Genetic assessment of Abies koreana (Pinaceae), the endangered Korean fir, and conservation implications

Kwak, Myounghai; Hong, Jeong-Ki; Park, Jin Hee; Lee, Byoung Yoon; Suh, Min Hwan; Kim, Chan Soo

doi:10.1007/s10592-017-0968-0

Cited by 13 publications

(14 citation statements)

References 59 publications

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“…The dried leaves were ground in a bead mill (Tissue Lyser II; Qiagen, Hilden, Germany) and total genomic DNA was extracted using a DNeasy Plant Mini Kit (Qiagen). We amplified 19 microsatellite loci from specimens as described previously for wild A. koreana populations (Kwak et al, 2017). Two to four microsatellite primer pairs were amplified in each multiplex reaction using the Qiagen Multiplex polymerase chain reaction (PCR) Kit, according to the manufacturer's instructions.…”

Section: Methodsmentioning

confidence: 99%

“…Population genetic diversity measures were calculated using GenAlEx version 6.4.1 (Peakall and Smouse, 2006) and compared with the results of wild populations (Kwak et al, 2017). Allelic richness was calculated assuming a sample size of 20 individuals using a rarefaction approach in ADZE (Szpiech et al, 2008).…”

Section: Methodsmentioning

confidence: 99%

“…Allelic richness was calculated assuming a sample size of 20 individuals using a rarefaction approach in ADZE (Szpiech et al, 2008). To investigate the genetic characteristics of A. koreana cultivars, further analysis was performed on the combined data from a total of 239 individuals, including the wild A. koreana described previously (Kwak et al, 2017). The shared allele distance [D SA (Chakraborty and Jin, 1993)] was calculated using PowerMarker version 3.0 (Liu and Muse, 2005).…”

Section: Methodsmentioning

confidence: 99%

“…Sokrisan (SR) ( Fig. 1) (Kwak et al, 2017). The genetic diversity of the HL cluster is the lowest Fig.…”

mentioning

confidence: 98%

“…Korean fir (A. koreana) is a popular ornamental conifer planted in cold-climate gardens and preferred as Christmas trees in North American and European countries (Cregg, 2008). This species is endemic to Korea, where it is currently distributed in the southern part of the Korean peninsula and on Jejudo Island, South Korea (Kwak et al, 2017) (Fig. 1).…”

mentioning

confidence: 99%

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Evaluation of Genetic Diversity in Korean Fir Cultivars Based on Microsatellite and Cytoplasmic Markers

Kwak¹,

Hong²,

Lee³

et al. 2020

J. Amer. Soc. Hort. Sci.

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Korean fir (Abies koreana) has been cultivated for less than 100 years, mainly in the United States and Europe. Using nuclear microsatellite, mitochondrial, and chloroplast markers, we investigated the origin of cultivated korean fir from South Korea (KC group) as well as the United States and United Kingdom (EU group), and compared these samples to published data from wild populations. All genotypes in the EU and KC groups were most closely related to the wild individuals from Mt. Hallasan, the southernmost A. koreana population on Jejudo Island (South Korea). However, the presence of the chloroplast haplotypes clustered with Abies balsamea in two EU cultivars and the higher diversity values of the EU group compared with the wild individuals from Mt. Hallasan infer a certain level of introgression from different species during cultivation. The EU group had a higher inbreeding coefficient and linkage disequilibrium, and a smaller proportion of rare alleles, than the wild populations. This suggests that the genetic characteristics of korean fir cultivars reflect strong artificial selection pressure for desirable horticultural traits and asexual reproduction. Last, this genetic background study suggests that the other wild populations in the Korean peninsula can serve as valuable genetic resources for future breeding.

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Section: Methodsmentioning

confidence: 99%