Genetic architecture of resistance in Daphnia hosts against two species of host-specific parasites

Routtu, Jarkko; Ebert, Dieter

doi:10.1038/hdy.2014.97

Cited by 57 publications

(104 citation statements)

References 58 publications

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“…Supporting the association mapping, the region on scaffold 626_944 matches with a genomic region previously identified by a QTL analysis of resistance to P. ramosa and which explained 50% of the observed variation for this trait (scaffold 944 in Routtu and Ebert (2015) is part of the super scaffold 626_944). In addition, this region displays a striking abundance of four functional categories involved in immune response or non-self recognitionfor example, chitinases (Lee et al 2011;Nance et al 2012;Beckerman et al 2013), serine proteases (Jang et al 2008), fucosyltransferases (Ma et al 2006;Davies 2009;Kashiwazaki et al 2014), and lactosylceramides (Iwabuchi et al 2012).…”

Section: Discussionsupporting

confidence: 71%

“…Here, we use such a combined approach to test whether selection by a parasite, the bacterium Pasteuria ramosa, is a dominant factor in the molecular evolution of its host, the planktonic crustacean Daphnia magna. As a previous QTL study pinpointed a genomic region in D. magna strongly associated with resistance to P. ramosa (Routtu and Ebert 2015), we hypothesized that selection at this locus or other genomic regions associated with resistance constitutes a major evolutionary factor in natural D. magna populations. A strict correspondence between P. ramosa and D. magna genotypes is required for the parasite to enter and infect its host, following a matching-allele model .…”

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confidence: 99%

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Parasitism drives host genome evolution: Insights from thePasteuria ramosa-Daphnia magnasystem

et al. 2017

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Because parasitism is thought to play a major role in shaping host genomes, it has been predicted that genomic regions associated with resistance to parasites should stand out in genome scans, revealing signals of selection above the genomic background. To test whether parasitism is indeed such a major factor in host evolution and to better understand host-parasite interaction at the molecular level, we studied genome-wide polymorphisms in 97 genotypes of the planktonic crustacean Daphnia magna originating from three localities across Europe. Daphnia magna is known to coevolve with the bacterial pathogen Pasteuria ramosa for which host genotypes (clonal lines) are either resistant or susceptible. Using association mapping, we identified two genomic regions involved in resistance to P. ramosa, one of which was already known from a previous QTL analysis. We then performed a naïve genome scan to test for signatures of positive selection and found that the two regions identified with the association mapping further stood out as outliers. Several other regions with evidence for selection were also found, but no link between these regions and phenotypic variation could be established. Our results are consistent with the hypothesis that parasitism is driving host genome evolution.

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Section: Discussionsupporting

confidence: 71%

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confidence: 99%

Parasitism drives host genome evolution: Insights from thePasteuria ramosa-Daphnia magnasystem

et al. 2017

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“…The latter analysis might also reveal signs of selection in these regions and, possibly, the nature of the selective pressures acting on browsing behaviour. No colocalization with loci identified in other genetic mapping analyses for D. magna (Routtu et al ., , ; Roulin et al ., ; Routtu & Ebert, ) was found for the browsing behaviour QTLs.…”

Section: Discussionmentioning

confidence: 99%

Ecological genetics of sediment browsing behaviour in a planktonic crustacean

Arbore

Andras

Routtu

et al. 2016

J of Evolutionary Biology

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Zooplankton can display complex habitat selection behaviours that influence the way they interact with their environments. Some species, although primarily pelagic, can exploit sediment-borne particles as a food source or use sediments as a refuge from pelagic predation. However, this strategy may increase the exposure to other risks such as benthic predation and infection from sediment-borne parasite transmission stages. The evolution of habitat selection behaviour in these species is thus expected to be influenced by multiple and possibly contrasting selective forces. Here, we study the browsing behaviour of the water flea Daphnia magna on bottom sediments. First, we demonstrated genetic variation for sediment browsing among D. magna genotypes from natural populations sampled across a broad geographic range. Next, we used an F2 recombinant panel to perform a QTL analysis and identified three regions in the D. magna genome contributing to variation in browsing behaviour. We also analysed the correlation between our data and previously published data on the phototactic behaviour of genotypes from the same F2 panel. Clonal means of the two behavioral traits were not correlated, suggesting that they may evolve independently. Browsing behaviour is likely to be a relevant component of habitat selection in D. magna, and its study may help to incorporate the interactions with the sediment into eco-evolutionary models of this key freshwater species.

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“…; Roulin et al . ; Routtu & Ebert ). One clone (from Southern Germany; DE‐Iinb1) is the result of one round of selfing, and the other clone (from Finland, FI‐Xinb3) resulted from three rounds of selfing.…”

Section: Methodsmentioning

confidence: 99%

Genes mirror geography in Daphnia magna

et al. 2015

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Identifying the presence and magnitude of population genetic structure remains a major consideration in evolutionary biology as doing so allows one to understand the demographic history of a species as well as make predictions of how the evolutionary process will proceed. Next-generation sequencing methods allow us to reconsider previous ideas and conclusions concerning the distribution of genetic variation, and what this distribution implies about a given species evolutionary history. A previous phylogeographic study of the crustacean Daphnia magna suggested that, despite strong genetic differentiation among populations at a local scale, the species shows only moderate genetic structure across its European range, with a spatially patchy occurrence of individual lineages. We apply RAD sequencing to a sample of D. magna collected across a wide swath of the species' Eurasian range and analyse the data using principle component analysis (PCA) of genetic variation and Procrustes analytical approaches, to quantify spatial genetic structure. We find remarkable consistency between the first two PCA axes and the geographic coordinates of individual sampling points, suggesting that, on a continent-wide scale, genetic differentiation is driven to a large extent by geographic distance. The observed pattern is consistent with unimpeded (i.e. no barriers, landscape or otherwise) migration at large spatial scales, despite the fragmented and patchy nature of favourable habitats at local scales. With high-resolution genetic data similar patterns may be uncovered for other species with wide geographic distributions, allowing an increased understanding of how genetic drift and selection have shaped their evolutionary history.

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Genetic architecture of resistance in Daphnia hosts against two species of host-specific parasites

Cited by 57 publications

References 58 publications

Parasitism drives host genome evolution: Insights from thePasteuria ramosa-Daphnia magnasystem

Parasitism drives host genome evolution: Insights from thePasteuria ramosa-Daphnia magnasystem

Ecological genetics of sediment browsing behaviour in a planktonic crustacean

Genes mirror geography in Daphnia magna

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