Genetic Association of Bovine Lymphocyte Antigen DRB3 Alleles with Immunological Traits of Holstein Cattle

Abstract

The associations between alleles at the BoLA (bovine lymphocyte antigen) DRB3 locus and 20 indicator traits of innate and adaptive immunity were investigated. Periparturient Holsteins (n = 127) were genotyped at the BoLA DRB3 locus using polymerase chain reaction and restriction fragment length polymorphism. Twenty-two alleles were observed in the study population, and frequencies ranged from 21 to < 1%. The same cattle were tested for a total of 20 innate and adaptive immunity traits, including lymphocyte response to mitogens (proliferative responses and Ig secretion), serum Ig, complement and conglutinin concentrations, total leukocyte count, and selected assays for neutrophil function. Models with gene substitution effects were used to investigate associations between BoLA DRB3 alleles and each of the immunological variables. Significant associations were found with 13 of the leukocyte functions tested. The number of immune parameters with significant associations with any allele ranged from 0 (with alleles DRB3.2*23 and DRB3.2*27) to 7 (with DRB3.2*8). The immunological parameter that had the most associations with alleles was serum IgG2 concentration with 6 alleles. One group of 4 alleles (representing 46% of the total allele frequency) was uniformly associated with increased IgM and complement and decreased mononuclear cell numbers. Thus, we demonstrated that the BoLA DRB3 genotype can influence measures of innate and adaptive immunity.

A novel association of BoLA DRB3 alleles in BLV infected cattle with different proviral loads

“…On the other hand, other issues like production traits improvement, resistance to mastitis pathogens, somatic cell counts and milk yield have also been related to specific BoLA alleles (DIETZ et al, 1997;RUPP et al, 2007;YOSHIDA et al, 2009;BALTIAN et al, 2012;YOSHIDA et al, 2012). However, some controversies exist between different studies.…”

“…These genes are associated with genetic resistance and susceptibility to a wide range of diseases, including mastitis (DIETZ et al, 1997;RUPP et al, 2007;YOSHIDA et al, 2009;YOSHIDA et al, 2012), dermatophilosis (MAILLARD et al, 2002), tick-borne diseases (DUANGJINDA et al, 2013), infection with Neospora caninum (SCHWAB et al, 2009), African trypanosome species (KARIMURIBO et al, 2011), and Theileria annulata (GLASS et al, 2012b). Moreover, BoLA appears to influence other traits such as milk yield, growth and reproduction, and variations in immune responses to antigens (TAKESHIMA; AIDA, 2006) and even vaccine peptides (GARCIA-BRIONES et al, 2000;GLASS et al, 2012a).…”

Descobrindo genes expressos na glândula mamária e relacionados à ocorrência e controle da mastite bovina

“…No entanto, a resistência a doenças apresenta baixa herdabilidade (Shook, 1989), sendo reduzidos os ganhos genéticos para essa característica. Em razão disso, vários autores têm realizado estudos empregando técnicas de genética molecular em estudos de saúde da glândula mamária dos bovinos leiteiros (Bernoco & Lewin, 1989;Dietz et al, 1997a;Dietz et al, 1997b;Groenen et al, 1990;Giovambatista et al, 1996;Lewin, 1989;Østergärd et al, 1989;Starkenburg et al, 1997) O aumento nas características de produção dos animais apresenta vantagens evidentes tanto para produtores quanto para consumidores. A produção animal representa mais da metade da renda proveniente do setor agrícola nos países desenvolvidos e mais de um quarto nos países em desenvolvimento.…”

“…Há genes descritos e envolvidos na biologia de resistência a doenças (Dietz et al, 1997a;Seyfert et al, 1996) podem influenciar a produção subseqüente de anticorpos (Østergärd et al, 1989 (Burke et al, 1991;Russell et al, 1994). Como o gene BoLA-DRB3 é altamente expresso (Burke et al, 1991;Fraser et al, 1996), estudos têm sido realizados (Dietz et al, 1997a;Dietz et al, 1997b;Giovambatista et al, 1996;Starkenburg et al, 1997) Trata-se, portanto de um recurso importante para estudos de expressão gênica e seu emprego neste estudo está detalhado no Capítulo 7.…”

“…Como o gene BoLA-DRB3 é altamente expresso (Burke et al, 1991;Fraser et al, 1996), estudos têm sido realizados (Dietz et al, 1997a;Dietz et al, 1997b;Giovambatista et al, 1996;Starkenburg et al, 1997) Trata-se, portanto de um recurso importante para estudos de expressão gênica e seu emprego neste estudo está detalhado no Capítulo 7.…”