Genetic characterization of ØVC8 lytic phage for Vibrio cholerae O1

Solís-Sánchez, Alejandro; Hernández-Chiñas, Ulises; Navarro, Armando; Mora, Javier; Xicohtencatl‐Cortes, Juan; Eslava-Campos, Carlos

doi:10.1186/s12985-016-0490-x

Cited by 17 publications

(13 citation statements)

References 47 publications

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“…However, it remained still largely unknown how the phage S-2L incorporates the base Z in its genome, especially as no gene corresponding to a DNA polymerase could be detected. This is in contrast with the situation in the phage φVC8, where a DNA polymerase of the family A has been identified 16 .…”

Section: Introductioncontrasting

confidence: 64%

How cyanophage S-2L rejects adenine and incorporates 2-aminoadenine to saturate hydrogen bonding in its DNA

et al. 2021

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Bacteriophages have long been known to use modified bases in their DNA to prevent cleavage by the host’s restriction endonucleases. Among them, cyanophage S-2L is unique because its genome has all its adenines (A) systematically replaced by 2-aminoadenines (Z). Here, we identify a member of the PrimPol family as the sole possible polymerase of S-2L and we find it can incorporate both A and Z in front of a T. Its crystal structure at 1.5 Å resolution confirms that there is no structural element in the active site that could lead to the rejection of A in front of T. To resolve this contradiction, we show that a nearby gene is a triphosphohydolase specific of dATP (DatZ), that leaves intact all other dNTPs, including dZTP. This explains the absence of A in S-2L genome. Crystal structures of DatZ with various ligands, including one at sub-angstrom resolution, allow to describe its mechanism as a typical two-metal-ion mechanism and to set the stage for its engineering.

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Section: Introductioncontrasting

confidence: 64%

How cyanophage S-2L rejects adenine and incorporates 2-aminoadenine to saturate hydrogen bonding in its DNA

et al. 2021

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“…; Solís‐Sánchez et al. ). An in vitro study revealed an over 90% decrease of the Vibrio population after 4 h of treatment with lytic bacteriophages.…”

Section: Potential Control and Prevention Measures Against Vibriosismentioning

confidence: 98%

Vibriosis in Fish: A Review on Disease Development and Prevention

et al. 2018

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Current growth in aquaculture production is parallel with the increasing number of disease outbreaks, which negatively affect the production, profitability, and sustainability of the global aquaculture industry. Vibriosis is among the most common diseases leading to massive mortality of cultured shrimp, fish, and shellfish in Asia. High incidence of vibriosis can occur in hatchery and grow-out facilities, but juveniles are more susceptible to the disease. Various factors, particularly the source of fish, environmental factors (including water quality and farm management), and the virulence factors of Vibrio, influence the occurrence of the disease . Affected fish show weariness, with necrosis of skin and appendages, leading to body malformation, slow growth, internal organ liquefaction, blindness, muscle opacity, and mortality. A combination of control measures, particularly a disease-free source of fish, biosecurity of the farm, improved water quality, and other preventive measures (e.g., vaccination) might be able to control the infection. Although some control measures are expensive and less practical, vaccination is effective, relatively cheap, and easily implemented. In this review, the latest knowledge on the pathogenesis and control of vibriosis, including vaccination, is discussed. 4INA-SALWANY ET AL.

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“…S6). Whether or not it is true for the natural hosts of S-2L and ϕVC8 is uncertain but we note that these phages are known to be lytic anyway (7, 21).…”

Section: Resultsmentioning

confidence: 91%

Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA

Czernecki

Bonhomme

Kaminski

et al. 2021

Preprint

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Cyanophage S-2L is known to profoundly alter the biophysical properties of its DNA by replacing all adenines (A) with 2-aminoadenines (Z), which still pair with thymines but with a triple hydrogen bond. It was recently demonstrated that a homologue of adenylosuccinate synthase (PurZ) and a dATP triphosphohydrolase (DatZ) are two important pieces of the metabolism of 2-aminoadenine, participating in the synthesis of ZTGC-DNA. Here, we determine that S-2L PurZ can use either dATP or ATP as a source of energy, thereby also depleting the pool of nucleotides in dATP. Furthermore, we identify a conserved gene (mazZ) located between purZ and datZ genes in Siphoviridae phage genomes, and show that it encodes a (d)GTP-specific diphosphohydrolase, thereby providing the substrate of PurZ in the 2-aminoadenine synthesis pathway. High-resolution crystal structures of S-2L PurZ and MazZ with their respective substrates provide a rationale for their specificities. The Z-cluster made of these three genes – datZ, mazZ and purZ – was expressed in E. coli, resulting in a successful incorporation of 2-aminoadenine in the bacterial chromosomal and plasmidic DNA. This work opens the possibility to study synthetic organisms containing ZTGC-DNA.

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Genetic characterization of ØVC8 lytic phage for Vibrio cholerae O1

Cited by 17 publications

References 47 publications

How cyanophage S-2L rejects adenine and incorporates 2-aminoadenine to saturate hydrogen bonding in its DNA

How cyanophage S-2L rejects adenine and incorporates 2-aminoadenine to saturate hydrogen bonding in its DNA

Vibriosis in Fish: A Review on Disease Development and Prevention

Characterization of a triad of genes in cyanophage S-2L sufficient to replace adenine by 2-aminoadenine in bacterial DNA

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