Genetic characterization of swine influenza viruses isolated in Japan between 2009 and 2012

Matsuu, Aya; Uchida, Yuko; Takemae, Nobuhiro; Mawatari, Takahiro; Yoneyama, Shuji; Kasai, Tomoe; Nakamura, Ryôkô; Eto, Mariko; Saito, Takehiko

doi:10.1111/j.1348-0421.2012.00501.x

Cited by 21 publications

(13 citation statements)

References 33 publications

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“…The results of serologic examination (Goto et al., ) and of virus isolation from swabs taken at slaughterhouses (T. Saito, S. Kasuo, Y. Yamashita, K. Goto, H. Kubo, unpublished data) confirmed that H3N2 viruses of human lineage have occasionally entered Japanese pig populations. After the emergence of the H1N1pdm09 virus in humans, the virus infected pig populations in Japan and reassorted with H1N2 IAV (Matsuu et al., ).…”

Section: Influenza Viruses Identified In Asian Swine Populationsmentioning

confidence: 99%

Review of Influenza A Virus in Swine Worldwide: A Call for Increased Surveillance and Research

Vincent¹,

Awada

Brown

et al. 2013

Zoonoses and Public Health

252

223

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Pigs and humans have shared influenza A viruses (IAV) since at least 1918, and many interspecies transmission events have been documented since that time. However, despite this interplay, relatively little is known regarding IAV circulating in swine around the world compared with the avian and human knowledge base. This gap in knowledge impedes our understanding of how viruses adapted to swine or man impacts the ecology and evolution of IAV as a whole and the true impact of swine IAV on human health. The pandemic H1N1 that emerged in 2009 underscored the need for greater surveillance and sharing of data on IAV in swine. In this paper, we review the current state of IAV in swine around the world, highlight the collaboration between international organizations and a network of laboratories engaged in human and animal IAV surveillance and research, and emphasize the need to increase information in high-priority regions. The need for global integration and rapid sharing of data and resources to fight IAV in swine and other animal species is apparent, but this effort requires grassroots support from governments, practicing veterinarians and the swine industry and, ultimately, requires significant increases in funding and infrastructure.

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Section: Influenza Viruses Identified In Asian Swine Populationsmentioning

confidence: 99%

Review of Influenza A Virus in Swine Worldwide: A Call for Increased Surveillance and Research

Vincent¹,

Awada

Brown

et al. 2013

Zoonoses and Public Health

252

223

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“…It is, therefore, necessary to isolate and characterize SIVs circulating in each region in a timely manner to understand the global status of SIVs. In Japan, SIVs belonging to the A(H1N1)pdm09, classical swine H1N2 virus and human seasonal virus‐derived H3N2 virus lineages have been isolated from 2009 to 2013 (Matsuu et al., ; Ozawa et al., ). Here, we genetically characterized 24 SIV strains isolated in Japan together with recent global SIV and human isolates.…”

Section: Introductionmentioning

confidence: 99%

Distribution of gene segments of the pandemic A(H1N1) 2009 virus lineage in pig populations

Okuya

Matsuu

Kawabata

et al. 2018

Transbound Emerg Dis

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Swine influenza viruses (SIVs) are important not only for pig farming, but also for public health. In fact, pandemic A(H1N1) 2009 viruses [A(H1N1)pdm09] were derived from SIVs. Therefore, timely characterization of locally circulating SIVs is necessary for understanding the global status of SIVs. To genetically characterize SIVs circulating in Japanese pig populations, we isolated 24 SIVs of three subtypes (17 H1N1, four H1N2 and three H3N2 strains) from 14 pig farms in Japan from 2013 to 2016. Genetic analyses revealed that the haemagglutinin (HA) and neuraminidase (NA) genes of the 17 H1N1 and the HA gene of one H1N2, A/swine/Aichi/02/2016 (H1N2), SIVs belonged to the A(H1N1)pdm09 lineage. More importantly, all of the remaining six gene segments (i.e., PB1, PB1, PA, NP, M and NS) of the 24 SIVs, regardless of the HA and NA subtype, were also classified as belonging to the A(H1N1)pdm09 lineage. These results indicate that gene segments of A(H1N1)pdm09 lineage are widely distributed in SIVs circulating in Japanese pig populations In addition, the NA gene of A/swine/Aichi/02/2016 (H1N2) shared less than 88.5% nucleotide identity with that of the closest relative A/swine/Miyagi/5/2003 (H1N2), which was isolated in Japan in 2003. These results indicate the sustained circulation of classical H1N2-derived SIVs with remarkable diversity in the NA genes in Japanese pig populations. These findings highlight the necessity of both intensive biosecurity systems and active SIV surveillance in pig populations worldwide for both animal and public health.

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“…However, genetically and antigenically distinct virus lineages have been individually established in North America, Asia, and Europe (11) likely due to the limited intercontinental transport of pigs, unlike those of humans or wild aquatic birds. In Japan, SIVs of A(H1N1)pdm09 virus-derived H1N1, the so-called "classical swine virus lineagederived H1N2" and North American "triple reassortant" H3N2 subtypes have been isolated recently (12), although the last H3N2 virus was isolated from a pig imported from Canada during animal quarantine and is not considered a circulating strain in Japan. Thus, SIV surveillance should be conducted in all regions and on a regular basis.…”

mentioning

confidence: 99%

Efficient Isolation of Swine Influenza Viruses by Age-Targeted Specimen Collection

et al. 2015

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hThe control of swine influenza virus (SIV) infection is paramount for increasing the productivity of pig farming and minimizing the threat of pandemic outbreaks. Thus, SIV surveillance should be conducted by region and on a regular basis. Here, we established a microneutralization assay specific for SIV seroprevalence surveillance by using reporter gene-expressing recombinant influenza viruses. Growth-based SIV seroprevalence revealed that most sows and piglets were positive for neutralizing antibodies against influenza viruses. In contrast, the 90-day-old growing pigs exhibited limited neutralizing activity in their sera, suggesting that this particular age of population is most susceptible to SIV infection and thus is an ideal age group for SIV isolation. From nasal swab specimens of healthy pigs in this age population, we were able to isolate SIVs at a higher incidence (5.3%) than those of previous reports. Nucleotide sequencing and phylogenetic analysis of the hemagglutinin (HA) genes revealed that the isolated SIVs have circulated and evolved in pigs but not have been recently introduced from humans, implying that a large number of SIV lineages may remain "undiscovered" in the global porcine populations. We propose that the 90-day-old growing pig-targeted nasal swab collection presented in this study facilitates global SIV surveillance and contributes to the detection and control of SIV infection.

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Genetic characterization of swine influenza viruses isolated in Japan between 2009 and 2012

Abstract: Eleven swine influenza viruses (SIVs) isolated from pigs in

Cited by 21 publications

References 33 publications

Review of Influenza A Virus in Swine Worldwide: A Call for Increased Surveillance and Research

Review of Influenza A Virus in Swine Worldwide: A Call for Increased Surveillance and Research

Distribution of gene segments of the pandemic A(H1N1) 2009 virus lineage in pig populations

Efficient Isolation of Swine Influenza Viruses by Age-Targeted Specimen Collection

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