Genetic behavior of Triticum aestivum–Dasypyrum villosum translocation chromosomes T6V#4S·6DL and T6V#2S·6AL carrying powdery mildew resistance

Liu, Chang; Ye, Xingguo; Wang, Mei-jiao; Li, Shijin; Lin, Zhishan

doi:10.1016/s2095-3119(16)61568-x

Cited by 10 publications

(8 citation statements)

References 21 publications

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“…However, we found that the exchanging frequency between 6V#2S and 6V#4S was very low in this research. Liu, Ye, Wang, Li, and Lin () also observed the low pairing frequency of two translocation chromosomes. It somewhat suggested that the two D. villosum accessions might have been evolved independently, geographically, for a long history.…”

Section: Discussionmentioning

confidence: 91%

Comparative analysis of genetic effects of wheat‐Dasypyrum villosum translocations T6V#2S·6AL and T6V#4S·6DL

et al. 2019

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Wheat-Dasypyrum villosum translocations T6V#2S·6AL and T6V#4S·6DL, carriers of Pm21 and PmV, respectively, confer high resistance to wheat powdery mildew. For better understanding of the difference in genetic effect between them, a RIL population was constructed based on the cross between "Yangmai 18" carrying T6V#2S·6AL and "Yangmai 22" carrying T6V#4S·6DL. Analysis of distribution of the translocations showed that T6V#2S·6AL is much more transmittable than T6V#4S·6DL. By comparing their effects on main agronomic traits, we firstly found that T6V#2S·6AL contributes greatly to top spikelet fecundity, but causes a decrease of 6.7%-10.5% of spike number. No stable effects of T6V#4S·6DL on agronomic traits were found, except for positive effect on plant height. Excitingly, a new recombinant, T6V#4S-6V#2S·6AL carrying PmV, was screened and proved to have a higher transmission rate than the original translocation T6V#4S·6DL, which will greatly promote the utilization of PmV.The above conclusions of this research will provide important guidance for utilization of Pm21 and PmV more effectively, in wheat powdery mildew resistance breeding. K E Y W O R D Sgenetic effects, molecular markers, powdery mildew, recombinants, T6V#2S·6AL, T6V#4S·6DL

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Section: Discussionmentioning

confidence: 91%

Comparative analysis of genetic effects of wheat‐Dasypyrum villosum translocations T6V#2S·6AL and T6V#4S·6DL

et al. 2019

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“…52 . The seedlings were then covered with preservation film to maintain high humidity and placed in a growth chamber with the same photoperiod as described above and a temperature regime of 22 °C in light/20 °C in darkness.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species

She

Wang

et al. 2017

Sci Rep

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Arginase (ARG) contributes to nitrogen remobilization by conversion of arginine to ornithine and urea. However, wheat ARG genes have not yet been identified. Here we isolated and characterized ARG genes from wheat and its progenitor species and found that a single copy was present in wheat progenitors. Three common wheat ARG genes of TaARG-2AS, TaARG-2BS, and TaARG-2DS were experimentally assigned to the short arms of the group 2 chromosomes. We found an in-frame stop codon in TaARG-2AS, but not in the other two genes. The highest expression was detected in stems and sheaths for TaARG-2BS and in leaves for TaARG-2DS. Both genes have similar expression trend in different developmental stages, peaking at booting and grain filling stages. TaARG-2BS transcript was induced by high salinity and drought, whereas TaARG-2DS was induced by drought only, but neither of them were induced by low temperature. In addition, both genes showed analogous expression pattern upon powdery mildew (PM) infection in the resistant line Pm97033, with TaARG-2BS induced greatly at 72 h post PM infection. In contrast, no obvious transcripts were accumulated for TaARG-2DS in the PM susceptible line Wan7107. Monocot ARGs have more conserved mitochondrion-targeting signals and are more evolutionarily conserved than dicot ARGs.

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“…Current research on D. villosum , including marker development, is mostly focused on the accession D. villosum#2 . Although some preliminary investigations on D. villosum#4 have been performed [11, 22, 31, 37, 39, 56], this genetic resource has not yet been fully exploited and utilized. Developing markers specific to the D. villosum#4 genome will accelerate the application of the potential genes in D. villosum#4 and further expand the genetic background of wheat.…”

Section: Discussionmentioning

confidence: 99%

“…Update, more than 300 D. villosum accessions have been collected from different native habitats, and only a few ( D. villosum #1 to D. villosum #5) have been used to develop wheat- D. villosum chromosome addition, substitution, and translocation lines [6–21]. There is some genetic divergence among different D. villosum accessions [24, 31, 39]. In this study, we were only able to develop one marker each for 1V and 7V.…”

Section: Discussionmentioning

confidence: 99%

“…Wide genetic diversity is known to exist among different original accessions such as D. villosum#2 and D. villosum#4 . Previous studies have shown that there were structural divergences between D. villosum#2 and D. villosum#4 based on cytogenetic observations, 6VS-specific molecular markers, and differing reactions to wheat curl mite [24, 31, 39, 40]. The marker MBH1 can tag both Pm21 and PmV , from 6V#2S and 6V#4S, respectively, showing different sized amplification fragments [24].…”

Section: Introductionmentioning

confidence: 99%

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Development of PCR markers specific to Dasypyrum villosum genome based on transcriptome data and their application in breeding Triticum aestivum-D. villosum#4 alien chromosome lines

Wang

et al. 2019

BMC Genomics

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Background Dasypyrum villosum is an important wild species of wheat ( Triticum aestivum L.) and harbors many desirable genes that can be used to improve various traits of wheat. Compared with other D. villosum accessions, D. villosum#4 still remains less studied. In particular, chromosomes of D. villosum#4 except 6V#4 have not been introduced into wheat by addition or substitution and translocation, which is an essential step to identify and apply the alien desired genes. RNA-seq technology can generate large amounts of transcriptome sequences and accelerate the development of chromosome-specific molecular markers and assisted selection of alien chromosome line. Results We obtained the transcriptome of D. villosum#4 via a high-throughput sequencing technique, and then developed 76 markers specific to each chromosome arm of D. villosum#4 based on the bioinformatic analysis of the transcriptome data. The D. villosum#4 sequences containing the specific DNA markers were expected to be involved in different genes, among which most had functions in metabolic processes. Consequently, we mapped these newly developed molecular markers to the homologous chromosome of barley and obtained the chromosome localization of these markers on barley genome. Then we analyzed the collinearity of these markers among D. villosum , wheat, and barley. In succession, we identified six types of T. aestivum - D. villosum#4 alien chromosome lines which had one or more than one D. villosum#4 chromosome in the cross and backcross BC 3 F 5 populations between T. durum – D. villosum#4 amphidiploid TH3 and wheat cv. Wan7107 by employing the selected specific markers, some of which were further confirmed to be translocation or addition lines by genomic in situ hybridization (GISH). Conclusion Seventy-six PCR markers specific to chromosomes of D. villosum#4 based on transcriptome data were developed in the current study and their collinearity among D. villosum , wheat, and barley were carried out. Six types of Triticum aestivum - D. villosum#4 alien chromosome lines were identified by using 12 developed markers and some of which were further confirmed by GISH. These novel T. aestivum - D. villosum#4 chromosome lines have great potential to be used for the introduction of desirable genes from D. villosum#4 into wheat by chromosomal translocation to breed new wheat varieties. Electronic su...

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Genetic behavior of Triticum aestivum–Dasypyrum villosum translocation chromosomes T6V#4S·6DL and T6V#2S·6AL carrying powdery mildew resistance

Cited by 10 publications

References 21 publications

Comparative analysis of genetic effects of wheat‐Dasypyrum villosum translocations T6V#2S·6AL and T6V#4S·6DL

Comparative analysis of genetic effects of wheat‐Dasypyrum villosum translocations T6V#2S·6AL and T6V#4S·6DL

Comprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species

Development of PCR markers specific to Dasypyrum villosum genome based on transcriptome data and their application in breeding Triticum aestivum-D. villosum#4 alien chromosome lines

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