2022
DOI: 10.1128/mbio.00374-22
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Genetic and Structural Variation in the O-Antigen of Salmonella enterica Serovar Typhimurium Isolates Causing Bloodstream Infections in the Democratic Republic of the Congo

Abstract: The bacterium Salmonella Typhimurium forms a devastating burden in sub-Saharan Africa by causing invasive bloodstream infections. Additionally, Salmonella Typhimurium presents high levels of antimicrobial resistance, jeopardizing treatment.

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Cited by 6 publications
(4 citation statements)
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“…The first protocol examines two genes, fliC and fljB, which play a role in flagella production [ 18 ]. The second protocol focuses on genes in the rfb locus, which is responsible for the production of O-polysaccharides [ 19 ]. From a genetic standpoint, the rfb locus is approximately 8-23 kb in length, making it challenging to amplify using a conventional PCR protocol.…”
Section: Discussionmentioning
confidence: 99%
“…The first protocol examines two genes, fliC and fljB, which play a role in flagella production [ 18 ]. The second protocol focuses on genes in the rfb locus, which is responsible for the production of O-polysaccharides [ 19 ]. From a genetic standpoint, the rfb locus is approximately 8-23 kb in length, making it challenging to amplify using a conventional PCR protocol.…”
Section: Discussionmentioning
confidence: 99%
“…In the Democratic Republic of the Congo, the emergence of a Salmonella Typhimurium O:5-negative variant has been reported, a strain lacking O:5 specificity [ 33 , 34 ]. This O:5-negative variant represented 37% of all Salmonella Typhimurium in the study by Tack et al For vaccine products based on O:4 antigens, such as the Salmonella glycoconjugate vaccine, the emergence of this O:5-negative variant may not influence their potential coverage.…”
Section: Discussionmentioning
confidence: 99%
“…Typhimurium overview in 2012, and including the ST313 lineage II D23580 isolate, used here as reference genome 38 . An additional 815 (57.4%) isolates were sequenced as part of the studies with isolates from sSA from Van Puyvelde et al 10 , 39 , Post et al 40 , Kariuki et al 11 , 13 , 41 , Feasey et al 42 , Msefula et al 43 , Park et al 14 and Maclennan et al (in preparation). As part of this study, 372 (26.2%) additional isolates from sSA were whole genome sequenced.…”
Section: Methodsmentioning
confidence: 99%