Genetic Characterization of Non-Lymphogranuloma venereum Chlamydia trachomatis Indicates Distinct Infection Transmission Networks in Spain

Piñeiro, Luis; Villa, Laura; Salmerón, Paula; Macià, María D.; Otero, Luís; Vall-Mayans, Martí; Milagro, Ana; Bernal, Samuel; Manzanal, Ayla; Ansa, Iñigo; Cilla, Gustavo

doi:10.3390/ijms24086941

Cited by 3 publications

(1 citation statement)

References 39 publications

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“…It could be that this small proportion of strains expressing heterotypic resistance would have lower fitness or clonal dissemination. However, on the other hand, the distribution of C. trachomatis genotypes in these strains of patients with treatment failure in the present study was similar to that described in the general population without treatment failure in the same period in Spain [ 59 ]. Genotype E was the most prevalent (43.3%), followed by genotypes F, D, and G (22.4%, 13.4%, and 10.4%, respectively).…”

Section: Discussionsupporting

confidence: 87%

Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain

et al. 2023

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The aim of this multicentre project (seven hospitals across the Spanish National Health Service) was to study the phenotypic and genotypic susceptibility of C. trachomatis to the main antimicrobials used (macrolides, doxycycline, and quinolones) in isolates from patients with clinical treatment failure in whom reinfection had been ruled out. During 2018–2019, 73 clinical isolates were selected. Sixty-nine clinical specimens were inoculated onto confluent McCoy cell monolayers for phenotypic susceptibility testing. The minimum inhibitory concentration for azithromycin and doxycycline was defined as the lowest concentration associated with an at least 95% reduction in inclusion-forming units after one passage in the presence of the antibiotic compared to the initial inoculum for each strain (control). Sequencing analysis was performed for the genotypic detection of resistance to macrolides, analysing mutations in the 23S rRNA gene (at positions 2057, 2058, 2059, and 2611), and quinolones, analysing a fragment of the gyrA gene, and searching for the G248T mutation (Ser83->Ile). For tetracyclines, in-house RT-PCR was used to test for the tet(C) gene. The phenotypic susceptibility testing was successful for 10 isolates. All the isolates had minimum inhibitory concentrations for azithromycin ≤ 0.125 mg/L and for doxycycline ≤ 0.064 mg/L and were considered sensitive. Of the 73 strains studied, no mutations were found at positions T2611C or G248T of the gyrA gene. We successfully sequenced 66 isolates. No macrolide resistance-associated mutations were found at positions 2057, 2058, 2059, or T2611C. None of the isolates carried the tet(C) gene. We found no evidence for genomic resistance in this large, clinically relevant dataset.

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Section: Discussionsupporting

confidence: 87%

Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain

et al. 2023

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Genetic characterisation of lymphogranuloma venereum in Spain: a multicentre study

Salmerón,

Serra-Pladevall,

Vall-Mayans

et al. 2024

Sex Transm Infect

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IntroductionLymphogranuloma venereum (LGV) is a sexually transmitted infection caused byChlamydia trachomatisgenotypes L1–L3. A combination of techniques with high discriminatory capacity such as multilocus sequence typing (MLST) and the analysis of theompAgene may be useful to determine the greater penetration of certain strains in transmission networks and their relationship with certain tropisms.AimThe aim of this study was to investigate the molecular epidemiology of LGV isolates from different regions of Spain.MethodsGenetic characterisation of LGV isolates detected in six hospitals from Spain between 2018 and 2019 was performed. MLST (five variable regions:hctB, CT058, CT144, CT172 andpbpB) andompAsequence determination were used to study the LGV strains.ResultsMost of the 161 LGV isolates (93.8%) were detected in men who have sex with men (MSM). At least 43.5% of the patients presented with HIV coinfection and 53.4% were symptomatic, with proctitis being the most prevalent symptom (73.3%). Most isolates were detected in Barcelona (n=129).The distribution ofompAgenovariants was as follows: 56.1% belonged to L2, 24.3% to L2b, 5.4% to L2bV1, 4.7% to L2bV4, 4.1% to L1, 2.7% to L2b/D-Da, 2.0% to L2bV2 and 0.7% to L2bV7. MLST was successfully performed in 81 samples and 9 different sequence types (STs) were detected. TheompAand MLST combination obtained 17 different genetic profiles, with L2-ST53 and L2-ST58 being the most prevalent (29.5% and 14.1%, respectively). L1 genotype strains belonged to ST23 (n=3) and ST2 (n=3).ConclusionLGV infections were mainly found in MSM living with HIV and with proctitis. The joint analysis ofompAand MLST genetic characterisation techniques showed a high discriminatory capacity. Our findings suggest a cocirculation of L2 and L2bompAgenotypes, and with the inclusion of MLST characterisation, the most prevalent profiles wereompAgenotype L2-MLST ST53 and L2-MLST ST58.

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Clustering of Polymorphic Membrane Protein E Clade in Chlamydia trachomatis Lineages from Men who Have Sex with Men

Mitobe,

Kubota,

Kobayashi

et al. 2024

Emerg. Infect. Dis.

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Genetic Characterization of Non-Lymphogranuloma venereum Chlamydia trachomatis Indicates Distinct Infection Transmission Networks in Spain

Cited by 3 publications

References 39 publications

Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain

Phenotypic and Genotypic Antimicrobial Susceptibility Testing of Chlamydia trachomatis Isolates from Patients with Persistent or Clinical Treatment Failure in Spain

Genetic characterisation of lymphogranuloma venereum in Spain: a multicentre study

Clustering of Polymorphic Membrane Protein E Clade in Chlamydia trachomatis Lineages from Men who Have Sex with Men

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