Genetic Characteristics and Microbiological Profile of Hypermucoviscous Multidrug-Resistant Klebsiella variicola Coproducing IMP-4 and NDM-1 Carbapenemases

Wang, Bingjie; Pan, Fang; Han, Dingding; Zhao, Wantong; Shi, Yingying; Sun, Yi-Ming; Wang, Chun; Zhang, Tiandong; Zhang, Hong

doi:10.1128/spectrum.01581-21

Cited by 6 publications

(6 citation statements)

References 19 publications

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“…Among these, p4253-imp exhibited higher similarity to pNDM-IMP-1, with 99% identity and 100% query coverage. pNDM-IMP-1 is a plasmid carrying bla IMP−4 from a urine catheter isolate of K. variicola SHET-01, obtained from a 2-year-old girl in the pediatric intensive care unit of a teaching hospital in Shanghai, China [ 11 ]. Gene comparison analysis (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…It is possible that the qacG gene cassette was lost during the dissemination process. In809 was first described in Acinetobacter species isolated from Hong Kong between 1997 and 2000 [ 29 ] and has been subsequently reported in various Acinetobacter [ 30 ]and Enterobacteriaceae isolates [ 11 , 25 , 31 ]. This suggests that the bla IMP−4 array may be mobilized in Acinetobacter and Enterobacteriaceae through homologous recombination or plasmid-mediated horizontal transfer.…”

Section: Discussionmentioning

confidence: 99%

“…It is a gram-negative, facultative anaerobic, non-spore-forming, and non-motile rod-shaped bacterium that forms circular, convex, and smooth colonies [ 7 ]. In recent years, with the updating of the MALDI-TOF database and the widespread use of genomic sequencing, there has been an increasing number of reports on isolates of K. variicola , including the emergence of highly virulent K. variicola strains resistant to colistin [ 8 ], the presence of a hypervirulent conjugative plasmid in K. variicola [ 9 ], and the coexistence of bla NDM−1 and bla IMP−4 genes on one plasmid in K. variicola [ 10 , 11 ]. Compared to K. pneumoniae , typically, K. variicola isolates exhibit lower antibiotic resistance rates [ 12 ], but studies have shown a high mortality rate associated with bloodstream infections caused by multidrug-resistant and extended-spectrum β-lactamase-producing K. variicola [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

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Coexistence of blaIMP−4 and blaSFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain

Chen,

Xu,

Liu

et al. 2024

Ann Clin Microbiol Antimicrob

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Background Klebsiella variicola is considered a newly emerging human pathogen. Clinical isolates of carbapenemase and broad-spectrum β-lactamase-producing K. variicola remain relatively uncommon. A strain of K. variicola 4253 was isolated from a clinical sample, and was identified to carry the blaIMP−4 and blaSFO−1 genes. This study aims to discern its antibiotic resistance phenotype and genomic characteristics. Methods Species identification was conducted using MALDI-TOF/MS. PCR identification confirmed the presence of the blaIMP−4 and blaSFO−1 genes. Antibiotic resistance phenotype and genomic characteristics were detected by antimicrobial susceptibility testing and whole-genome sequencing. Plasmid characterization was carried out through S1-PFGE, conjugation experiments, Southern blot, and comparative genomic analysis. Results K. variicola 4253 belonged to ST347, and demonstrated resistance to broad-spectrum β-lactamase drugs and tigecycline while being insensitive to imipenem and meropenem. The blaIMP−4 and blaSFO−1 genes harbored on the plasmid p4253-imp. The replicon type of p4253-imp was identified as IncHI5B, representing a multidrug-resistant plasmid capable of horizontal transfer and mediating the dissemination of drug resistance. The blaIMP−4 gene was located on the In809-like integrative element (Intl1-blaIMP−4-aacA4-catB3), which circulates in Acinetobacter and Enterobacteriaceae. Conclusions This study reports the presence of a strain of K. variicola, which is insensitive to tigecycline, carrying a plasmid harboring blaIMP−4 and blaSFO−1. It is highly likely that the strain acquired this plasmid through horizontal transfer. The blaIMP−4 array (Intl1-blaIMP−4-aacA4-catB3) is also mobile in Acinetobacter and Enterobacteriaceae. So it is essential to enhance clinical awareness and conduct epidemiological surveillance on multidrug-resistant K. variicola, conjugative plasmids carrying blaIMP−4, and the In809 integrative element.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Coexistence of blaIMP−4 and blaSFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain

Chen,

Xu,

Liu

et al. 2024

Ann Clin Microbiol Antimicrob

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“…The ability of selected K. pneumoniae strains to form biofilms was determined as described previously ( 54 ). An overnight bacterial culture was suspended with LB broth at 0.5 McFarland, then was diluted 1:100 and transferred to 96-well plates with 200 μL LB medium.…”

Section: Methodsmentioning

confidence: 99%

Association between Intestinal Colonization and Extraintestinal Infection with Carbapenem-Resistant Klebsiella pneumoniae in Children

Pan

et al. 2023

Microbiol Spectr

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Carbapenem-resistant Klebsiella pneumoniae (CRKP) causes an increasing number of nosocomial infections, which can be life-threatening, as carbapenems are last-resort antibiotics. K. pneumoniae is part of the healthy human microbiome, and this provides a potential advantage for infection. This study demonstrated that CRKP intestinal colonization is strongly linked to extraintestinal infection, based on the evidence given by whole-genome sequencing data and phenotypic assays of antimicrobial resistance and virulence.

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“…4 A hypermucoviscous multidrug-resistant K. variicola coproducing IMP-4 and NDM-1 was obtained from a pediatric patient. 5 Multidrug-resistant K. variicola has caused outbreaks in a Bangladeshi neonatal unit with high mortality. Given all that, more attention should be paid to K. variicola, which may be a reservoir of antimicrobial resistance genes.…”

Section: Introductionmentioning

confidence: 99%

Emergence of OXA-484-Producing Klebsiella variicola in China

Ge¹,

Qiao²,

Xu³

et al. 2023

IDR

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The frequent and inappropriate use of antibiotics has caused a dramatic rise in the number, species, and degree of multi-drug resistant bacteria, making them more prevalent and difficult to treat. In this context, the aim of the present study was to characterize the OXA-484-producing strains isolated from a perianal swab of a patient by using whole-genome analysis. Patients and Methods: In this study, carbapenemase-producing Klebsiella variicola was identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), average nucleotide identity (ANI) and PCR. S1 nuclease pulsed-field gel electrophoresis (S1-PFGE) and Southern blotting were utilized to characterize the plasmid profiles of K. variicola 4717. In particular, WGS was performed to obtain genomic information on this clinical isolate, and assemble all the plasmids of the bla OXA-484 -harboring strain. Results: The antimicrobial susceptibility pattern of K. variicola 4717 revealed that it was resistant to a range of antibiotics, including aztreonam, imipenem, meropenem, ceftriaxone, cefotaxime, ceftazidime, levofloxacin, ciprofloxacin, piperacillin-tazobactam, methylenesulfamer oxazole, amoxicillin-clavulanic acid, cefepime, and tigecycline. Its susceptibility to chloromycin was intermediate, while it was still susceptible to amikacin, gentamicin, fosfomycin, and polymyxin B. The presence of two companion plasmids, p4717_1 and p4717_2, together with a plasmid carrying the bla OXA-484 gene was observed. An in-depth investigation of p4717-OXA-484 uncovered that it is an IncX3-type plasmid and shares a similar segment encoded by IS26. Given the similar genetic background, it was conceivable that bla OXA-484 could have developed from bla OXA-181 through a series of mutations. Conclusion: Herein, we described the first genome sequence of K. variicola strain harbouring the class D β-actamase bla OXA-484 in an Inc-X3-type plasmid. Our work also uncovered the genetic characterization of K. variicola 4717 and the importance of initiating antimicrobial detection promptly.

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Genetic Characteristics and Microbiological Profile of Hypermucoviscous Multidrug-Resistant Klebsiella variicola Coproducing IMP-4 and NDM-1 Carbapenemases

Abstract: As an important member of the Klebsiella pneumoniae complex, Klebsiella variicola is poorly studied as an emerging human pathogen. We, for the first time, report a unique K. variicola isolated from a pediatric patient in China.

Cited by 6 publications

References 19 publications

Coexistence of blaIMP−4 and blaSFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain

Coexistence of blaIMP−4 and blaSFO−1 in an IncHI5B plasmid harbored by tigecycline-non-susceptible Klebsiella variicola strain

Association between Intestinal Colonization and Extraintestinal Infection with Carbapenem-Resistant Klebsiella pneumoniae in Children

Emergence of OXA-484-Producing Klebsiella variicola in China

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