Genetic aspects of the tolerance to allografts induced at metamorphosis in the toadXenopus laevis

Pasquier, Louis Du; Chardonnens, X

doi:10.1007/bf01572313

Cited by 50 publications

(25 citation statements)

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“…In Xenopus, as in mammals, acute skin rejection is associated with MHC incompatibility (5)(6)(7)(8). In contrast, a slower (i.e., chronic) rejection occurs when only minor H-ags are involved (9).…”

Section: Discussionmentioning

confidence: 96%

“…rochester.edu/smd/mbi/xenopus/index.htm); all animals were handled under strict laboratory and University Committee on Animal Resources (UCAR) regulations, minimizing discomfort at all times. LG-6 and LG-15 clones were originally typed by mixed lymphocyte reaction and skin graft (6,7). Progeny from these clones were generated in our lab by gynogenesis (18) from females typed by skin graft.…”

Section: Animalsmentioning

confidence: 99%

“…However, the Xenopus MHC encodes for only one class Ia gene (4). At the functional level, adult Xenopus can recognize and reject skin allografts from donors that differ either by MHC plus minor histocompatibility (H)-antigen (5)(6)(7)(8) or only by minor histocompatibility (H)-antigens (ags) (9,10). Several different MHC-defined clones of Xenopus permit the study of specific immunity in isogenetic MHCdefined animals.…”

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confidence: 99%

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In Vivo Study of T-Cell Responses to Skin Alloantigens in Xenopus Using a Novel Whole-Mount Immunohistology Method

et al. 2007

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Section: Discussionmentioning

confidence: 96%

Section: Animalsmentioning

confidence: 99%

mentioning

confidence: 99%

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In Vivo Study of T-Cell Responses to Skin Alloantigens in Xenopus Using a Novel Whole-Mount Immunohistology Method

et al. 2007

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“…Thus, Xenopus MLR, CML, acute graft rejection, lymphocyte and erythrocyte alloantigens, and effective T-B cell cooperation were found to segregate together in the progeny of outbred parents as if they were controlled by the same genetic locus, or by a cluster of tightly linked loci [ 15,21,[26][27][28]. Therefore, genetic studies of MLR, graft rejection and CML in the snake I!…”

Section: Linkage Of Functional Markersmentioning

confidence: 99%

Functional markers of the major histocompatibility gene complex of snakes

Farag

Ridi

1990

Eur J Immunol

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In optimal seasonal conditions, outbred adult snakes Psammophis sibilans displayed the major immunological functions related, in mammals, to the presence of the major histocompatibility gene complex (MHC). Thus, out of 30 snake random pairs that exchanged skin transplants 72.9% rejected their allograft in an acute or subacute manner. Strongly significant proliferative response was recorded in 67.3% of 168 separate one-way mixed leukocyte reaction (MLR) cultures. Lymphocytes from 6/11 snakes immunized by skin allografting displayed, after secondary stimulation in vitro, cell-mediated lympholysis (CML) in vitro of 51Cr-labeled lymphoblasts derived from the donor snake. Finally, cytotoxic alloantibodies were readily generated after snake priming with skin allograft and blood cells. Snakes did not only exhibit the major cell- and humoral-mediated immune functions, but these functions appeared to be linked with the degree of MLR disparity. Thus, animals with different MLR rejected skin allografts acutely and produced cytotoxic effector cells. In contrast, MLR-identical animals rejected the skin allograft of their partner chronically and failed to produce killer cells in CML. This significant positive correlation between MLR disparity, graft rejection and CML suggests that the responsible antigens are encoded, as in other vertebrates, by the same genetic system, the MHC.

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“…A major attribute of the X. laevis model is the availability of different MHC-defined strains and clones. In addition to the MHC homozygous inbred strains, the J and F strains (Du Pasquier and Chardonnens 1975;Tochinai and Katagiri 1975), the X. laevis model also includes MHC-defined isogenic clones of frogs, such as the LG-15 and LG-6 that share the same heterozygous MHC haplotypes (a/c) but differ at multiple minor histocompatability (H) loci (Kobel and Du Pasquier 1975;Kobel and Du Pasquier 1977). These clones are generated by gynogenesis, a procedure where diploid eggs from X. laevis/X.…”

Section: The Immune System Of Xenopus Laevismentioning

confidence: 99%

Comparative Study of Skin Graft Tolerance and Rejection in the Frog Xenopus Laevis

Nedelkovska¹,

Robert²

2011

Skin Grafts - Indications, Applications and Current Research

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Genetic aspects of the tolerance to allografts induced at metamorphosis in the toadXenopus laevis

Cited by 50 publications

References 7 publications

In Vivo Study of T-Cell Responses to Skin Alloantigens in Xenopus Using a Novel Whole-Mount Immunohistology Method

In Vivo Study of T-Cell Responses to Skin Alloantigens in Xenopus Using a Novel Whole-Mount Immunohistology Method

Functional markers of the major histocompatibility gene complex of snakes

Comparative Study of Skin Graft Tolerance and Rejection in the Frog Xenopus Laevis

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