Genetic characterization of four Algerian goat breeds assessed by microsatellite markers

Hakim, Tefiel; Ata, Nezi̇h; Chahbar, Mohamed; Benyarou, Mohamed; Fantazi, Khaled; Yılmaz, Onur; Cemal, İ̇brahim; Karaca, Orhan; Boudouma, D.; Gaouar, Semir Bechir Suheil

doi:10.1016/j.smallrumres.2018.01.021

Cited by 22 publications

(28 citation statements)

References 42 publications

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“…Although the obtained average PE value (0.635), which is known as a measure of efficiency in paternity testing, was in the probability of exclusion with some literature (20,22), it was higher than values obtained from other studies (19). This may have been due to the differences in microsatellite loci used in these studies.…”

Section: Discussioncontrasting

confidence: 56%

“…Obtained MNa and PIC values were significantly higher than those of some other Turkish goat breeds and other goat breeds raised in different countries (17,18), while these values were lower than those of goat breeds raised in North Africa (19). The findings related to allele numbers indicated a high level of allelic richness in the breed studied.…”

Section: Discussionmentioning

confidence: 60%

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Microsatellite panels for parentage testing of Kilis goats reared in Turkey

Keskin¹,

Yılmaz²,

Gündüz³

et al. 2019

Turk J Vet Anim Sci

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The aim of this study was to develop PCR-based suitable microsatellite marker panels for paternity testing and to define pedigree errors in Kilis goats. A total of 137 head of goats were used, consisting of 118 head of kids and 19 head of possible candidate sires. A total of 392 alleles were observed in 22 microsatellite markers. Allele numbers ranged between 12 (SRCRSP7) and 24 (BM1818, INRA0023, and SRCRSP15) with an average of 17.82. The mean value of the effective allele numbers was obtained as 9.44. The overall polymorphic information content value was quite high (0.88). The overall observed (Ho) and expected heterozygosity (He) values for all studied loci were 0.89 and 0.89, respectively. Paternity test panels for the Kilis goat population studied were created based on individual probability of exclusion of microsatellites in multiplex groups. Combined probability of exclusion (CPE) values for different panels ranged between 0.745 (Panel-1) and 0.9999 , while the combined identification probability (CPI) values were obtained in the range of 9.81 × 10 -3 (Panel-1) to 6.96 × 10 -21 . As a result of this study, it can be stated that panels with 0.999 CPE values can be used at the most reasonable cost and with high reliability in paternity tests to be performed in Kilis goats, which can be a reference for other populations.

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Section: Discussioncontrasting

confidence: 56%

Section: Discussionmentioning

confidence: 60%

Microsatellite panels for parentage testing of Kilis goats reared in Turkey

Keskin¹,

Yılmaz²,

Gündüz³

et al. 2019

Turk J Vet Anim Sci

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“…Although lower than H E (0.74), this value of H O is similar to that reported for other Italian or foreign breeds [40,41,44]. Higher H E values were reported in other cases [20,37,38]. The presence of private alleles (i.e., alleles present in one breed and absent in the others) were observed in all three populations/breeds, but were about 5-fold more abundant in FAC (25 in FAC, 4 in CAM and 5 in SAA).…”

Section: Genetic Variationsupporting

confidence: 89%

“…After adopting the rarefaction methodology [24], the mean allelic richness ranged from 4.36 (CAM) to 5.17 (FAC) in a sample size of eight individuals. Lower allelic diversity was found in many local goat breeds [37][38][39][40], but higher MNA values were reported in both Italian [41][42][43] and foreign [20,44] breeds. FAC had higher observed heterozygosity compared to the cosmopolitan breeds, with H O of 0.68.…”

Section: Genetic Variationmentioning

confidence: 99%

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Molecular Identification of the “Facciuta Della Valnerina” Local Goat Population Reared in the Umbria Region, Italy

Ceccobelli

Lasagna

Demir

et al. 2020

Animals

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Italy holds important genetic resources of small ruminant breeds. By distinguishing goat breeds at the DNA level, certification of products from specific breeds can be valorized. The aim of this study was to establish the genetic identity of Facciuta della Valnerina, a local goat population of Italy, compared with the cosmopolitan breeds, Saanen and Camosciata delle Alpi, reared in the same geographic area. A total of 116 microsatellite alleles ranging from 4 to 13 were detected at 16 loci in the three goat populations/breeds. A total of 23 private alleles with frequencies lower than 0.3 were detected in the Facciuta della Valnerina population. The mean numbers of alleles were 6.67, 4.58, and 4.92 in Facciuta della Valnerina, Camosciata delle Alpi, and Saanen, respectively. The expected heterozygosity ranged from 0.20 to 0.86. Most loci were highly polymorphic and informative (polymorphic information content ≥0.50). Factorial correspondence analysis and principal components analysis revealed very clear separation between Facciuta della Valnerina and the two reference goat breeds. Reducing the number of markers from 16 to 12 (on the basis of polymorphic information content and the number of alleles) still allowed us to distinguish the local population, indicating that microsatellite markers are capable of discriminating local livestock breeds at a low cost.

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