Genetic characterisation of Cryptosporidium from a wild population of eastern grey kangaroos Macropus giganteus inhabiting a water catchment

Power, Michelle; Slade, Martin B.; Sangster, Nicholas C.; Veal, Duncan

doi:10.1016/j.meegid.2004.01.002

Cited by 51 publications

(43 citation statements)

References 24 publications

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“…Variation of any given haplotype from the consensus was detected at 2 to 5 positions (0.35 to 0.87%). Similar vari-ability has been reported at this locus for other Cryptosporidium genotypes (14), and it is probably explained by the particular organization of this gene in cryptosporidia (11). The actin product aligned well with the reference C. parvum sequence, matching positions 19 to 1,122 of its coding region (amino acids 7 to 373 of the translated peptide).…”

supporting

confidence: 82%

Molecular Characterization of Cryptosporidium molnari Reveals a Distinct Piscine Clade

Palenzuela

Álvarez-Pellitero

Sitjà‐Bobadilla

2010

Appl Environ Microbiol

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Multilocus phylogenetic analysis of small-subunit (SSU) rRNA and actin from Cryptosporidium molnari clustered this species with the C. molnari-like genotype of an isolate from the guppy, although the two fish isolates seem to be distinct species. The analysis of available piscine genotypes provides some support for cladistic congruence of the genus Piscicryptosporidium, but additional piscine genotypes are needed.

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supporting

confidence: 82%

Molecular Characterization of Cryptosporidium molnari Reveals a Distinct Piscine Clade

Palenzuela

Álvarez-Pellitero

Sitjà‐Bobadilla

2010

Appl Environ Microbiol

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“…Previous multilocus analyses of six Cryptosporidium isolates arising from this study identified three isolates representative of two Cryptosporidium "marsupial" genotypes: Cryptosporidium marsupial genotype I isolates EGK1 and EGK2 and the Cryptosporidium marsupial genotype II (21). The frequencies of these isolates in the kangaroo population were determined by partial 18S rRNA gene sequencing of 51 isolates representing 10 of the 11 fecal sampling periods.…”

Section: Methodsmentioning

confidence: 88%

“…Observation of Cryptosporidium in Australian marsupials is limited to information gained from animals in captivity or undergoing rehabilitation. Characterization of Cryptosporidium oocysts collected from marsupial species have identified two marsupial-derived genotypes that have a broad marsupial host range (15,21,34). The Cryptosporidium "marsupial" genotype I has been identified in captive koalas (Phascolarctos cinereus) (isolates K1 and K3) (16), a juvenile red kangaroo (Macropus rufus) (isolate K2) (34), a captive yellow-footed rock wallaby (Petrogale xanthopus), and eastern grey kangaroos (Macropus giganteus) (isolate EGK2) (21).…”

mentioning

confidence: 99%

“…Characterization of Cryptosporidium oocysts collected from marsupial species have identified two marsupial-derived genotypes that have a broad marsupial host range (15,21,34). The Cryptosporidium "marsupial" genotype I has been identified in captive koalas (Phascolarctos cinereus) (isolates K1 and K3) (16), a juvenile red kangaroo (Macropus rufus) (isolate K2) (34), a captive yellow-footed rock wallaby (Petrogale xanthopus), and eastern grey kangaroos (Macropus giganteus) (isolate EGK2) (21). Two further Cryptosporidium genotypes have been identified in wild eastern grey kangaroos, a variant of the Cryptosporidium "marsupial" genotype I (isolate EGK1) and the Cryptosporidium marsupial genotype II (21).…”

mentioning

confidence: 99%

“…The Cryptosporidium "marsupial" genotype I has been identified in captive koalas (Phascolarctos cinereus) (isolates K1 and K3) (16), a juvenile red kangaroo (Macropus rufus) (isolate K2) (34), a captive yellow-footed rock wallaby (Petrogale xanthopus), and eastern grey kangaroos (Macropus giganteus) (isolate EGK2) (21). Two further Cryptosporidium genotypes have been identified in wild eastern grey kangaroos, a variant of the Cryptosporidium "marsupial" genotype I (isolate EGK1) and the Cryptosporidium marsupial genotype II (21). Understanding patterns and degrees of Cryptosporidium oocyst shedding by native animals, their potential contribution to water contamination, and the significance to human health is important for formulating catchment management strategies, including risk assessment and interpretation of results from raw water analyses.…”

mentioning

confidence: 99%

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Patterns ofCryptosporidiumOocyst Shedding by Eastern Grey Kangaroos Inhabiting an Australian Watershed

Power

Sangster

Slade

et al. 2005

Appl Environ Microbiol

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The occurrence of Cryptosporidium oocysts in feces from a population of wild eastern grey kangaroos inhabiting a protected watershed in Sydney, Australia, was investigated. Over a 2-year period, Cryptosporidium oocysts were detected in 239 of the 3,557 (6.7%) eastern grey kangaroo fecal samples tested by using a combined immunomagnetic separation and flow cytometric technique. The prevalence of Cryptosporidium in this host population was estimated to range from 0.32% to 28.5%, with peaks occurring during the autumn months. Oocyst shedding intensity ranged from below 20 oocysts/g feces to 2.0 ؋ 10 6 oocysts/g feces, and shedding did not appear to be associated with diarrhea. Although morphologically similar to the human-infective Cryptosporidium hominis and the Cryptosporidium parvum "bovine" genotype oocysts, the oocysts isolated from kangaroo feces were identified as the Cryptosporidium "marsupial" genotype I or "marsupial" genotype II. Kangaroos are the predominant large mammal inhabiting Australian watersheds and are potentially a significant source of Cryptosporidium contamination of drinking water reservoirs. However, this host population was predominantly shedding the marsupial-derived genotypes, which to date have been identified only in marsupial host species.

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Specific and genotypic identification of Cryptosporidium from a broad range of host species by nonisotopic SSCP analysis of nuclear ribosomal DNA

Jex

Ryan

et al. 2007

Electrophoresis

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The accurate identification of Cryptosporidium (Protozoa: Apicomplexa) species and genotypes is central to the understanding of the transmission and to the diagnosis and control of cryptosporidiosis. In this study, we demonstrate the effectiveness of nonisotopic SSCP analysis of a approximately 300 bp region of the small subunit (pSSU) of ribosomal DNA for the specific identification of and delineation among 18 different Cryptosporidium species and genotypes from a wide range of hosts. This mutation scanning approach allowed the rapid and reliable differentiation between species/genotypes differing by as little as 1.3% in the pSSU sequence, with the capacity to detect point mutations. The present findings confirm the usefulness of this tool for the rapid genetic screening of Cryptosporidium samples from any host species, providing a foundation for detailed systematic, epidemiological and ecological studies. Although applied herein to pSSU, this low cost approach should be applicable to a wide range of genetic loci for population genetic investigations of Cryptosporidium.

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Genetic characterisation of Cryptosporidium from a wild population of eastern grey kangaroos Macropus giganteus inhabiting a water catchment

Cited by 51 publications

References 24 publications

Molecular Characterization of Cryptosporidium molnari Reveals a Distinct Piscine Clade

Molecular Characterization of Cryptosporidium molnari Reveals a Distinct Piscine Clade

Patterns ofCryptosporidiumOocyst Shedding by Eastern Grey Kangaroos Inhabiting an Australian Watershed

Specific and genotypic identification of Cryptosporidium from a broad range of host species by nonisotopic SSCP analysis of nuclear ribosomal DNA

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