Genetic characterization and biofilm formation of potentially pathogenic foodborne Arcobacter isolates

Martínez-Malaxetxebarria, Irati; Girbau, Cecilia; Salazar-Sánchez, Adrián; Baztarrika, Itsaso; Martínez-Ballesteros, Ilargi; Laorden, Lorena; Alonso, Rodrigo; Fernández‐Astorga, Aurora

doi:10.1016/j.ijfoodmicro.2022.109712

Cited by 12 publications

(12 citation statements)

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“…Four A. butzleri strains were selected for mutagenesis assays of biofilm-associated genes based on their different ability to form biofilms [ 5 , 14 ]. All of them had been previously isolated from different food products at retail [ 2 , 5 ] and presented biofilm-associated genes, as confirmed by PCR (see the following section). The reference strain A. butzleri RM 4018 was also included.…”

Section: Methodsmentioning

confidence: 99%

“…Arcobacter butzleri is a Gram-negative bacterium with a wide environmental distribution, classified as a foodborne pathogen [ 1 ] due to its association with human gastrointestinal disease. A. butzleri is the most prevalent among the species of the genus and is frequently isolated from wild and farm animals’ excrements and intestinal regions (boar, ostrich, Eurasian collared dove and raccoon), farm animals’ meat (chicken, pork, beef, turkey, lamb, sheep, rabbit and quail meat), seafood products (clam, mussel, cockle, squid and shrimp), dairy products (raw cow milk and fresh cheese), vegetables (carrot, spinach, lettuce, chard, parsley, arugula and radish), environmental water and human stool [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

“…Many food-related pathogens such as Aeromonas spp., Salmonella Typhimurium, Staphylococcus aureus , Listeria monocytogenes , L. ivanovii , Escherichia coli , Bacillus cereus , Cronobacter sakazakii , C. muytjensii , A. butzleri , A. cryaerophilus , Pseudomonas aeruginosa , Klebsiella pneumoniae , Campylobacter jejuni and C. coli can produce these resistance structures conformed by cells and extracellular compounds [ 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ]. The biofilm formation ability of Arcobacter species on different surfaces has also been documented [ 5 , 25 ], and it has become apparent that there is a notorious variability of the adherence level among strains of the same species. Due to the difficulty of eradicating biofilms and the rapidity with which A. butzleri develops them, preventing their formation is vital to control the spread of this foodborne pathogen [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

“…A. butzleri presents homologous of the genes flaA , flaB , fliS , luxS , pta and spoT [ 5 ], but their function related to the biofilm formation has not been established yet. Nevertheless, it is reasonable to think that they may also affect adherence and biofilm formation in A. butzleri .…”

Section: Introductionmentioning

confidence: 99%

“…2 NCCB: Netherlands Culture Collection of Bacteria 3. Data obtained from Martinez-Malaxetxebarria et al[5] and Girbau et al[14]. Values are expressed as mean ± standard errors.…”

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confidence: 99%

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Arcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formation

et al. 2022

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Arcobacter butzleri, the most prevalent species of the genus, has the demonstrated ability to adhere to various surfaces through biofilm production. The biofilm formation capability has been related to the expression of certain genes, which have not been characterized in A. butzleri. In order to increase the knowledge of this foodborne pathogen, the aim of this study was to assess the role of six biofilm-associated genes in campylobacteria (flaA, flaB, fliS, luxS, pta and spoT) in the biofilm formation ability of A. butzleri. Knockout mutants were constructed from different foodborne isolates, and static biofilm assays were conducted on polystyrene (PS), reinforced glass and stainless steel. Additionally, motility and Congo red binding assays were performed. In general, mutants in flaAB, fliS and luxS showed a decrease in the biofilm production irrespective of the surface; mutants in spoT showed an increase on stainless steel, and mutants in pta and spoT showed a decrease on reinforced glass but an increase on PS. Our work sheds light on the biofilm-related pathogenesis of A. butzleri, although future studies are necessary to achieve a satisfactory objective.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…2 NCCB: Netherlands Culture Collection of Bacteria 3. Data obtained from Martinez-Malaxetxebarria et al[5] and Girbau et al[14]. Values are expressed as mean ± standard errors.…”

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Arcobacter butzleri Biofilms: Insights into the Genes Beneath Their Formation

et al. 2022

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Investigation and characterization of Aliarcobacter spp. isolated from cattle slaughterhouse in Türkiye

Disli,

Hizlisoy,

Gungor

et al. 2024

Int Microbiol

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Aliarcobacter spp. have has been isolated from numerous food products at retail and from animal carcasses and feces at slaughter. The objectives of this study were: i) to isolate Aliarcobacter species (Aliarcobacter butzleri, Aliarcobacter skirrowii, Aliarcobacter cryaerophilus) from different slaughterhouses' samples, ii) to detect genetic diversity, antibiotic resistance, bio lm ability and putative virulence gene pro les of the isolates. The antibiotic susceptibility to antimicrobials was examined using the Kirby-Bauer disc diffusion method. A molecular investigation of antibiotic resistance and virulence factors was also conducted using polymerase chain reaction (PCR).A total of 22 (14.6%) Aliarcobacter spp. isolates were obtained, with varying levels of antibiotic resistance observed. The genes tetO, tetW, and gyrA were detected in 0%, 31.8%, and 27.2% of the isolates, respectively. All isolates were resistant to ampicillin, rifampin, and erythromycin, while tetracycline was found to be the most effective antibiotic, with 81.8% of the isolates showing susceptibility to it. In terms of virulence factors, all isolates (100%) harbored more than one of the nine putative virulence genes tested, with 18.1% of isolates carrying more than three.Regarding bio lm formation, 7 (31.8%) and 4 (18.1%) isolates were found to form strong and moderate bio lms, respectively, while one (4.5%) isolate was classi ed as a weak bio lm producer. ERIC-PCR band patterns suggested that the isolated Aliarcobacter spp. from slaughterhouses had different sources of contamination. These ndings highlight the potential risk posed by pathogenic and multidrug-resistant Aliarcobacter spp. in food, and the need for control measures throughout the food chain to prevent the spread of these strains. The results indicate that foods of animal origin and cattle slaughterhouses are signi cant sources of antimicrobial resistant Aliarcobacters.

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