Genetic characterization of HIV-1 epidemic in Anhui Province, China

Zhang, Dong; Wu, Jianjun; Shen, Yuelan; Dai, Sheying; Wang, Xiaolin; Xing, Hui; Lin, Juan; Han, Jingwan; Li, Jingyun; Qin, Yizu; Liu, Yongjian; Miao, Lifeng; Su, Bin; Li, Hanping; Li, Lin

doi:10.1186/s12985-020-1281-y

Cited by 25 publications

(20 citation statements)

References 27 publications

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“…The predominant CRFs in our study were similar to other provinces in China 26 , 35 , 36 , but different from those in southeast Asian countries 31 – 33 , United States 29 , Canada and European countries 37 – 41 . The newly detected CRFs in our study had been circulating in the northeast and middle and southern parts of China since 2013 42 – 46 , which concurred with the results from our temporal geographical analysis.…”

Section: Discussionsupporting

confidence: 91%

HIV-1 subtype diversity and transmission strain source among men who have sex with men in Guangxi, China

Chen

Shen

Feng

et al. 2021

Sci Rep

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With the rapid increase in HIV prevalence of men who have sex with men (MSM) in recent years and common human migration and travelling across different provinces in China, MSM are now finding it easier to meet each other, which might contribute to local HIV epidemics as well as fueling cross-province transmission. We performed a cross-sectional survey in 2018–2019 to investigate the current HIV subtype diversity and inferred HIV strain transmission origin among MSM in Guangxi province, China based on a phylogenetic analysis. Based on 238 samples, we found that the HIV-1 subtype diversity was more complicated than before, except for three major HIV subtypes/circulating recombinant forms (CRFs): CRF07_BC, CRF01_AE, CRF55_01B, five other subtypes/CRFs (CRF59_01B, B, CRF08_BC, CRF67_01B, CRF68_01B) and five unique recombinant forms (URFs) were detected. In total, 76.8% (169/220) of samples were infected with HIV from local circulating strains, while others originated from other provinces, predominantly Guangdong and Shanghai. The high diversity of HIV recombinants and complicated HIV transmission sources in Guangxi MSM indicates that there has been an active sexual network between HIV positive MSM both within and outside Guangxi without any effective prevention. Inter-province collaboration must be enforced to provide tailored HIV prevention and control services to MSM in China.

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Section: Discussionsupporting

confidence: 91%

HIV-1 subtype diversity and transmission strain source among men who have sex with men in Guangxi, China

Chen

Shen

Feng

et al. 2021

Sci Rep

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“…The genetic distance cut-off value for constructing the transmission network differs among sequences belonging to different subtypes. 34 In the present study, genetic distances ≤0.013 and ≤0.015 were set as cut-off values for CRF07_BC and CRF01_AE, CRF55_01B, or CRF59_01B, respectively. The results showed that most (70.4% for CRF01_AE, 63.6% for CRF07_BC, and 55.8% for CRF55_01B) of the MSM in transmission networks were concentrated at ages below 35 years old.…”

Section: Discussionmentioning

confidence: 99%

HIV-1 Drug Resistance and Genetic Transmission Networks Among MSM Failing Antiretroviral Therapy in South China 2014–2019

Lan¹,

Deng²,

Li³

et al. 2021

IDR

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Background Guangdong, located in South China, is one of the areas heavily affected by HIV-1 in China. The transmission of HIV-1 among men who have sex with men (MSM) has gradually been increasing in Guangdong. Objective To investigate the characteristics of the HIV-1 drug resistance, and genetic transmission networks in MSM with antiretroviral therapy (ART) failure from 2014 to 2019 in Guangdong. Methods HIV-1 pol gene sequences were amplified. An online subtyping tool was used to determine the genotype, and a maximum likelihood phylogenetic tree was reconstructed to confirm the genotype results. The Stanford University HIV Drug Resistance Database was used to analyse the sequences of drug resistance mutations (DRMs) and drug resistance profiles. A pairwise Tamura-Nei 93 genetic distance-based method was used to analyse the genetic transmission networks. Results Of 393 sequences isolated from HIV-infected MSM with ART failure, CRF01_AE (47.3%), CRF07_BC (21.4%) and CRF55_01B (21.4%) were the top three strains. 55.2% individuals harboured NRTI DRMs, whereas 67.4% carried NNRTI DRMs. 96.8% cases harboured mutations resistance to NRTIs or NNRTIs at high-level. The most common DRMs were M184I/V (42.2%), followed by V179D/E (37.9%) and K65R (27.2%). Of the subtype B sequences, no sequence fell into a cluster. Of the CRF01_AE, CRF55_01B, and CRF59_01B sequences, 14.5%, 61.9%, and 33.3% fell into clusters, respectively. Of the CRF07_BC sequences, 39.3% fell into clusters. The majority of MSM in transmission networks were concentrated at age below 35 years old, with multiple links. Moreover, approximately 54.8% of MSM had more than 2 potential transmission partners. Conclusion Drug resistance mutations more frequently occurred in NNRTIs among MSM with ART failure in Guangdong Province. Transmission network analysis revealed a complex transmission pattern, and more attention should be given to younger HIV-1-infected MSM with multiple links.

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“…RIP and jpHMM tools in HIV database were used to identify the recombinant strains. 11 A phylogenetic tree was constructed by the neighborjoining method based on the Kimura 2-parameter model with 10 0 0 bootstrap replicates in MEGA6 software to determine the subtypes of the sequences. 12…”

Section: Hiv-1 Subtypingmentioning

confidence: 99%

Molecular surveillance of HIV-1 newly diagnosed infections in Shenzhen, China from 2011 to 2018

Zhang

Zheng

et al. 2021

Journal of Infection

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Objectives: Shenzhen is suffering severe HIV epidemic. No systematic surveillance on high risk populations, HIV genetic diversity, transmitted drug resistance (TDR) and molecular transmission clusters (MTCs) have been reported yet. In this study, we described them based on newly diagnosed HIV positive cases from 2011 to 2018 in Shenzhen city, China. Methods: Plasma samples of newly reported HIV positive cases in Shenzhen, China were collected from 2011 to 2018. The HIV pol gene was amplified and sequenced for subtyping, genetic characterization, TDR and phylogenetic analysis. Demographic and risk characteristics associated with transmitted drug resistance-associated mutations (TDRAMs) and MTCs were explored by using logistic regression analyses. Results: 10,378 HIV pol sequences were successfully obtained from newly diagnosed patients with available background information. The most prevalent HIV-1 subtype was CRF07_BC (40.92%). CRF07_BC, CRF55_01B and URFs increased across years. Total TDR was 6.02% during 2011 to 2018. CRF01_AE, CRF08_BC, CRF55_01B and subtype B were more likely to be associated with TDRAMs than CRF07_BC. 4460 (42.98%) patients were infected with strains included in MTCs. Patients younger than 30 and over 50 years were more likely to cluster. Conclusions: The prevalence of HIV-1 drug resistance and molecular transmission clusters in Shenzhen should raise a high alert. Interventions targeting on patients with strains locating in MTCs should be considered to improve prevention effect in Shenzhen.

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Genetic characterization of HIV-1 epidemic in Anhui Province, China

Cited by 25 publications

References 27 publications

HIV-1 subtype diversity and transmission strain source among men who have sex with men in Guangxi, China

HIV-1 subtype diversity and transmission strain source among men who have sex with men in Guangxi, China

HIV-1 Drug Resistance and Genetic Transmission Networks Among MSM Failing Antiretroviral Therapy in South China 2014–2019

Molecular surveillance of HIV-1 newly diagnosed infections in Shenzhen, China from 2011 to 2018

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