Genetic characterization of the complete genome of a mutant canine parvovirus isolated in China

Li, Chuan‐Feng; Tang, Jingyu; Chen, Zongyan; Li, Qi; Huang, Zhenhua; Wang, Quan; Chen, Meng; Wang, Yong; Liu, Guangqing

doi:10.1007/s00705-017-3586-8

Cited by 9 publications

(8 citation statements)

References 24 publications

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“…Moreover, most of the current new CPV-2a had alanine at amino acid position 440; however, most of the new CPV-2b still had threonine. Amino acid substitution at position 440 (Thr-Ala) was also reported in the previous studies in China [19], India [21,22], Nigeria [24,31], Pakistan [32], South Africa [33], South Korea [25], and Uruguay [26]. Amino acid position 440 is also important because it is located at the top of the three-fold spike, the main antigenic site of the virus [34,35].…”

Section: Discussionmentioning

confidence: 53%

“…The amino acid substitution at residue 324 (Tyr-Ile) was also reported in Brazil [18], China [19], Hungary [20], India [21,22], Italy [23], Nigeria [24], South Korea [25], Taiwan [1,15], and Uruguay [26]. According to the above, amino acid position 324 has been shown to undergo a strong positive selection in the parvovirus of all carnivores [27].…”

Section: Discussionmentioning

confidence: 68%

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Dynamic evolution of canine parvovirus in Thailand

et al. 2020

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Background and Aim: According to the previous study, the circulating canine parvovirus (CPV) in Thailand is 2a and 2b. Nowadays, CPV mutants, including CPV-2c, have been identified in many parts of the world. This study aimed to investigate the genetic diversity of the circulating CPV in Thailand. Materials and Methods: Eighty-five CPV-positive fecal samples were obtained from dogs with either acute hemorrhagic diarrhea or diarrhea. The complete VP2 gene of these samples was amplified using VP2 specific primers and polymerase chain reaction (PCR). The obtained full-length VP2 sequences were analyzed and a phylogenetic tree was constructed. Results: Sixty and 25 CPV-positive fecal samples were collected in 2010 and 2018, respectively. Thirty-four samples were new CPV-2a and 31 samples were new CPV-2b due to amino acids substitution at position 297 (Ser-Ala). In 2018, 5 new CPV-2a, 19 CPV-2c, and 1 feline panleukopenia virus (FPV) were found, but no new CPV-2b was detected. Moreover, most of the CPV in this study had amino acids mutations at positions 324 and 440. The phylogenetic construction demonstrated the close relationship between the current new CPV-2a with the previous CPV-2a reported from Thailand, China, Uruguay, Vietnam, Singapore, and India. Interestingly, the current new CPV-2b in this study was not closely related to the previous CPV-2b reported in Thailand. The CPV-2c in this study was closer to Asian CPV-2c and further from either European or South America CPV-2c. Interestingly, FPV was identified in a diarrhea dog. Conclusion: The evolution of CPV in Thailand is very dynamic. Thus, it is important to monitor for CPV mutants and especially the clinical signs relating to these mutants to conduct surveillance for the emergence of new highly pathogenic CPV in the future.

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Section: Discussionmentioning

confidence: 53%

Section: Discussionmentioning

confidence: 68%

Dynamic evolution of canine parvovirus in Thailand

et al. 2020

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“…After its emergence, the original type CPV‐2 was replaced by three antigenic variants termed CPV‐2a, CPV‐2b and CPV‐2c (Buonavoglia et al, ; Parrish et al, ; Parrish, O’Connell, Evermann, & Carmichael, ). During the years, several amino acid (aa) changes were accounted in the VP2 gene sequence (Battilani et al, ; Geng et al, ; Jeoung, Ahn, & Kim, ; Nakamura et al, ; Truyen, ) and, only recently, the analysis of the NS1 gene sequence was included in the CPV phylogenies (Canuti, Rodrigues, Whitney, & Lang, ; Grecco et al, ; Han et al, ; Li et al, ; Mira et al, ; Pérez et al, ; Zhuang et al, ).…”

Section: Introductionmentioning

confidence: 99%

Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy

Mira

Purpari

Colaianni

et al. 2019

Transbound Emerg Dis

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Canine parvovirus type 2 (CPV‐2) emerged as dog pathogen in the late 1970s, causing severe and often fatal epizootics of gastroenteritis in the canine population worldwide. Although to date CPV‐2 is circulating in all continents, most of the current studies have analysed the amino acid changes accounted in the VP2 gene sequence, with limited information on virus introductions from other countries. The aim of this study was to analyse the genetic features of CPV‐2c strains currently spreading in Italy. Swabs and tissue samples were collected from dogs suspected of CPV infection. The nearly complete genome sequence from the CPV‐positive samples was obtained. The co‐circulation of two different but related CPV‐2c strains, with amino acid changes characteristic of CPV strains of Asian origin (NS1: 60V, 544F, 545F, 630P – NS2: 60V, 151N, 152V ‐ VP2: 5A/G, 267Y, 297A, 324I, 370R), were observed. The phylogenetic analyses inferred from the NS1 and VP2 gene sequences confirmed the relationship with Asian CPV‐2c strains. This study reports the spread of novel CPV‐2c mutants in Italy and supports further studies to evaluate the coexistence of genetically divergent CPV strains in the same geographical environment.

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“…Loop 1, Loop 2, and Loop 4 not only constitute the spike of the triple folding ( 24 ) but also form the antigen A site with four key amino acid sites (93, 222, 224, and 426). Residue 440 is located on the top of the 3-fold spike as a main viral genetic site, which might result in the emergence of further genetic variants ( 25 ). Loop 3 not only forms the shoulder of the triple folding but also participates in the formation of the antigen B site, with the key amino acid sites 299, 300, and 302 ( 26 ).…”

Section: Discussionmentioning

confidence: 99%

Characterization of the VP2 and NS1 genes from canine parvovirus type 2 (CPV-2) and feline panleukopenia virus (FPV) in Northern China

Chen

Hao

et al. 2022

Front. Vet. Sci.

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Canine parvovirus type 2 (CPV-2) and feline panleukopenia virus (FPV) cause severe disease in young animals, pups, and kittens. CPV-2 evolved from FPV by altering the species-specific binding of the viral capsid to the host receptor, i.e., the transferrin receptor (TfR), and CPV-2 genetic variants have been identified by specific VP2 amino acid residues (297, 426). Early studies focused on the main capsid protein VP2; however, there have been limited studies on the non-structural protein NS1. In this study, we identified the genetic variants of clinical samples in dogs and cats in northern China during 2019–2020. The genetic characterization and phylogenetic analyses of VP2 and NS1 gene were also conducted. The results revealed that the CPV-2c was identified as the major genetic variant. One new CPV-2b and two CPV-2c strains were collected from cats. Four mutation sites (60, 630, 443, and 545 amino acid residues) were located in the functional domains of the NS1 protein. The phylogenetic analysis of VP2 and NS1 genes showed that they were clustered by geographical regions and genotypes. The gene mutation rate of CPV-2 was increasing in recent years, resulting in a complex pattern of gene evolution in terms of host preference, geographical selection, and new genetic variants. This study emphasizes that continuous molecular epidemiological surveillance is required to understand the genetic diversity of FPV and CPV-2 strains.

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Genetic characterization of the complete genome of a mutant canine parvovirus isolated in China

Cited by 9 publications

References 24 publications

Dynamic evolution of canine parvovirus in Thailand

Dynamic evolution of canine parvovirus in Thailand

Spreading of canine parvovirus type 2c mutants of Asian origin in southern Italy

Characterization of the VP2 and NS1 genes from canine parvovirus type 2 (CPV-2) and feline panleukopenia virus (FPV) in Northern China

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