Genetic characterization of Moniliophthora perniciosa from Ecuador and in vitro sensitivity to compost tea

Maridueña-Zavala, María Gabriela; Freire-Peñaherrera, Andrea; Espinoza-Lozano, Fernando; Villavicencio-Vásquez, Mirian Elizabeth; Jimenez-Feijoó, María Isabel; Cevallos-Cevallos, Juan Manuel

doi:10.1007/s10658-019-01714-1

Cited by 5 publications

(5 citation statements)

References 41 publications

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“…The compost tea significantly inhibited the growth of all M. roreri isolates at concentrations of 1 % or above, reaching full inhibition at concentrations of 4.5 % or greater. The compost tea concentrations effective against M. roreri were lower than the 5 to 20 % needed for the control of other fungal pathogens such as Alternaria solani 23 , Alternaria alternate, Botrytis cinereal, and Pyrenochata lycopersici 24 but higher than the 2.5 % required to inhibit the growth of M. perniciosa 25 , suggesting an intermediate to high sensitivity of M. roreri to compost tea. Further research is needed to assess the sensitivity of M. roreri to compost tea in the field.…”

Section: Discussionmentioning

confidence: 80%

Pathogenicity of Moniliophthora roreri isolates from selected morphology groups in harvested cacao pods and in vitro sensitivity to compost tea.

Feijoo

Cevallos-Cevallos

2021

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Moniliopthora roreri is the frosty pod rot disease (FPD) and one of the most devastating cacao pathogens worldwide. However, M. roreri pathogenicity on harvested cacao pods and sensitivity to compost tea have not been fully described. Monosporic cultures of M. roreri from different morphology groups were obtained. The isolates’ pathogenicity was tested by inoculation onto harvested cacao pods, and symptoms were evaluated at 3-day intervals during 16 days before estimating the area under the disease progress curve (AUDPC). The sensitivity of M. roreri to compost tea was evaluated on potato dextrose agar (PDA) amended with 1 to 5 % compost tea. All morphology groups could infect harvested cacao pods during the 16 days with a disease severity index abode 75 %. Compost tea completely inhibited the growth of M. roreri when used at 4.5 % or higher. Results suggest a shortened biotrophic phase during the infection in harvested pods and a medium to high sensitivity of M. roreri to compost tea.

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Section: Discussionmentioning

confidence: 80%

Pathogenicity of Moniliophthora roreri isolates from selected morphology groups in harvested cacao pods and in vitro sensitivity to compost tea.

Feijoo

Cevallos-Cevallos

2021

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“…The most cited papers in the SRDB formed five clusters of co-citations ( Figure 2 d): cluster 1 contained papers discussing, among other results, the population structure or subpopulation of M. perniciosa and M. roreri pathogens [ 14 , 44 , 45 , 46 ] and Phytophthora spp. [ 47 , 48 ]; cluster 2 contained papers discussing M. perniciosa effector proteins [ 49 , 50 , 51 ]; cluster 3 contained papers based on molecular markers discussing M. perniciosa genetic diversity, which is similar to cluster 1 [ 52 , 53 , 54 ]; cluster 4 contained studies characterizing and evaluating M. perniciosa proteins interacting with T. cacao [ 55 , 56 , 57 ]; and cluster 5 contained molecular responses of M. roreri during its interaction with T. cacao [ 58 , 59 ].…”

Section: Resultsmentioning

confidence: 99%

“…The molecular marker analysis category was formed by papers that developed or used existing molecular markers to investigate pathogen genomes. Molecular markers for population studies were developed for M. perniciosa [ 53 , 63 , 64 ], M. roreri [ 46 , 65 ] and Phytophthora spp. [ 46 , 66 , 67 , 68 ].…”

Section: Resultsmentioning

confidence: 99%

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Genomic and Pathogenicity Mechanisms of the Main Theobroma cacao L. Eukaryotic Pathogens: A Systematic Review

et al. 2023

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A set of diseases caused by fungi and oomycetes are responsible for large losses in annual world cocoa production. Managing the impact caused by these diseases is very complex because a common solution has yet to be found for different pathogens. In this context, the systematic knowledge of Theobroma cacao L. pathogens’ molecular characteristics may help researchers understand the possibilities and limitations of cocoa disease management strategies. This work systematically organized and summarized the main findings of omics studies of T. cacao eukaryotic pathogens, focusing on the plant–pathogen interaction and production dynamics. Using the PRISMA protocol and a semiautomated process, we selected papers from the Scopus and Web of Science databases and collected data from the selected papers. From the initial 3169 studies, 149 were selected. The first author’s affiliations were mostly from two countries, Brazil (55%) and the USA (22%). The most frequent genera were Moniliophthora (105 studies), Phytophthora (59 studies) and Ceratocystis (13 studies). The systematic review database includes papers reporting the whole-genome sequence from six cocoa pathogens and evidence of some necrosis-inducing-like proteins, which are common in T. cacao pathogen genomes. This review contributes to the knowledge about T. cacao diseases, providing an integrated discussion of T. cacao pathogens’ molecular characteristics, common mechanisms of pathogenicity and how this knowledge is produced worldwide.

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“…The typical symptoms include abnormal shoots that display stem swellings, bud proliferation, and vegetative brooms, which inspired the name witches’ broom disease in cocoa [ 5 ]. The results of recent studies from the major cocoa-producing provinces of Ecuador showed that Moniliophthora species possess high morphological and genetic diversity [ 6 , 7 , 8 , 9 ]. For this reason, some alternatives such as cacao agroforestry systems [ 1 ] and biological controls using products based on endophytic organisms, including the combined application of natural or chemical products [ 10 ], have been used to reduce the impact of diseases, minimize the utilization of fungicides, and improve the yield of cocoa production.…”

Section: Introductionmentioning

confidence: 99%

Chemical Characterization of Trichoderma spp. Extracts with Antifungal Activity against Cocoa Pathogens

et al. 2023

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Ecuador is one of the major cocoa producers worldwide, but its productivity has lately been affected by diseases. Endophytic biocontrol agents have been used to minimize pathogenic effects; however, compounds produced by endophytes are minimally understood. This work presents the chemical characterization of the Trichoderma species extracts that proved inhibition against cocoa pathogens. Solid–liquid extraction was performed as a partitioning method using medium with the fungal mycelia of Trichoderma reesei (C2A), Trichoderma sp. (C3A), Trichoderma harzianum (C4A), and Trichoderma spirale (C10) in ethyl acetate individually. The extract of T. spirale (C10) exhibited the growth inhibition (32.97–47.02%) of Moniliophthora perniciosa at 10 µg/mL, while a slight stimulation of Moniliophthora roreri was shown by the extracts of T. reesei (C2A) and T. harzianum (C4A) at higher concentrations. The inhibitory activity could be related to alkaloids, lactones, quinones, flavonoids, triterpenes, and sterols, as indicated by chemical screening and antifungal compounds, such as widdrol, β-caryophyllene, tyrosol, butyl isobutyrate, sorbic acid, palmitic acid, palmitelaidic acid, linoleic acid, and oleic acid, which were identified by gas chromatography–mass spectrometry (GC-MS). The results showed that the extracts, particularly T. spirale (C10), have the potential as biocontrol agents against witches’ broom disease; however, further studies are needed to confirm their effectiveness.

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Genetic characterization of Moniliophthora perniciosa from Ecuador and in vitro sensitivity to compost tea

Cited by 5 publications

References 41 publications

Pathogenicity of Moniliophthora roreri isolates from selected morphology groups in harvested cacao pods and in vitro sensitivity to compost tea.

Pathogenicity of Moniliophthora roreri isolates from selected morphology groups in harvested cacao pods and in vitro sensitivity to compost tea.

Genomic and Pathogenicity Mechanisms of the Main Theobroma cacao L. Eukaryotic Pathogens: A Systematic Review

Chemical Characterization of Trichoderma spp. Extracts with Antifungal Activity against Cocoa Pathogens

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