Genetic characterization of H1N1, H1N2 and H3N2 swine influenza virus in Thailand

Chutinimitkul, Salin; Thippamom, Nattakarn; Damrongwatanapokin, Sudarat; Payungporn, Sunchai; Thanawongnuwech, Roongroje; Amonsin, Alongkorn; Boonsuk, Pitirat; Sreta, Donruethai; Bunpong, Napawan; Tantilertcharoen, Rachod; Chamnanpood, Pornchai; Parchariyanon, Sujira; Theamboonlers, Apiradee; Poovorawan, Yong

doi:10.1007/s00705-008-0097-7

Cited by 41 publications

(33 citation statements)

References 22 publications

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“…The HA1 subunit includes the globular head and contains fi ve major antibody binding sites, namely Sa, Sb, Ca1, Ca2 and Cb. [17][18][19] A substitution at residue 171 (K171E) occurred in the Ca antigenic site in A/Delhi/NIV3610/2009 isolate, the substitution at residue 208 (L208I) which is located in the Ca site present in the A/Che/NIV658/2009 strain. A substitution at residue 240 (Q240R) also occurred in the Ca antigenic site of two different strains like A/Blore/NIV236/2009 and A/Jalna /NIV9436/2009 isolates.…”

Section: Discussionmentioning

confidence: 99%

Genetic analysis of HA gene of pandemic H1N1 2009 influenza viruses circulating in India

Gunasekaran

Krishnasamy

Arunagiri

et al. 2012

Indian Journal of Medical Microbiology

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The H1N1 2009 influenza pandemic took the health care workers by surprise in spite of warning about influenza pandemic. Influenza A virus has the ability to overcome immunity from previous infections through the acquisition of genetic changes by shift or drift. Thus, understanding the evolution of the viruses in human is important for the surveillance and the selection of vaccine strains. A total of 23 pandemic A/H1N1 2009 viral HA gene sequences were downloaded from NCBI submitted during March and May 2010 by NIV and were analysed. Along with that the vaccine strain A/California/07/2009 was also downloaded from NCBI. All the sequences were used to analyse the evolution of the haemagglutinin (HA) by phylogenetic analysis. The HA gene could be divided into four groups with shift from 1 to lV revealing that the HA genes of the influenza A viruses evolved in a sequential way, in comparison to vaccine strain A/California/07/2009. Amino acid sequence analysis of the HA genes of the A/H1N1 2009 isolates, revealed mutations at positions 100, 220 and additional mutations in different positions 114, 171, 179, 190, 208, 219, 222, 239, 240, 247, 251, 260 and 285 .The mutations identified showed the adaptation of the new virus to the host that could lead to genetic changes inherent to the virus resulting in a reassortant which could be catastrophic, hence continuous monitoring of strains is mandatory.

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Section: Discussionmentioning

confidence: 99%

Genetic analysis of HA gene of pandemic H1N1 2009 influenza viruses circulating in India

Gunasekaran

Krishnasamy

Arunagiri

et al. 2012

Indian Journal of Medical Microbiology

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“…In Asia, the situation is even more complex as, in addition to the above viruses, Eurasian avian-like H1N1 SIVs are present (1,6,7,10).…”

Section: Discussionmentioning

confidence: 99%

“…the surface glycoproteinshaemagglutinin (HA) and neuraminidase (NA). Three SIV subtypes: H1N1, H3N2, and H1N2 are currently circulating throughout pig populations worldwide (1,4,6,10,22,31,32).…”

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confidence: 99%

First Isolation of the H₁N₂ Swine Influenza Virus in Polish Pig Farm

Markowska-Daniel

Wierzchosławski²,

Urbaniak

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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The paper describes an outbreak of swine influenza, which was the first H1N2 outbreak confirmed in pig farm in Poland. The outbreak occurred in October 2011 in the farrows to finish farm, first in the fattening units, and subsequently in the reproduction and weaning sectors. Samples of the lungs taken from dead gilts were tested by the use of real time and multiplex PCR, sequencing, and virus isolation methods. The amplification of the genetic material extracted from the lungs confirmed the presence of the M1 gene sequence of type A influenza virus. Using multiplex PCR, the bands of 241 bp, typical for HA1 human-like type, and of 791 bp, typical for NA2, were demonstrated. The new isolates of swine influenza virus, named A/swine/Poland/15817/2011, were obtained in embryonated SPF chicken eggs and MDCK cells. Phylogenetic analysis of the HA and NA genes of the isolate revealed that the virus is of human swine linkage closely related to European human-like viruses. On the basis of such results, it can be stated that A/Swine/Poland/15817/2011 is a new swine influenza virus, representing the H1N2 subtype strain, which emerged in Poland.

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“…Swine can be infected with multiple influenza subtypes and may serve as ''mixing vessels'' for mixed infections and potential genetic reassortment among avian, human, and porcine influenza viruses (Kida et al, 1994;Ito et al, 1998). Currently, H1N1, H1N2, and H3N2 influenza viruses are the main subtypes cocirculating in swine populations worldwide (Yu et al, 2007;Chutinimitkul et al, 2008;Van Reeth et al, 2008). H5 and H9 avian influenza infections were also detected in swine in China (Ninomiya et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Exposure to Swine H1 and H3 and Avian H5 and H9 Influenza a Viruses Among Feral Swine in Southern China, 2009

Luo

Dong

et al. 2013

Journal of Wildlife Diseases

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ABSTRACT:Swine play an important role in the disease ecology of influenza. Swine may provide the potential for mixed infections and genetic reassortment between avian, human, and porcine influenza viruses. We investigated the prevalence of antibodies to swine H1 and H3 influenza viruses and avian H5 and H9 influenza viruses in feral swine in southern China. Serum samples were collected from 31 feral swine harvested in 2009 in southern China. Of 31 serum samples tested, 14 (45%) had detectable antibody to H1 influenza virus and 23 (74%) were positive for H3 subtype. The antibody prevalence against both the swine H1 virus and the swine H3 virus was 45% (14/31). Five samples were reactive with both H1 and N1 subtype viruses, suggesting exposure to H1N1 viruses. All the sera tested were negative for avian H5 and H9 influenza viruses. Further investigations of influenza virus exposure of feral swine are needed to clarify their role in influenza ecology.

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Genetic characterization of H1N1, H1N2 and H3N2 swine influenza virus in Thailand

Cited by 41 publications

References 22 publications

Genetic analysis of HA gene of pandemic H1N1 2009 influenza viruses circulating in India

Genetic analysis of HA gene of pandemic H1N1 2009 influenza viruses circulating in India

First Isolation of the H₁N₂ Swine Influenza Virus in Polish Pig Farm

Exposure to Swine H1 and H3 and Avian H5 and H9 Influenza a Viruses Among Feral Swine in Southern China, 2009

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Genetic characterization of H1N1, H1N2 and H3N2 swine influenza virus in Thailand

Cited by 41 publications

References 22 publications

Genetic analysis of HA gene of pandemic H1N1 2009 influenza viruses circulating in India

Genetic analysis of HA gene of pandemic H1N1 2009 influenza viruses circulating in India

First Isolation of the H1N2 Swine Influenza Virus in Polish Pig Farm

Exposure to Swine H1 and H3 and Avian H5 and H9 Influenza a Viruses Among Feral Swine in Southern China, 2009

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First Isolation of the H₁N₂ Swine Influenza Virus in Polish Pig Farm