Genetic Characterization of Porcine Circovirus Type 2 from Pigs with Porcine Circovirus Associated Diseases in Argentina

Pereda, Ariel; Piñeyro, Pablo; Bratanich, Ana; Quiroga, María Alejandra; Bucafusco, Danilo; Craig, María Isabel; Cappuccio, Javier Alejandro; Machuca, Mariana Alejandra; Rimondi, Agustina; Dibárbora, Marina; Sanguinetti, Héctor Ramón; Perfumo, Carlos Juan

doi:10.5402/2011/560905

Cited by 9 publications

(4 citation statements)

References 22 publications

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“…In South America, PMWS and PCVAD have been reported; phylogenetic studies have only been conducted in Brazil, Argentina, Uruguay and Colombia [ 10 , 13 , 14 ]. The objective of this study is to evaluate the genetic diversity of PCV2 using sequences in pig affected by PMWS obtained from intensive swine farms in Chile.…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination

Neira

Ramos

Tapia

et al. 2017

Virol J

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BackgroundPorcine circovirus type 2 (PCV2) is a very small, non-enveloped and icosahedral virus, with circular single stranded DNA genome. This virus is the most ubiquitous and persistent pathogen currently affecting the swine industry worldwide. PCV2 has been implicated as the major causative agent of postweaning multisystemic wasting syndrome (PMWS), a disease which is characterized by severe immunosuppressive effects in the porcine host. Worldwide PCV2 isolates have been classified into four different genotypes, PCV2a, PCV2b, PCV2c and PCVd. The goal of this work was to conduct the first phylogenetic analysis of PCV2 in Chile.MethodsPCV2 partial ORF2 sequences (462 nt) obtained from 29 clinical cases of PMWS in 22 Chilean intensive swine farms, covering over the 90% of the local pork-production, were analyzed.Results14% and 52% of sequences belonged to the genotypes PCV2a and PCV2b, respectively. Surprisingly, 34% of sequences were PCV2a/PCV2d recombinant viruses.ConclusionsOur findings suggested that a novel cluster of Chilean sequences emerged resulting from intergenotypic recombination between PCV2a and PCV2d.

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Section: Introductionmentioning

confidence: 99%

Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination

Neira

Ramos

Tapia

et al. 2017

Virol J

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“…This virus is divided into two genotypes, a and b (Cheung et al, 2007), each of which produces a protein from the third ORF that has reported apoptotic capacity (Kiupel et al, 2005;Liu et al, 2005). Studies suggest that the PCV2b isoform is more pathogenic (Gagnon et al, 2007), and other isoforms are also being discovered with varying degrees of pathological severity (Pereda et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Transformed cell-specific induction of apoptosis by porcine circovirus type 1 viral protein 3

Hough

Rogers

Zelic

et al. 2015

Journal of General Virology

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Several members of the family Circoviridae have been shown to encode proteins with apoptotic activity. For example, both porcine circovirus type 2 (PCV2) and chicken anemia virus (CAV) encode a third viral protein (VP3) that has been shown to be cytotoxic. Interestingly, in the case of the CAV protein (designated apoptin), apoptosis is specific to transformed cell types. Similarities in genome structure and organization suggest that PCV type 1 (PCV1) may also contain a third ORF, which codes for a protein with homologous activity. To investigate this, ORF prediction followed by gene expression analyses were conducted on a gene found to be homologous to CAV and PCV2 VP3. Our data presented herein elucidate a putative ORF3 that codes for a viral protein with functional similarity to that of apoptin and PCV2 VP3. Unlike its homologues, sequence analysis revealed a highly hydrophobic, extended C-terminal domain in PCV1 VP3, which harbours a strong nuclear export signal. Subcellular localization analysis demonstrated divergent PCV1 VP3 localization patterns compared with that of CAV VP3. Interestingly, cytotoxicity studies revealed evidence that apoptosis may be selective to transformed cell types, similar to apoptin; however, PCV1 VP3 induced a dramatic G1 cell cycle arrest as opposed to the G2/M arrest observed with apoptin. These results indicate that nuclear localization of PCV1 VP3 is necessary neither for induction of apoptosis nor for transformed cell selectivity, and suggest a mechanism of action distinct from that of apoptin.

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“…5 † The presence of PCV2 was determined as previously described. 6 ‡ The presence of Ap was determined as previously described. 7 § The presence of Pm was determined as previously described.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of pig pneumonia at slaughter using polymerase chain reaction and histopathology in Argentina

Cappuccio,

Dibarbora,

Bessone

et al. 2018

JSHAP

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Genetic Characterization of Porcine Circovirus Type 2 from Pigs with Porcine Circovirus Associated Diseases in Argentina

Cited by 9 publications

References 22 publications

Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination

Genetic analysis of porcine circovirus type 2 from pigs affected with PMWS in Chile reveals intergenotypic recombination

Transformed cell-specific induction of apoptosis by porcine circovirus type 1 viral protein 3

Evaluation of pig pneumonia at slaughter using polymerase chain reaction and histopathology in Argentina

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