Genetic background of host—pathogen interaction betweenCucumis sativus L. andPseudomonas syringae pv.lachrymans

Olczak-Woltman, Helena; Schollenberger, M.; Niemirowicz-Szczytt, K.

doi:10.1007/bf03195645

Cited by 11 publications

(4 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As the interaction between salinity and pathogens is still poorly recognized, we discuss here how salt stress influences the plant response to diseases, taking leads from studies on cucumber sequentially exposed to salt stress and angular leaf spot (ALS), the second most severe cucumber disease, caused by Psl ( Olczak-Woltman et al, 2009 ).…”

Section: Case Study: Cucumber Response To Sequential Salt Stress and mentioning

confidence: 99%

The Effects of Combined Abiotic and Pathogen Stress in Plants: Insights From Salinity and Pseudomonas syringae pv lachrymans Interaction in Cucumber

2018

View full text Add to dashboard Cite

Plants are often challenged by abiotic and biotic stresses acting in combination and the response to combinatorial stress differs from that triggered by each factor individually. Although salinity and pathogens are major stressors limiting plant growth and productivity worldwide, their interaction is poorly understood. The reactions to pathogens overlap with those to abiotic stresses, and reactive oxygen species (ROS) and stress hormones represent central nodes in the interacting signaling pathways. Usually, abiotic stress negatively affects plant susceptibility to disease. Specific focus of this review is on cucumber plants exposed to salt stress and thereafter infected with Pseudomonas syringae pv lachrymans (Psl). We addressed this problem by discussing the changes in photochemistry, the antioxidant system, primary carbon metabolism, salicylic acid (SA) and abscisic acid (ABA) contents. Salt-treated plants were more prone to infection and this effect was determined by changes in the hormonal and redox balance as well as the carboxylate metabolism and activities of some NADPH-generating enzymes. Our detailed understanding of the interactive effects of biotic and abiotic stresses is fundamental to achieve enhanced tolerance to combination stress in agronomically important crops.

show abstract

Section: Case Study: Cucumber Response To Sequential Salt Stress and mentioning

confidence: 99%

The Effects of Combined Abiotic and Pathogen Stress in Plants: Insights From Salinity and Pseudomonas syringae pv lachrymans Interaction in Cucumber

2018

View full text Add to dashboard Cite

show abstract

“…Cucumbers are commonly cultivated in greenhouses due to their preference for warm temperatures. Because of the high humidity in greenhouses, they frequently encounter many different types of pathogens, including bacterial, viral, fungal, and oomycete, that severely prevent growth and may have a great impact on production [ 31 , 32 , 33 ]. Of these, Botrytis cinerea is the causal agent of gray mold, which is one of the top 10 fungal plant pathogens and causes severe damage, both pre- and post-harvest.…”

Section: Introductionmentioning

confidence: 99%

Pan-Genome Analysis of TIFY Gene Family and Functional Analysis of CsTIFY Genes in Cucumber

Liu,

Xu,

Gao

et al. 2023

IJMS

View full text Add to dashboard Cite

Cucumbers are frequently affected by gray mold pathogen Botrytis cinerea, a pathogen that causes inhibited growth and reduced yield. Jasmonic acid (JA) plays a primary role in plant responses to biotic stresses, and the jasmonate-ZIM-Domain (JAZ) proteins are key regulators of the JA signaling pathway. In this study, we used the pan-genome of twelve cucumber varieties to identify cucumber TIFY genes. Our findings revealed that two CsTIFY genes were present in all twelve cucumber varieties and showed no differences in protein sequence, gene structure, and motif composition. This suggests their evolutionary conservation across different cucumber varieties and implies that they may play a crucial role in cucumber growth. On the other hand, the other fourteen CsTIFY genes exhibited variations in protein sequence and gene structure or conserved motifs, which could be the result of divergent evolution, as these genes adapt to different cultivation and environmental conditions. Analysis of the expression profiles of the CsTIFY genes showed differential regulation by B. cinerea. Transient transfection plants overexpressing CsJAZ2, CsJAZ6, or CsZML2 were found to be more susceptible to B. cinerea infection compared to control plants. Furthermore, these plants infected by the pathogen showed lower levels of the enzymatic activities of POD, SOD and CAT. Importantly, after B. cinerea infection, the content of JA was upregulated in the plants, and cucumber cotyledons pretreated with exogenous MeJA displayed increased resistance to B. cinerea infection compared to those pretreated with water. Therefore, this study explored key TIFY genes in the regulation of cucumber growth and adaptability to different cultivation environments based on bioinformatics analysis and demonstrated that CsJAZs negatively regulate cucumber disease resistance to gray mold via multiple signaling pathways.

show abstract

“…lachrymans ), viral (e.g., Cucumber mosaic virus ), fungal (e.g., Sphaerotheca fulginea and Erysiphe cichoracearum ), and oomycete (e.g., Phytophthora capsici and Pseudoperonospora cubensis ) pathogens [10], [11]. Of these, the most destructive is Ps.…”

Section: Introductionmentioning

confidence: 99%

Expression Profiling of Cucumis sativus in Response to Infection by Pseudoperonospora cubensis

et al. 2012

View full text Add to dashboard Cite

The oomycete pathogen, Pseudoperonospora cubensis, is the causal agent of downy mildew on cucurbits, and at present, no effective resistance to this pathogen is available in cultivated cucumber (Cucumis sativus). To better understand the host response to a virulent pathogen, we performed expression profiling throughout a time course of a compatible interaction using whole transcriptome sequencing. As described herein, we were able to detect the expression of 15,286 cucumber genes, of which 14,476 were expressed throughout the infection process from 1 day post-inoculation (dpi) to 8 dpi. A large number of genes, 1,612 to 3,286, were differentially expressed in pair-wise comparisons between time points. We observed the rapid induction of key defense related genes, including catalases, chitinases, lipoxygenases, peroxidases, and protease inhibitors within 1 dpi, suggesting detection of the pathogen by the host. Co-expression network analyses revealed transcriptional networks with distinct patterns of expression including down-regulation at 2 dpi of known defense response genes suggesting coordinated suppression of host responses by the pathogen. Comparative analyses of cucumber gene expression patterns with that of orthologous Arabidopsis thaliana genes following challenge with Hyaloperonospora arabidopsidis revealed correlated expression patterns of single copy orthologs suggesting that these two dicot hosts have similar transcriptional responses to related pathogens. In total, the work described herein presents an in-depth analysis of the interplay between host susceptibility and pathogen virulence in an agriculturally important pathosystem.

show abstract

Genetic background of host—pathogen interaction betweenCucumis sativus L. andPseudomonas syringae pv.lachrymans

Cited by 11 publications

References 36 publications

The Effects of Combined Abiotic and Pathogen Stress in Plants: Insights From Salinity and Pseudomonas syringae pv lachrymans Interaction in Cucumber

The Effects of Combined Abiotic and Pathogen Stress in Plants: Insights From Salinity and Pseudomonas syringae pv lachrymans Interaction in Cucumber

Pan-Genome Analysis of TIFY Gene Family and Functional Analysis of CsTIFY Genes in Cucumber

Expression Profiling of Cucumis sativus in Response to Infection by Pseudoperonospora cubensis

Contact Info

Product

Resources

About