Genetic characterization of canine parvovirus type 2 subtypes in Maputo, Mozambique

Figueiredo, J.; Miranda, Carla; Souto, Ricardo Peres do; Silva, E.; Fafetine, José; Thompson, Gertrude

doi:10.1007/s00203-016-1320-7

Cited by 14 publications

(13 citation statements)

References 40 publications

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“…During years, CPV spread and evolution have been well documented in North and South America, Europe and Asia (Miranda & Thompson, ; Zhou, Zeng, Zhang, & Li, ). More recently, data about its spread were also obtained from Australia and Africa (Amrani et al, ; Castanheira et al, ; Chollom et al, ; Dogonyaro et al, ; Figuiredo et al, ; Folitse et al, ; Kapiya et al, ; Touhiri et al, ; Woolford et al, ). Most of these studies were based on the partial or complete VP2 gene sequence, due to the involvement of the VP2 capsid protein in host switch and to its fast evolutionary rate (Hueffer et al, ; Nelson, Palermo, Hafenstein, & Parrish, ; Shackelton, Parrish, Truyen, & Holmes, ), with limited information on other CPV encoding gene sequences.…”

Section: Discussionmentioning

confidence: 99%

“…In the African continent, CPV has been described in South Africa and Namibia (Dogonyaro, Bosman, Sibeko, Venter, & Vuuren, ; Steinel, Venter, Van Vuuren, Parrish, & Truyen, ), Zambia (Kapiya et al, ), Mozambique (Figuiredo et al, ), Ghana (Folitse et al, ), Morocco (Amrani et al, ), Cape Verde (Costanheira et al, ), Nigeria (Chollom et al, ) and Tunisia (Touhiri et al, ). In Nigeria, only recently the molecular analyses based on the partial VP2 gene sequence of CPV strains described the circulating CPV variants (Apaa, Daly, & Tarlinton, ; Dogonyaro et al, ; Fagbohun & Omobowale, ).…”

Section: Introductionmentioning

confidence: 99%

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Nearly full‐length genome characterization of canine parvovirus strains circulating in Nigeria

Ogbu

Mira

Purpari

et al. 2019

Transbound Emerg Dis

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Canine parvovirus type 2 (CPV‐2) emerged suddenly in the late 1970s as pathogen of dogs, causing a severe and often fatal gastroenteric disease. The original CPV‐2 was replaced by three antigenic variants, CPV‐2a, CPV‐2b and CPV‐2c, which to date have gained a worldwide distribution with different relative proportions. All previous studies conducted in Africa were based on partial VP2 gene sequences. The aim of this study was to provide a genome analysis to characterize the CPV strains collected in Nigeria, Africa. Rectal swab samples (n = 320) were collected in 2018 and tested by means of an immunochromatographic assay. Among the 144 positive samples, 59 were selected for further analyses using different molecular assays. The results revealed a high prevalence of CPV‐2c (91.5%) compared to the CPV‐2a variant (8.5%). The VP2 gene sequences showed a divergence from the strains analysed in 2010 in Nigeria and a closer connection with CPV strains of Asian origin. The non‐structural gene analysis evidenced amino acid changes never previously reported. The molecular analysis based on genomic sequences evidenced a geographical pattern of distribution of the analysed strains, suggesting a potential common evolutionary origin with CPV of Asian origin. This study represents the first CPV molecular characterization including all the encoding gene sequences conducted in the African continent and contributes to define the current geographical spread of the CPV variants worldwide.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nearly full‐length genome characterization of canine parvovirus strains circulating in Nigeria

Ogbu

Mira

Purpari

et al. 2019

Transbound Emerg Dis

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“…According to Demeter et al (2010), Mbo II-based RFLP analysis is misleading for detection of CPV-2c because CPV-2a can show the same RFLP results as CPV 2c. However, Figueiredo et al (2017) showed that CPV-positive PCR amplicons were not digested with Mbo II enzyme and identified as CPV-2/2a/2b. This suggests that Mbo II-based RFLP analysis is unreliable if used alone to identify CPV-2 variants in samples.…”

Section: Discussionmentioning

confidence: 99%

Molecular and restriction fragment length polymorphism analysis of canine parvovirus 2 (CPV-2) in dogs in southeast Anatolia, Turkey

Polat

Şahan

Aksoy

et al. 2019

Onderstepoort Journal of Veterinary Research

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Canine parvovirus-2 (CPV-2) is the aetiological agent of an infectious viral disease of dogs, characterised by diarrhoea and vomiting. Mutations of the CPV-2 genome have generated new variants circulating worldwide. This article reports the molecular analysis of CPV-2 variants collected in the dog population in southeast Anatolia, Turkey. Twenty blood samples previously taken for the laboratory diagnosis of dogs with suspected parvovirus were screened for CPV-2 by polymerase chain reaction (PCR). Of the 20 samples, 18 tested positive for CPV-2. Partial VP2 gene sequencing and restriction fragment length polymorphism (RFLP) analysis revealed CPV-2a (n = 1), CPV-2b (n = 16) and CPV-2c (n = 1) variants. Phylogenetic analysis based on the partial length VP2 gene showed that CPV-2b (n = 15) variants showed sequences clustering separately in the phylogenetic tree. The CPV-2c sample was phylogenetically related to Chinese strains and Indonesia strain, whereas the CPV-2a sample was phylogenetically related to the Portuguese strain. These results, which are the first to demonstrate the presence of CPV-2c in the dog population of southeast Anatolia, Turkey, indicate that CPV-2a/2b/2c variants co-exist in Turkey’s dog population.

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“…In Australia, New Zealand, and Oceania, investigation of CPV in dogs has demonstrated that CPV-2a remains the predominant genetic variant, and has not been replaced by CPV-2b or CPV-2c, as in many other countries [83,84] . In Africa, infection of dogs with either CPV-2a or -2b was VANNAMAHAXAY, CHUAMMITRI reported in Nigeria, Mozambique, and South Africa [85,86] ; however, the CPV-2c strain was found to be present in Morocco [87] . The numbers and percentages of detected CPV cases are summarized by region in Fig.…”

Section: Prevalence Of Canine Parvovirus Type 2 Variantsmentioning

confidence: 95%

Canine Parvoviruslar Üzerine Güncelleme: Canine Konakçıyı Etkileyen Genetik Varyasyonlara Vurgu Yaparak Moleküler ve Genomik Yönler

Vannamahaxay¹,

Chuammitri²

2017

Kafkas Univ Vet Fak Derg

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Citation of This ArticleVannamahaxay S, Chuammitri P: Update on canine Parvovirus: Molecular and genomic aspects, with emphasis on genetic variants affecting the canine host. Kafkas Univ Vet Fak Derg, 23 (5): 847-856, 2017847-856, . DOI: 10.9775/kvfd.2017 AbstractCanine parvovirus (CPV), the etiology of hemorrhagic enteritis in dogs, was first isolated as CPV type 2 (CPV-2) almost 40 years ago, and was soon replaced by the emergence of new variant types. The major viral capsid proteins encoded by the VP2 gene are the sites where amino acids are often substituted, accounting for the unusual nature of this type of DNA virus. The alteration of specific residues has contributed to different antigenic variants which have affected the evolution of virus binding and host immunity to this virus. Sequence analysis of the VP2 gene and subsequent characterization have revealed three circulating CPV-2 strains, CPV-2a, CPV-2b, and CPV-2c, identified by mutations at amino acid residue 426. The latter strain displays increased pathogenicity in dogs and an extended host range. The present review article aimed at updating contemporary information on epidemiological studies and surveys from CPV field work. Moreover, we pointed out some sensitive and rapid diagnostic tools for detecting CPV in clinical samples, techniques which will be useful for health monitoring and management of CPV with currently available vaccines. Keywords: Canine Parvovirus, CPV type 2, Genetic Variation, VP2 Gene, Mutation, Dog Köpek Parvovirusu Üzerine Bir Güncelleme: Köpek Konakçıya Etki Eden Genetik Varyasyonların Moleküler ve Genomik Özellikleri ÖzetKöpeklerde hemorajik enteritin etiyolojik etkeni olan Canine parvovirus (CPV) neredeyse 40 yıl önce ilk olarak CPV tip 2 (CPV-2) olarak izole edildi ve hemen sonrasında ortaya çıkan yeni varyant tipler CPV tip 2'nin yerine geçti. VP2 geni tarafından kodlanan major viral kapsid proteinler aynı zamanda en sıklıkla amino asitlerin başka amino asitlerle değiştiği alanlar olup bu tip DNA virusların aykırı doğasının da sebebini oluşturmaktadır. Spesifik yapılardaki değişimler farklı antijenik varyantların oluşmasına katkıda bulunarak bu virüsün bağlanma ve virusa karşı konakçı bağışıklığının değişmesini etkilemiştir. VP2 geninin sekans analizi ve takibinde karakterizasyonu, 426. amino asitte mutasyon ile şekillenen CPV2a, CPV-2b ve CPV-2c olmak üzere dolaşımda 3 farklı CPV-2 suşunun olduğunu göstermiştir. CPV-2c köpeklerde artmış patojenite ve daha geniş konakçı yelpazesi göstermektedir. Bu derlemede güncel epidemiyolojik çalışmalar ile CPV saha çalışmaları hakkındaki bilgilerin güncellenmesi amaçlanmıştır. Ayrıca, klinik örneklerde CPV'nin tanısında kullanılmak suretiyle sağlık taramasında faydalı olabilecek ve mevcut aşılarla CPV'nin kontrol altına alınmasında faydalı olabilecek bazı hassas ve hızlı tanı yöntemleri değerlendirilmiştir.

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Genetic characterization of canine parvovirus type 2 subtypes in Maputo, Mozambique

Cited by 14 publications

References 40 publications

Nearly full‐length genome characterization of canine parvovirus strains circulating in Nigeria

Nearly full‐length genome characterization of canine parvovirus strains circulating in Nigeria

Molecular and restriction fragment length polymorphism analysis of canine parvovirus 2 (CPV-2) in dogs in southeast Anatolia, Turkey

Canine Parvoviruslar Üzerine Güncelleme: Canine Konakçıyı Etkileyen Genetik Varyasyonlara Vurgu Yaparak Moleküler ve Genomik Yönler

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