively to 6.25, 12.5, 25 and 50 mg/kg bw of PZA; 2.5, 5.0, 10, and 20 mg/kg bw of RIF; 1.25, 2.5, 5.0 and 10 mg/kg bw of INH; 3.75, 7.5, 15 and 30 mg/kg bw of EMB; and 7, 14, 28 and 56 mg/kg bw of FDC ( Table 1 ) were considered. The administration and duration of animal exposure were carried out in accordance with standard protocols ( Wyrobek et al, 1983 ; Ogunsuyi et al, 2020 ; Oyinleye et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…The design of Wyrobek et al (1983) , with minor adjustment ( Ogunsuyi et al, 2020 ; Oyinleye et al, 2021 ) was utilized. There were 22 groups of mice [ n = 8 (5 for the sperm morphology assay and 3 for the testes weight and histopathology) ( Table 1 )].…”

Section: Methodsmentioning

confidence: 99%

“…The slides were thereafter air-dried and arranged in groups for the subsequent microscopic examination at ×1000. One thousand spermatozoa from each mouse were examined for morphological abnormalities ( Ogunsuyi et al, 2020 ; Oyinleye et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

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The first-line antituberculosis drugs, and their fixed-dose combination induced abnormal sperm morphology and histological lesions in the testicular cells of male mice

Bakare

Moses

Beckely

et al. 2022

Front. Cell Dev. Biol.

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Rifampicin (RIF), Isoniazid (INH), Ethambutol (EMB), Pyrazinamide (PZA), and/or their fixed-dose combination (FDC) are extensively prescribed in the cure of Tuberculosis (TB) globally. In spite of the beneficial effect, these drugs are capable of inducing cellular toxicity. Existing information on the genotoxic effects of the first-line anti-TB drugs is limited and contentious. Herein, we evaluated the reproductive genotoxicity of RIF, INH, EMB, PZA, and their FDC utilizing the mouse sperm morphology assay. Histological examination of the testes of exposed mice was also performed. Male Swiss albino mice (11–13 weeks old) were intraperitoneally exposed for 5 consecutive days to each of the anti-TB drugs at four different doses of 6.25, 12.5, 25, and 50 mg/kg bw of PZA; 2.5, 5.0, 10, and 20 mg/kg bw of RIF; 1.25, 2.5, 5.0 and 10 mg/kg bw of INH; 3.75, 7.5, 15 and 30 mg/kg bw of EMB; and 7, 14, 28 and 56 mg/kg bw of FDC corresponding respectively to ×0.25, ×0.5, ×1 and ×2.0 of the standard daily dose. In comparison with the negative control (normal saline), there was no significant difference in the testicular weight and organo-somatic index of exposed mice. There was an increase (p > 0.05) in the frequency of abnormal spermatozoa at most of the tested doses of each drug and a dose-dependent decrease with the FDC. Each of the anti-TB drugs except the FDC induced pathological lesions in the testes. These findings suggest that the individual first-line anti-TB drug unlike the FDC has the potential to provoke testicular anomalies in male mice.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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The first-line antituberculosis drugs, and their fixed-dose combination induced abnormal sperm morphology and histological lesions in the testicular cells of male mice

Bakare

Moses

Beckely

et al. 2022

Front. Cell Dev. Biol.

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“…A small section of each liver was cut and preserved in 10% formalin for histopathological analysis as earlier reported (Oyinleye et al 2021).…”

Section: Histopathological Analysismentioning

confidence: 99%

Hepatoprotective effect of the methanol extract of Luffa cylindrica fruit on carbon-tetrachloride induced chronic liver injury

et al. 2023

Self Cite

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Luffa cylindrica (Linn) commonly called sponge gourds has both medicinal and nutritional properties. It is used traditionally for the management of liver diseases. Herein, we investigated the hepatoprotective effect of the crude methanol extract of L. cylindrica fruit in rats chronically exposed to carbon tetrachloride (CCl4). Male rats were exposed to CCl4 twice a week for six weeks and the extract was administered five times a week for six weeks. Markers of liver toxicity, antioxidant enzymes and liver peroxidation were evaluated and histological analysis of the liver was carried out. Significant reduction in serum markers (ALT, AST and ALP), increase in antioxidant enzyme and reduction in lipid peroxidation compared to CCl4 were observed in rats exposed to both CCl4 and the extract. CCl4-induced liver lesions were ameliorated by the extract. These show the protective effect of the methanol extract of L. cylindrica on CCl4-induced chronic liver injury in rats.

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Aqueous extract of Moringa oleifera Lam. induced mitodepression and chromosomal aberration in Allium cepa, and reproductive genotoxicity in male mice

Bakare

Akpofure

Gbadebo

et al. 2021

ADV TRADIT MED (ADTM)

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Genetic and reproductive toxicity of aqueous extracts of Telfairia occidentalis (Hook F.), Vernonia amygdalina and their combination on the testicular cells of male mice

Cited by 5 publications

References 34 publications

The first-line antituberculosis drugs, and their fixed-dose combination induced abnormal sperm morphology and histological lesions in the testicular cells of male mice

The first-line antituberculosis drugs, and their fixed-dose combination induced abnormal sperm morphology and histological lesions in the testicular cells of male mice

Hepatoprotective effect of the methanol extract of Luffa cylindrica fruit on carbon-tetrachloride induced chronic liver injury

Aqueous extract of Moringa oleifera Lam. induced mitodepression and chromosomal aberration in Allium cepa, and reproductive genotoxicity in male mice

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