Genetic Basis of Resistances to Chlorfenapyr and Etoxazole in the Two-Spotted Spider Mite (Acari: Tetranychidae)

Uesugi, Ryuji; Goka, Kouichi; Osakabe, Masahiro

doi:10.1603/0022-0493-95.6.1267

Cited by 67 publications

(44 citation statements)

References 16 publications

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“…To determine the molecular action of etoxazole, we first characterized an etoxazole-resistant T. urticae strain from Japan (strain EtoxR), where etoxazole has been in use for more than a decade, and field resistance has been reported (13). In comparison with the etoxazole-susceptible London strain, EtoxR had a resistance ratio of 48,000-fold ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Population bulk segregant mapping uncovers resistance mutations and the mode of action of a chitin synthesis inhibitor in arthropods

Leeuwen

Demaeght

Osborne

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

250

291

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Because of its importance to the arthropod exoskeleton, chitin biogenesis is an attractive target for pest control. This point is demonstrated by the economically important benzoylurea compounds that are in wide use as highly specific agents to control insect populations. Nevertheless, the target sites of compounds that inhibit chitin biogenesis have remained elusive, likely preventing the full exploitation of the underlying mode of action in pest management. Here, we show that the acaricide etoxazole inhibits chitin biogenesis in Tetranychus urticae (the two-spotted spider mite), an economically important pest. We then developed a populationlevel bulk segregant mapping method, based on high-throughput genome sequencing, to identify a locus for monogenic, recessive resistance to etoxazole in a field-collected population. As supported by additional genetic studies, including sequencing across multiple resistant strains and genetic complementation tests, we associated a nonsynonymous mutation in the major T. urticae chitin synthase (CHS1) with resistance. The change is in a C-terminal transmembrane domain of CHS1 in a highly conserved region that may serve a noncatalytic but essential function. Our finding of a target-site resistance mutation in CHS1 shows that at least one highly specific chitin biosynthesis inhibitor acts directly to inhibit chitin synthase. Our work also raises the possibility that other chitin biogenesis inhibitors, such as the benzoylurea compounds, may also act by inhibition of chitin synthases. More generally, our genetic mapping approach should be powerful for high-resolution mapping of simple traits (resistance or otherwise) in arthropods.

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Section: Resultsmentioning

confidence: 99%

Population bulk segregant mapping uncovers resistance mutations and the mode of action of a chitin synthesis inhibitor in arthropods

Leeuwen

Demaeght

Osborne

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

250

291

View full text Add to dashboard Cite

show abstract

“…The modes of inheritance of resistance to various pesticides have been studied in many pest species, including insects (Payne et al 1988;Heim et al 1992;Huang et al 1999;Daborn et al 2000;Bouvier et al 2001), spider mites (Rizzieri et al 1988;Goka 1998;Uesugi et al 2002), and ticks (Lourens 1979(Lourens , 1980. The modes of resistance to organochlorine and organophosphate acaricides have been well documented in B. microplus from early studies in Australia (Stone 1962;Wilson et al 1971;Stone et al 1973;Stone and Youlton 1982).…”

Section: Discussionmentioning

confidence: 99%

Mode of Inheritance of Amitraz Resistance in a Brazilian Strain of the Southern Cattle Tick, Boophilus microplus (Acari: Ixodidae)*

Davey

Miller

et al. 2005

Exp Appl Acarol

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The southern cattle tick, Boophilus microplus (Canestrini), has developed resistance to amitraz in several countries in recent years. A study was conducted at the USDA Cattle Fever Tick Research Laboratory in Texas to investigate the mode of inheritance of amitraz resistance with cross-mating experiments. The Muñoz strain, a laboratory reared acaricide-susceptible reference strain, was used as the susceptible parent and the Santa Luiza strain, originating in Brazil, was used as the resistant parent. A modified Food and Agriculture Organization Larval Packet Test was used to measure the levels of susceptibility of larvae of the parental strains, F1, backcross, F2, and F3 generations. Results of reciprocal crossing experiments suggested that amitraz resistance was inherited as an incomplete recessive trait. There was a strong maternal effect on larval progeny's susceptibility to amitraz in both the F1 and the subsequent generations. The values of the degree of dominance were estimated at -0.156 and -0.500 for the F1 larvae with resistant and susceptible female parents, respectively. Results of bioassays on larval progeny of the F1 backcrossed with the resistant parent strain and that of the F2 generations suggested that more than one gene was responsible for amitraz resistance in the Santa Luiza strain. Comparisons of biological parameters (engorged female weight, egg mass weight, and female-to-egg weight conversion efficiency index) indicated significant differences between different genotypes. The differences appeared to be heritable, but not related to amitraz resistance. Results from this study may have significant implications for the management of amitraz resistance.

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“…Although many acaricides with a different mode of action have been developed as a result of those properties of spider mites, a number of cross-resistances have been reported not only in the same chemical class of acaricides but also between compounds possessing different modes of action. 1,2) Therefore, it is desired to constantly discover and develop novel acaricides that will be effective against the population that is resistant to existing agrochemicals.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and biological activity of a novel acaricide, pyflubumide

Furuya

Suwa

Nakano

et al. 2015

Journal of Pesticide Science

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Pyflubumide is a novel acaricide with a unique chemical structure that contains a methoxy-substituted hexafluoroisopropyl group on the anilino moiety. The compound has shown remarkable activity against spider mites including strains resistant to existing acaricides collected from the fields of Japan. Since carboxin was developed in 1966, various carboxamides with the same mode of action, i.e., succinate dehydrogenase inhibitors, have been created and developed. Although succinate dehydrogenase plays an important role in energy metabolism in aerobic organisms, the practical usage of the carboxamides has been primarily limited to disease control and an acaricidal activity has never been reported. The study to create a new carboxamide molecule revealed that introducing a fluoroalkyl group at the 4′-position of the aniline molecule remarkably enhanced the acaricidal activity. This finding prompted extensive research to ultimately identify pyflubumide. In this report, details of the structure-activity relationships from the lead compound to pyflubumide are described.

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Genetic Basis of Resistances to Chlorfenapyr and Etoxazole in the Two-Spotted Spider Mite (Acari: Tetranychidae)

Cited by 67 publications

References 16 publications

Population bulk segregant mapping uncovers resistance mutations and the mode of action of a chitin synthesis inhibitor in arthropods

Population bulk segregant mapping uncovers resistance mutations and the mode of action of a chitin synthesis inhibitor in arthropods

Mode of Inheritance of Amitraz Resistance in a Brazilian Strain of the Southern Cattle Tick, Boophilus microplus (Acari: Ixodidae)*

Synthesis and biological activity of a novel acaricide, pyflubumide

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