Genetic basis of tetracycline resistance in food-borne isolates of Listeria innocua

Facinelli, Bruna; Roberts, Marilyn C.; Giovanetti, Eleonora; Casolari, Chiara; Fabio, U; Varaldo, Pietro E.

doi:10.1128/aem.59.2.614-616.1993

Cited by 48 publications

(33 citation statements)

References 21 publications

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“…No TCs were observed with the recipient LMK16. This finding is in agreement with other studies of the transfer of tetracycline determinants in Listeria spp., in which the presence of subinhibitory concentrations of tetracycline in the mating medium favoured both intra-and intergeneric dissemination of tet genes (Doucet-Populaire et al, 1991;Facinelli et al, 1993). Transfer of resistance from Lactococcus to Listeria might occur in the aquatic environment and in the fish where the species cohabit, and where subinhibitory levels of tetracycline may occur, due to use of medicated feed.…”

Section: Transfer Of the Tet(s) Gene To L Monocytogenesmentioning

confidence: 99%

“…lactis and cremoris (Moreno et al, 1999;Flórez et al, 2008;Lampkowska et al, 2008). Several studies have described the transfer by conjugation of plasmids and transposons carrying tetracycline resistance genes from Enterococcus and Streptococcus to Listeria, and between Listeria species (Vicente et al, 1988;Facinelli et al, 1993;Perreten et al, 1997), but never from Lactococcus to Listeria.…”

Section: Transfer Of the Tet(s) Gene To L Monocytogenesmentioning

confidence: 99%

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Transfer of plasmid-mediated resistance to tetracycline in pathogenic bacteria from fish and aquaculture environments

Guglielmetti

Korhonen

Heikkinen

et al. 2009

FEMS Microbiology Letters

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The transferability of a large plasmid that harbors a tetracycline resistance gene tet(S), to fish and human pathogens was assessed using electrotransformation and conjugation. The plasmid, originally isolated from fish intestinal Lactococcus lactis ssp. lactis KYA-7, has potent antagonistic activity against the selected recipients (Lactococcus garvieae and Listeria monocytogenes), preventing conjugation. Therefore the tetracycline resistance determinant was transferred via electroporation to L. garvieae. A transformant clone was used as the donor in conjugation experiments with three different L. monocytogenes strains. To our knowledge, this is the first study showing the transfer of an antibiotic resistance plasmid from fish-associated lactic bacteria to L. monocytogenes, even if the donor L. garvieae was not the original host of the tetracycline resistance but experimentally created by electroporation. These results demonstrate that the antibiotic resistance genes in the fish intestinal bacteria have the potential to spread both to fish and human pathogens, posing a risk to aquaculture and consumer safety.

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Section: Transfer Of the Tet(s) Gene To L Monocytogenesmentioning

confidence: 99%

Section: Transfer Of the Tet(s) Gene To L Monocytogenesmentioning

confidence: 99%

Transfer of plasmid-mediated resistance to tetracycline in pathogenic bacteria from fish and aquaculture environments

Guglielmetti

Korhonen

Heikkinen

et al. 2009

FEMS Microbiology Letters

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“…The Tn916 family can mobilize plasmids in trans [79][80][81]. Low levels of tetracycline have been shown to increase transfer and the ability to spread antibiotic resistance genes to other isolates, species and genera [79][80][81]87]. With their transfer ability, one might hypothesize that the ribosomal protection genes would be found in virtually all tetracycline-resistant genera examined, however this is not the case (Tables 1 and 2).…”

Section: Distribution and Mobility Of The Tet Determinantsmentioning

confidence: 99%

“…b All carry the conjugative 25.2 MDa Tet M plasmid with one of the two deletions [99]. c Nine of ten isolates carry incomplete nonmobile Tet M, the tenth carried a mobile Tet M determinant [87]. d M. hominis appears to carry a complete Tet M transposon by Southern hybridization [98], but the Tet M determinants have not been moved from M. hominis but this was without pregrowth in tetracycline [127].…”

Section: Gram-negative Bacteriamentioning

confidence: 99%

Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution

Roberts

1996

FEMS Microbiology Reviews

164

237

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Tetracycline-resistant bacteria were first isolated in 1953 from Shigella dysenteriae, a bacterium which causes bacterial dysentery. Since then tetracycline-resistant bacteria have been found in increasing numbers of species and genera. This has resulted in reduced effectiveness of tetracycline therapy over time. Tetracycline resistance is normally due to the acquisition of new genes often associated with either a mobile plasmid or a transposon. These tetracycline resistance determin~mts are distinguishable both genetically and biochemically. Resistance is primarily due to either energy-dependent efflux Of tetracycline or protection of the ribosomes from the action of tetracycline. Gram-negative tetracycline efflux proteins are linked to repressor proteins which in the absence of tetracycline block transcription of the repressor and structural effiux genes. In contrast, expression of the Gram-positive tetracycline efflux genes and some of the ribosomal protection genes appears to be regulated by attenuation of mRNA transcription. Specific tetracycline resistance genes have been identified in 32 Gram-negative and 22 Gram-positive genera, Tetracycline-resistant bacteria are found in pathogens, opportunistic and normal flora species. Tetracycline-resistant bacteria can be isolated from man, animals, food, and the environment. The nonpathogens in each of these ecosystems may play an important role as reservoirs for the antibiotic resistance genes. It is clear that if we are to reverse the trend toward increasingly antibiotic-resistant pathogenic bacteria we will need to change how antibiotics are used in both human and animal health and food production.

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“…isolates in several studies before (Charpentier and Courvalin 1999). Reasons might be the extensive use of this antimicrobial worldwide, mainly in the past (Facinelli et al 1993;Aarestrup 2012), selecting this resistance in enterococci and staphylococci, and spreading it to Listeria spp. by high-frequency transmissibility of tet(M) due to its presence on various Tn916-like conjugative transposons with a broad host range (Roberts and Mullany 2011).…”

mentioning

confidence: 99%

Antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental Listeria spp. isolates including Listeria monocytogenes

et al. 2014

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The aims of this study were to assess antibiotic resistance pheno-and genotypes in foodborne, clinical, and environmental Listeria isolates, as well as to elucidate the horizontal gene transfer potential of detected resistance genes. A small fraction of in total 524 Listeria spp. isolates (3.1%) displayed acquired antibiotic resistance mainly to tetracycline (n = 11), but also to clindamycin (n = 4) and trimethoprim (n = 3), which was genotypically confirmed. In two cases, a tetracycline resistance phenotype was observed together with a trimethoprim resistance phenotype, namely in a clinical L. monocytogenes strain and in a foodborne L. innocua isolate. Depending on the applied guidelines, a differing number of isolates (n = 2 or n = 20) showed values for ampicillin that are on the edge between intermediate susceptibility and resistance. Transferability of the antibiotic resistance genes from the Listeria donors, elucidated in vitro by filter matings, was demonstrated for genes located on transposons of the Tn916 family and for an unknown clindamycin resistance determinant. Transfer rates of up to 10−5 transconjugants per donor were obtained with a L. monocytogenes recipient and up to 10−7 with an Enterococcus faecalis recipient, respectively. Although the prevalence of acquired antibiotic resistance in Listeria isolates from this study was rather low, the transferability of these resistances enables further spread in the future. This endorses the importance of surveillance of L. monocytogenes and other Listeria spp. in terms of antibiotic susceptibility.

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Genetic basis of tetracycline resistance in food-borne isolates of Listeria innocua

Cited by 48 publications

References 21 publications

Transfer of plasmid-mediated resistance to tetracycline in pathogenic bacteria from fish and aquaculture environments

Transfer of plasmid-mediated resistance to tetracycline in pathogenic bacteria from fish and aquaculture environments

Tetracycline resistance determinants: mechanisms of action, regulation of expression, genetic mobility, and distribution

Antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental Listeria spp. isolates including Listeria monocytogenes

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