Genetic basis of tetracycline resistance in clinical isolates of Listeria monocytogenes

Poyart-Salmeron, C.; Trieu-Cuot, Patrick; Carlier, C; MacGowan, A. P.; McLauchlin, James; Courvalin, Patrice

doi:10.1128/aac.36.2.463

Cited by 98 publications

(89 citation statements)

References 32 publications

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“…In previous studies, it has been proposed that emergence of multiple-antibiotic resistance in L. monocytogenes BM4210 was secondary to acquisition of a replicon originating in the Enterococcus-Streptococcus genera (22). Demonstration of a direct exchange of genetic information between Enterococcusfaecalis and L. monocytogenes in the digestive tract of gnotobiotic mice (8) and detection in L. monocytogenes of tet(M) associated with int-Tn, the gene encoding the protein required for the movements of enterococcal conjugative transposons, constitute additional support for this suggestion (25). The tet(S) gene was detected in two other clinical isolates of L. monocytogenes isolated in France and Switzerland (5) but its occurrence in enterococci and streptococci has not been reported.…”

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confidence: 85%

“…Total DNAs from Enterococcus and Streptococcus spp. were prepared as described previously (25). The presence of DNA was monitored by agarose gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

“…Genes tet(K) and tet(L) have been detected only in gram-positive bacteria (2,3,9,25,27,28,37). The tet(P) determinant, which is constituted of two overlapping genes, tetA(P) and tetB(P), has been found only in Clostridium spp.…”

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confidence: 99%

“…(29,31). Originally discovered in Streptococcus agalactiae (4), tet(M) has been subsequently detected in a large variety of aerobic and anaerobic gram-positive and gram-negative bacterial genera, including Enterococcus (2, 27, 37), Streptococcus (27,37), Peptostreptococcus (26,27), Staphylococcus (3), Listeria (9,25), Clostridium (11,19), Neisseria (16), Kingella (16), Gardnerella (27), Haemophilus (26), and Bacteroides (1), and in cell wall-less organisms such as Mycoplasma and Ureaplasma spp. (26).…”

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confidence: 99%

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Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis

Charpentier

Gerbaud

Courvalin

1994

Antimicrob Agents Chemother

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Two hundred thirty-eight tetracycline-and minocycline-resistant clinical isolates of Enterococcus and Streptococcus spp. were investigated by dot blot hybridization for the presence of nucleotide sequences related to tet(S) (first detected in Listeria monocytogenes BM4210), tet(K), tet(L), tet(M), tet(O), tet(P), and tet(Q) genes. The tet(S) determinant was found in 22 strains of Enterococcus faecalis, associated with tet(M) in 9 of these isolates and further associated with tet(L) in 3 of these strains. tet (M)

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confidence: 85%

“…Total DNAs from Enterococcus and Streptococcus spp. were prepared as described previously (25). The presence of DNA was monitored by agarose gel electrophoresis.…”

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis

Charpentier

Gerbaud

Courvalin

1994

Antimicrob Agents Chemother

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“…It appears that virtually the same profile has been identified over the years and in different countries. In relation to tetracycline, this research highlighted the extreme sensitivity of the 68 strains to this drug, contrasting with the emergence of clinical strains resistant to tetracycline, related to the gene tetM 5,22,23 .…”

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confidence: 99%

Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil

Reis

Barbosa

Rusak

et al. 2011

Rev. Soc. Bras. Med. Trop.

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INTRODUCTION: Listeria monocytogenes is the causative agent of listeriosis, a foodborne illness that affects mainly pregnant women, the elderly and immunocompromised patients. The primary treatment is a combination of ampicillin with an aminoglycoside, in addition to a second-choice drug represented by chloramphenicol, erythromycin, tetracycline and rifampicin. The aim of this study was to analyze the antimicrobial susceptibility profile of strains isolated from human sources in the last four decades. METHODS: Sixty-eight strains were selected from the culture collection of the Laboratory of Bacterial Zoonoses/LABZOO/FIOCRUZ isolated in different regions of Brazil from 1970 to 2008 and primarily isolated from cerebrospinal fluid and blood culture. Susceptibility tests to antimicrobials drugs were evaluated using the criteria established by Soussy using the Kirby-Bauer method and E-Test strips were used to determine the minimum inhibitory concentration (MIC). RESULTS: Among the strains tested, serovar L4b (60.3%) was the most prevalent, followed by serovar 1/2a (20.6%), 1/2b (13.2%) and the more uncommon serovars 1/2c, 3b and 4ab (5.9%). All strains were susceptible to ampicillin, cephalothin, erythromycin, gentamicin, teicoplanin and vancomycin. Only one strain (1.5%) showed resistance to rifampin, and two (3%) were resistant to trimethoprim-sulfamethoxazole. MICs with values up to 2μg/ml reinforce the need for microbiological surveillance. CONCLUSIONS: The study demonstrated low prevalence of strains resistant to the antimicrobial drugs indicated in the treatment of human listeriosis. Monitoring antimicrobial resistance profile is still very important to determine adequate treatment, especially in immunocompromised patients.

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Listeria

McLauchlin¹,

Rees²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

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Lis.te'ri.a. N.L. fem. n. Listeria named after Lord Lister, English surgeon and pioneer of antisepsis. Firmicutes / “Bacilli” / Bacillales / “Listeriaceae” / Listeria Regular, short rods , 0.4–0.5 × 1–2 µm with parallel sides and blunt ends. Usually occur singly or in short chains. In older or rough cultures, filaments of ≥6 µm in length may develop. Gram‐positive with even staining, but some cells, especially in older cultures, lose their ability to retain the Gram strain. Not acid‐fast. Capsules not formed . Do not form spores. All species motile with peritrichous flagella when cultured <30°C. Aerobic and facultative anaerobic . Colonies (24–48 h) are 0.5–1.5 mm in diameter, round, translucent, low convex with a smooth surface and entire margin, non‐pigmented with a crystalline central appearance. May be sticky when removed from agar surfaces, usually emulsify easily, and may leave a slight impression on the agar surface after removal. Older cultures (3–7 d) are larger, 3–5 mm in diameter, have a more opaque appearance, sometimes with a sunken center: rough colonial forms may develop. In 0.25% (w/v) agar, 8% (w/v) gelatin and 1.0% (w/v) glucose semi‐solid medium, growth along the stab after 24 h at 37°C is followed by irregular, cloudy extensions into the medium. Growth spreads slowly through the entire medium. An umbrella‐like zone of maximal growth occurs 3–5 mm below the surface. Temperature limits of growth <0 to 45°C; optimal growth at 30–37°C. Do not survive heating at 60°C for 30 min . Growth occurs between pH 6 and pH 9. Growth occurs in nutrient broth supplemented with up to 10% (w/v) NaCl. Catalase‐positive, oxidase‐negative. Cytochromes produced. Homofermentative anaerobic catabolism of glucose results in production of L (+)‐ lactic acid, acetic acid plus other end products. Acid but no gas produced from other sugars. Methyl‐red‐positive, Voges–Proskauer‐positive . Exogenous citrate not utilized. Organic growth factors are required . Indole is not produced. Esculin and sodium hippurate are hydrolyzed. Urea is not hydrolyzed. Gelatin, casein, and milk are not hydrolyzed. DNA G + C content ( mol %): 36–42.5 ( T m ). Type species : Listeria monocytogenes Pirie 1940a.

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Genetic basis of tetracycline resistance in clinical isolates of Listeria monocytogenes

Cited by 98 publications

References 32 publications

Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis

Presence of the Listeria tetracycline resistance gene tet(S) in Enterococcus faecalis

Antimicrobial susceptibilities of Listeria monocytogenes human strains isolated from 1970 to 2008 in Brazil

Listeria

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