Genetic Basis of Erythromycin Resistance in Oral Bacteria

Villedieu, A.; Diaz-Torres, Martha; Roberts, Adam P.; Hunt, NP; McNab, Rod; Spratt, DA; Wilson, Michael; Mullany, Peter

doi:10.1128/aac.48.6.2298-2301.2004

Cited by 39 publications

(26 citation statements)

References 32 publications

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“…The high prevalence of TET resistance in oral E. faecalis could be the result of the promiscuous spread of tet(M) containing Tn916-related elements within oral microbiota, while our data do not allow further speculations on this. To sum up, our observations are consistent with the general recognition of oral microflora as an important reservoir for antimicrobial resistance (30,42,43).…”

Section: Discussionsupporting

confidence: 90%

Occurrence, Population Structure, and Antimicrobial Resistance of Enterococci in Marginal and Apical Periodontitis

et al. 2009

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Subgingival plaque samples and root canal samples were collected from 2,839 marginal periodontitis (MP) patients and 21 apical periodontitis (AP) patients. Enterococcus species were identified by a series of phenotypic and genotypic tests. Antimicrobial susceptibility assays were performed by an agar disk diffusion test. Multilocus sequence typing (MLST), eBURST, and minimum spanning tree were used for enterococcal genetic clustering and population analysis. Enterococcus faecalis was recovered from 3.7% MP patients and 9.5% AP patients, and Enterococcus faecium was recovered from 0.04% MP patients. Enterococci were detected more often in older male patients. E. faecalis isolates of MP were found resistant to tetracycline (49.1%), erythromycin (8.5%), trimethoprim (2.8%), and gentamicin (1.9%), while one AP isolate was resistant to tetracycline. A total of 40 sequence types (STs) were resolved in 108 E. faecalis isolates. Comparison with E. faecalis international MLST database revealed that 27 STs were previously found, 13 STs were novel, and several major clonal complexes in the database were also found in MP isolates. The tetracycline-resistant isolates distributed mainly in the major clonal complexes and singletons, whereas the erythromycin-resistant isolates were more dispersed. Although the rate of occurrence of enterococci recovered in the MP and AP samples was low, 50% of these isolates are resistant to at least one antimicrobial agent, which is most often tetracycline. This implies that subgingival E. faecalis might represent a reservoir of resistance to tetracycline and erythromycin. The subgingival E. faecalis isolates show high genetic diversity but are grouped, in general, with the known isolates from the international database.

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Section: Discussionsupporting

confidence: 90%

Occurrence, Population Structure, and Antimicrobial Resistance of Enterococci in Marginal and Apical Periodontitis

et al. 2009

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“…Differences in restriction patterns of mobile ele- ments have been reported previously, e.g. with Tn1545 elements [13]. The amplified products of int 916 , int 5252 , umuC and umuD from these five isolates were sequenced (sequences listed under accession nos.…”

Section: Forward Primermentioning

confidence: 98%

Diversity of putative Tn5253-like elements in Streptococcus pneumoniae

Henderson-Begg

Roberts

Hall

2009

International Journal of Antimicrobial Agents

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“…The oral microflora is an important reservoir for transferable antimicrobial resistance [1], [2], [3], [4]. Our previous study of oral Enterococcus faecalis isolated from Norwegian patients with marginal and apical periodontitis showed that approximately 50% of the strains were resistant to one or more of the tested antimicrobial agents, mostly tetracycline and/or erythromycin [5], [6].…”

Section: Introductionmentioning

confidence: 99%

Characterisation of the Plasmidome within Enterococcus faecalis Isolated from Marginal Periodontitis Patients in Norway

et al. 2013

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The present study aimed to identify and characterize plasmids in a national collection of oral Enterococcus faecalis (n = 106) isolated from patients with marginal periodontitis. Plasmid replicon typing was performed by multiplex-PCR and sequencing with specific primers for 18 rep-families and 1 unique sequence. Additional plasmid analysis by S1-PFGE was performed for comparison. Totally 120 plasmid replicon amplicons of seven rep-families were identified in 93 E. faecalis strains, e.g. rep9 (prototype pCF10), rep6 (prototype pS86), rep2 (prototype pRE25/pEF1), and rep8 (prototype pAM373). Rep9 was the most predominant rep-family being detected in 81 (76.4%) strains. Forty of these strains were tetracycline resistant and three were erythromycin resistant. Rep6 was the second predominant rep-family being detected in 22 (20.8%) strains. Rep2 was detected in eight (7.5%) strains. All rep2-positive strains were resistant to tetracycline and/or erythromycin and six of them contained Tn916/Tn1545 genes. The rep-positive E. faecalis exhibited divergence in multilocus sequence types (STs). There was a significant correlation between rep9 and ST21, while multiple rep-families appeared in ST40. Totally 145 plasmid bands were identified in 95 E. faecalis strains by S1-PFGE, 59 strains carrying one plasmid, 27 carrying two, five carrying three, three carrying four, and one strain carrying five plasmids. Plasmid sizes varied between 5–150 kbp. There was a significant correlation between the number of plasmids identified by PCR rep-typing and by S1-PFGE. The results indicate that the majority of E. faecalis of marginal periodontitis are likely to be a reservoir for diverse mobile genetic elements and associated antimicrobial resistance determinants.

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Genetic Basis of Erythromycin Resistance in Oral Bacteria

Cited by 39 publications

References 32 publications

Occurrence, Population Structure, and Antimicrobial Resistance of Enterococci in Marginal and Apical Periodontitis

Occurrence, Population Structure, and Antimicrobial Resistance of Enterococci in Marginal and Apical Periodontitis

Diversity of putative Tn5253-like elements in Streptococcus pneumoniae

Characterisation of the Plasmidome within Enterococcus faecalis Isolated from Marginal Periodontitis Patients in Norway

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