Genetic Characterization of Landraces and Varieties using Functional Microsatellite Markers in Eleusine Coracana (L.)

Abstract

About 3515 genomic and EST sequences from the public database were mined in-silico for microsatellite repeats containing di-, tri-, tetra-and Penta-nucleotide repeat motifs and 3334 primer pairs were designed. One hundred primers were synthesized and validated in Eleusine coracana species, and the polymorphic microsatellites were used for characterizing 32 local land races and commercial varieties collected from five different districts in Karnataka. The studies were focused on estimating genetic parameters of the markers, genetic diversity and population structure.