Genetic characterization of micropropagated Iris germanica varieties as assessed by RAPD and ISSR markers

Petolescu, C.; Simona, Lapadatescu; Alexandru, Lazar; Bala, Manju

doi:10.1016/j.copbio.2013.05.383

Cited by 3 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Iris pseudacorus and I. germanica genetic profiling was studied before using RAPD 50 . Upon comparing the genetic polymorphism among regenerated and mother plants of I. germanica using ISSR and RAPD techniques, it was found that ISSR primers generated a higher number of clearly detected bands than RAPD 51 …”

Section: Discussionmentioning

confidence: 99%

Discrimination of common Iris species from Egypt based on their genetic and metabolic profiling

Okba

Abdel-Baki

Khaleel

et al. 2020

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction Irises have been medicinally used in Ancient Egyptians, Anatolian, Chinese, British and Irish folk medicine. They are also well‐known ornamental plants that have economic value in the perfume industry. The main obvious diagnostic difference between the different species is based on the morphology of the flowers. The flowering cycle is very short as well as the persistence of the fully opened flowers extends for a few days only. Moreover, the climatic conditions significantly causes fluctuation in their blooming time from year to year. This makes the morphological discrimination very difficult. The discrimination of different iris species is of a great importance, as each species is reported to possess different folk medicinal activities. Objectives Finding genetic and metabolic markers for differentiation between Iris confusa Sealy (Subgen. Limniris Sect. Lophiris), I. pseudacorus L. (Subgen. Limniris Sect. Limniris) and I. germanica L. (Subgen. Iris Sect. Iris) on levels other than traditional taxonomic features. Material and Methods Inter‐simple sequence repeat (ISSR) and gas chromatography–mass spectrometry (GC–MS) analyses were performed. Results The highest similarity was found between I. pseudacorus L. and I. germanica L. and the least similarity was between I. confusa Sealy and I. pseudacorus L. The metabolic profiling of the leaves confirmed genetic profiling discriminating I. confusa from the other two species. The primary metabolites of the underground parts showed clear discrimination between the three species. Conclusions This study represents the sole complete map for distinguishing the three Iris species on genetic and metabolic bases.

show abstract

Section: Discussionmentioning

confidence: 99%

Discrimination of common Iris species from Egypt based on their genetic and metabolic profiling

Okba

Abdel-Baki

Khaleel

et al. 2020

Phytochemical Analysis

View full text Add to dashboard Cite

show abstract

“…The capacity of callus induction of different explants is very diverse, depending on their genotype and growth status [27]. Stem tips, leaf bases, oral organs, rhizomes, and roots are often used for callus regeneration research with Iris species [14][15][16][28][29][30][31]. Two explants, hypocotyls and roots, were investigated in this research, and there was no signi cant difference in callus induction rate between the two types of explants, 75% and 73.33%, respectively.…”

Section: Discussionmentioning

confidence: 99%

An efficient in vitro regeneration system via callus from hypocotyl and root of Iris laevigata

Li¹,

Fan²,

Fu³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Iris laevigata is an ornamental plant with strong cold resistance. However, its low reproductive capacity limits its landscape applications. The I. laevigata wild genetic resources also need to be protected. In order to develop an effective regeneration system, the optimum agar concentration for the induction medium was determined. Two explants (hypocotyl and root) were then cultured on medium containing different concentrations of plant growth regulator (PGR). In addition, three antibiotics were evaluated for controlling endophyte contamination. Results The highest induction rate (75.00%) was obtained from hypocotyl explants on Murashige and Skoog salt mixture (MS) medium containing 6-benzylaminopurine (6-BA) 0.5 mg L-1 + 2,4-Dichlorophenoxyacetic acid (2,4-D) 1.0 mg L-1 + 1-naphthylacetic acid (NAA) 0.4 mg L-1. The medium containing 6-BA 0.5 mg L-1 + 2,4-D 0.5 mg L-1 + NAA 0.2 mg L-1 achieved the greatest multiplication rate (73.33%). Media containing indole-3-butyric acid (IBA) 0.5 mg L-1 + 6-BA 1.5 mg L-1 + NAA 1.0 mg L-1 achieved the highest differentiation rate (39.72%) for hypocotyl induced calli. Medium containing 6-BA 2.0 mg L-1 + NAA 0.4 mg L-1 + kinetin (KT) 1.0 mg L-1 resulted in the highest differentiation rate (49.52%) for root induced calli. One hundred mg L-1 penicillin G resulted in the optimal rate for reducing endophyte contamination. Conclusions The research determined the optimum PGR concentrations for inducting and multiplying I. laevigata calli from hypocotyl and root explants and a satisfactory means for control endophyte contamination. This research will result in the efficient and reliable reproduction of I. laevigata for landscape applications, genetic development of new Iris varieties, and preservation of the wild genetic material.

show abstract

“…The genus Iris is a winter-hardy, herbaceous perennial floral plant that includes over 300 species, distributed mostly across the northern temperate zone (Cerasela et al, 2014). As one of the important plant sources in urban landscaping, the genus Iris can make up for the deficiency of trees that grow slowly to achieve better effects of landscaping within a short term.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies of the genus Iris are mainly concentrated in pollen morphology (Oybak Dönmez and IşIk, 2008;Pinar and Dönmez, 2000), cytogenetic karyotype analysis (Karihaloo et al, 1993) and genetic diversity analysis (Azimi et al, 2012;Cerasela et al, 2014). The researches on plant resistance to salt stress and heavy metal stress also have been partly studied (Bai et al, 2008;Han et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of drought resistance in Iris germanica L. based on subordination function and principal component analysis

Wei

Qin

et al. 2017

Emir J Food Agric

View full text Add to dashboard Cite

Drought is one of the major environment stresses that have a wide range of impact on plants. In this study, seven physiological indexes including the content of soluble protein (SP), chlorophyll (Chl) and malondialdehyde (MDA), superoxide dismutase (SOD) and peroxidase (POD) activities, leaf relative water content (RWC), rate of water loss (RWL) from excised leaves were measured in leaves of Iris germanica before and after the drought treatment. It was found that the content of MDA and SP, POD and SOD activity increased, while RWL and RWC decreased in response to drought stress. Based on the subordinate function values of seven physiological indexes, seven single indexes were transformed into three principal components namely damage degree, active oxygen removal ability and moisture condition and the composite score (F value) of each iris variety was calculated by principal component analysis (PCA). Based on the F values, 10 iris cultivars could be divided into three groups by cluster analysis (CA): drought-resistance (2 varieties), medium drought-resistance (5 varieties), and low drought-resistance (3 varieties). Meanwhile, optimum regression equation was constructed. Therefore, this work provides a comprehensive and reliable method for evaluating drought resistance in the varieties of Iris germanica.

show abstract

Genetic characterization of micropropagated Iris germanica varieties as assessed by RAPD and ISSR markers

Cited by 3 publications

References 0 publications

Discrimination of common Iris species from Egypt based on their genetic and metabolic profiling

Discrimination of common Iris species from Egypt based on their genetic and metabolic profiling

An efficient in vitro regeneration system via callus from hypocotyl and root of Iris laevigata

Evaluation of drought resistance in Iris germanica L. based on subordination function and principal component analysis

Contact Info

Product

Resources

About