Genetic characterization of Golden mahseer (Tor putitora) populations using mitochondrial DNA markers

Abstract

Golden Mahseer (Tor putitora) is an economically important fish of India and Southeast Asia. The present study examined the genetic variations between seven geographically isolated populations of T. putitora using Cyt b (Cytochrome b) and ATPase6/8 gene sequences of mitochondrial DNA. Analysis of 133 sequences of Cyt b (1141 bp) and 130 sequences of ATPase6/8 gene (842 bp) revealed 47 and 44 haplotypes, respectively. The estimated haplotype and nucleotide diversity was high in River Jia Bhoreli (Bhalukpong) population (h = 1.00000, π = 0.007121 for Cyt b and h = 0.90441 π = 0.004867 for ATPase6/8). Results of AMOVA indicated that majority of the genetic variations in both genes were due to variation among populations (60.79% for Cyt b and 51.41% for ATPase6/8 gene). The pairwise F(ST) comparison and neighbor-joining tree revealed high genetic divergence of River Jia Bhoreli population from other populations. The understanding of genetic variations of T. putitora populations will play a key role in conservation and management of this endangered fish species.

Evaluation of the effect of longitudinal connectivity in population genetic structure of endangered golden mahseer, Tor putitora (Cyprinidae), in Himalayan rivers: Implications for its conservation

“…We detected 83 haplotypes from 333 GM sequences. Of these, 47 haplotypes had been reported by Sati et al (2015) [22] from seven rivers, and only two haplotypes (H1 and H2) of her study were shared with our data (Fig 2). https://doi.org/10.1371/journal.pone.0234377.g001…”

“…Our data supplement those relating to seven other Indian rivers. In a previous study, 134 GM individuals were sequenced into 47 distinct haplotypes [22]. Strikingly, only two (H1 and H2) of those haplotypes were found in our study.…”

“…Sequences of GM from seven other different Indian rivers, namely the Ravi (Basoli, Jammu & Kashmir, n = 22), Beas (Joginder Nagar, Himachal Pradesh, n = 16), Satluj (Bhakara, Himachal Pradesh, n = 21), Chenab (Anji, Jammu & Kashmir, n = 21), Kosi (Ram Nagar, Uttarakhand, n = 19), Jia Bhoreli (Bhalukpong, Assam, n = 17), and Walwan dam on the Indrayani (Lonavala, Maharashtra, n = 16) were taken from GenBank to determine the inclusive mtDNA structure and to carry out network analysis [22]. We detected 83 haplotypes from 333 GM sequences.…”

“…The distribution range and population biology have been done from Indian rivers, but detail population genetic studies of GM is still lacking [1,2,8,[14][15][16]. Despite this, no genetic assessment has been carried out in the Ganga and Yamuna river; however, a few genetic studies have been conducted in other rivers [17][18][19][20][21][22]. Barriers created by humans may have the potential to cause genetic differentiation and structuring in populations that were previously continuous [23,24].…”

De novo assembly and characterization of tissue-specific transcriptome in the endangered golden mahseer, Tor putitora

“…Due to its ecological and economic importance particularly in the Himalayan regions, several attempts were made to propagate and rehabilitate the species in rivers and lakes by artificial breeding using sexually matured fishes from wild ( Pandey et al, 1998 ). Assessment of genetic vigor and population genetic variability was carried out using mitochondrial gene and microsatellite markers in geographically isolated populations from the Ganga, the Indus, and the Brahmaputra basins ( Sati et al, 2015 ). To study the genetic profiles, polymorphic microsatellite markers from a partial genomic library were identified ( Sahoo et al, 2013 ).…”