Genetic characterization and species relationships among selected Asiatic Vigna Savi

Abstract

Twenty-four ISSR primers were employed for diversity analysis, genotyping and assessment of species relationships in Asiatic Vigna from the Indian subcontinent. A total of 396 fragments were scored with an average of 16.5 products per primer. Primer no 827 was the most informative primer with highest resolving power (Rp), marker index (MI) as well as genotypic index (GI). Linear correlation was observed between Rp, MI and GI. The wild forms, V. mungo var. silvestris, V. radiata var. sublobata and V. radiata var. setulosa grouped closer to their respective cultigens. V. hainiana emerged as a distinct taxon and seems to be more primitive in comparison to the other close wild relatives of greengram and blackgram. Moderate gene flow and genetic differentiation was detected between the species analyzed. Analysis of molecular variance performed for the group (mungo-radiata-hainiana) in three different combinations revealed that nearly half of the variation exists within the populations while the remaining half is equally distributed among species and among populations within species.

Pollen morphology of Asian Vigna species (genus Vigna; subgenus Ceratotropis)from India and its taxonomic implications

“…The sectional classification of subgenus Ceratotropis was confirmed by several researchers (Jaaska and Jaaska, 1990;Kaga et al, 1996;Doi et al, 2002;Konarev et al, 2002;Tomooka et al, 2002aTomooka et al, , 2002bSaini et al, 2008). Moreover, morphological studies and molecular approaches have been used efficiently for the taxonomy of the subgenus Ceratotropis (Verdcourt, 1970;Maréchal et al, 1978;Egawa and Tomooka, 1994;Lawn, 1995;Tateishi, 1996;Doi et al, 2002;Goel et al, 2002;Tateishi and Maxted, 2002;Tomooka et al, 2002b;Bisht et al, 2005;Tun and Yamaguchi, 2007;Saini et al, 2008;Saini and Jawali, 2009;Vir et al, 2010;Dixit et al, 2011;Javadi et al, 2011;Aitawade et al, 2012;Latha et al, 2014;Yadav et al, 2014).…”

Rice bean: a lesser known pulse with well-recognized potential

“…A number of workers attempted to elucidate phylogenetic relationship within the subgenera Ceratotropis using isozymes (Jaaska andJaaska 1990, Vaillancourt andWeeden 1993), RAPD (Fatokun et al 1993a), RFLP (Fatokun et al 1993b), AFLP (Tomooka et al 2002b;Seehalak et al 2006), etc. In addition, antigenic peptide (Kajiwara and Tomooka 1998), rDNA ITS and atpB-rbcl intergenic spacer (Doi et al 2002), ISSR (Vir et al 2010) and plastid DNA sequences (Javadi et al 2011) were used to decipher phylogenetic relationship in the subgenus. Genetic relatedness of 44 Vigna accessions belonging to four subgenera was assessed using RFLP analysis (Fatokun et al 1993b).…”

“…The distinctness of the Asiatic grams in subgenus Ceratotropis was elucidated by this study. Vir et al (2010) (Doi et al 2002). These findings were supported by a recent study using seventy-one Vigna accessions, consisting of 28 species and three subgenera (Ceratotropis, Plectrotropis, and Vigna) conserved at the National Institute of Agrobiological Sciences, Japan (Takahashi et al 2016).…”

Application of ISSR markers for verification of F1 hybrids in mungbean (Vigna radiata)

“…Because of its simplicity, efficiency, high reproducibility, and non-reliance on prior genomic sequence information, it has been widely used in applications including genetic diversity, genetic relationship, and germplasm analyses, seed purity evaluations, gene/quantitative trait loci mapping, marker-assisted selection, and evolutionary study in various plants including mungbean (Ajibade et al, 2000;Reddy et al, 2002;Wong et al, 2005;Somta and Srinives, 2007;Abbas et al, 2010;Tantasawat et al, 2010a,b;Vir et al, 2010;Li et al, 2011;Mudibu et al, 2011). Its application in the identification and confirmation of hybrids and parentage has also been reported in artichoke, bamboo, clover, mandarin, Penstemon, and the Triticeae tribe (Wolfe et al, 1998;Scarano et al, 2002;Carvalho et al, 2005;Dabkevičienė et al, 2008;Lin et al, 2010;Bianco et al, 2011).…”