Genetic and Serologic Properties of Zika Virus Associated with an Epidemic, Yap State, Micronesia, 2007

Lanciotti, Robert S.; Kosoy, Olga; Laven, Janeen; Velez, Jason O.; Lambert, Amy J.; Johnson, Alison J.; Stanfield, Stephanie M.; Duffy, Mark R.

doi:10.3201/eid1408.080287

Cited by 2,001 publications

(2,306 citation statements)

References 19 publications

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“…Viral loads of all patient samples, and all HC sera with equivocal or positive ZIKV IgM, were tested by quantitative real‐time polymerase chain reaction (qRT‐PCR) targeting both the Asian and African ZIKV lineage (ZIKV_1086_fwd, ZIKV_1107_probe and ZIKV_1162c) 23. The MagnaPureLC system (Roche Diagnostics, Almere, The Netherlands) was used to extract total nucleic acid from 50 μ L serum.…”

Section: Methodsmentioning

confidence: 99%

Zika virus and Guillain–Barré syndrome in Bangladesh

GeurtsvanKessel

Islam

et al. 2018

Ann Clin Transl Neurol

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ObjectivePrevious studies have associated Guillain–Barré syndrome (GBS) with Zika virus (ZIKV) outbreaks in South America and Oceania. In Asia, ZIKV is known to circulate widely, but the association with Guillain–Barré syndrome is unclear. We investigated whether endemic ZIKV infection is associated with the development of GBS.MethodsA prospective study was conducted from 2011 to 2015 in Bangladesh. A total of 418 patients and 418 healthy family controls were included in the study. Patients were diagnosed with GBS prior to inclusion according to established criteria. Detailed information on the epidemiology, clinical presentation, electrophysiology, diagnosis, disease severity, and clinical course were obtained during a follow‐up of 1 year using a predefined protocol.Results ZIKV‐neutralizing antibodies were detected in our study from 2013 onwards. The prevalence of ZIKV‐neutralizing antibodies was not significantly higher in patients with GBS compared to healthy controls (OR 2.23, P = 0.14, 95% CI 0.77–6.53). Serological evidence for prior ZIKV infection in patients with GBS was associated with more frequent cranial, sensory, and autonomic nerve involvement compared to GBS patients with Campylobacter jejuni, the predominant preceding infection in GBS worldwide. Nerve‐conduction studies revealed that ZIKV antibodies were associated with a demyelinating subtype of GBS, while C. jejuni infections were related to an axonal subtype.InterpretationNo significant association was found between ZIKV infection and GBS in Bangladesh, but GBS following ZIKV infection was characterized by a distinct clinical and electrophysiological subtype compared to C. jejuni infection. These findings indicate that ZIKV may precede a specific GBS subtype but the risk is low.

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Section: Methodsmentioning

confidence: 99%

Zika virus and Guillain–Barré syndrome in Bangladesh

GeurtsvanKessel

Islam

et al. 2018

Ann Clin Transl Neurol

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“…In total, 131 samples (68 from saliva and 63 from urine) were collected and analyzed from 69 Brazilian subjects of all ages that met the inclusion criteria of presenting specific symptoms and signs for ZIKV (acute phase), such as fever, cutaneous rash, headache, conjunctivitis, myalgia and arthralgia; and had confirmed positive for ZIKV and negative for Dengue and Chikungunya virus infection by blood analysis utilizing qPCR, following the criteria described by Lanciotti et al 2008 [16]. The samples were collected during the acute phase of the disease and the average days between the onset of symptoms and sample collection were 3.2 ± 2.9 days.…”

Section: Methodsmentioning

confidence: 99%

Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Castro

Sabalza

Barber

et al. 2018

Journal of Oral Microbiology

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Background: Zika virus (ZIKV) is a single-stranded RNA virus and member of the Flaviviridae family. Recent studies have reported that saliva can be an important alternative to detect ZIKV. Saliva requires less processing than blood greatly simplifying the assay. Loop-mediated Isothermal Amplification (LAMP) is a rapid assay that detects nucleic acids, including ZIKV RNA. Aim: The aim of this study was to evaluate the efficacy of saliva and urine to diagnose ZIKV infection in subjects during the acute phase, through ZIKV RNA detection by LAMP. Method: A total of 131 samples (68 saliva and 63 urine samples) from 69 subjects in the acute phase of ZIKV infection, and confirmed positive for ZIKV by blood analysis through real time-PCR, were collected and analyzed by Reverse Transcriptase Loop-mediated Isothermal Amplification (RT-LAMP). Results: From the 68 saliva samples, 45 (66.2%) were positive for ZIKV with an average time to positivity (Tp) of 13.5 min, and from the 63 urine samples, 25 (39.7%) were positive with the average Tp of 15.8 min. Saliva detected more samples (p = 0.0042) and had faster Tp (p = 0.0176) as compared with urine. Conclusion: Saliva proved to be a feasible alternative to diagnose ZIKV infection during the acute phase by LAMP.

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“…Subsequently, ZIKV spread to countries in Oceania and the Americas. The first large outbreak of Zika disease in humans occurred on the Pacific island of Yap in the Federated States of Micronesia in 2007 (Lanciotti et al, 2008;Duffy et al, 2009). ZIKV was then identified from Suriname (Enfissi et al, 2016b) in South America, and on other Pacific Islands including French Polynesia (Berthet et al, 2014;Cao-Lormeau et al, 2014;Hancock et al, 2014), Easter Island of Chile (Tognarelli et al, 2015), the Cook Islands (Roth et al, 2014), and New Caledonia (Roth et al, 2014;Dupont-Rouzeyrol et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic analysis revealed the central roles of two African countries in the evolution and worldwide spread of Zika virus

et al. 2016

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Recent outbreaks of Zika virus (ZIKV) infections inOceania's islands and the Americas were characterized by high numbers of cases and the spread of the virus to new areas. To better understand the origin of ZIKV, its epidemic history was reviewed. Although the available records and information are limited, two major genetic lineages of ZIKV were identified in previous studies. However, in this study, three lineages were identified based on a phylogenetic analysis of all virus sequences from GenBank, including those of the envelope protein (E) and non-structural protein 5 (NS5) coding regions. The spatial and temporal distributions of the three identified ZIKV lineages and the recombination events and mechanisms underlying their divergence and evolution were further elaborated. The potential migration pathway of ZIKV was also characterized. Our findings revealed the central roles of two African countries, Senegal and Cote d'Ivoire, in ZIKV evolution and genotypic divergence. Furthermore, our results suggested that the outbreaks in Asia and the Pacific islands originated from Africa. The results provide insights into the geographic origins of ZIKV outbreaks and the spread of the virus, and also contribute to a better understanding of ZIKV evolution, which is important for the prevention and control of ZIKV infections.

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Genetic and Serologic Properties of Zika Virus Associated with an Epidemic, Yap State, Micronesia, 2007

Cited by 2,001 publications

References 19 publications

Zika virus and Guillain–Barré syndrome in Bangladesh

Zika virus and Guillain–Barré syndrome in Bangladesh

Rapid diagnosis of Zika virus through saliva and urine by Loop-mediated isothermal amplification (LAMP)

Phylogenetic analysis revealed the central roles of two African countries in the evolution and worldwide spread of Zika virus

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