Genetic characterization of the β-glucuronidase enzyme from a human intestinal bacterium, Ruminococcus gnavus

Beaud, Diane; Tailliez, Patrick; Anba‐Mondoloni, Jamila

doi:10.1099/mic.0.27712-0

Cited by 137 publications

(110 citation statements)

References 32 publications

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“…The bacterial strains used in this study were E. coli TG1 (supE thiD (lac-proAB) hsdD5 F + traD36 proAB lacIZDM15) and E. coli L91 (the TG1 strain with DuidA : : Km R ) (Beaud et al, 2005). The TG1 strain, which has an active b-glucuronidase, was used to produce an isogenic derivative strain inactivated for this enzymic activity (named L91).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of mucositis induced by irinotecan after microbial colonization in germ-free mice

et al. 2015

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Mucositis is one of the most debilitating side effects of chemotherapy and some previous studies suggest a role for indigenous microbiota in the course of this pathology. Therefore, the aim of our study was to evaluate the differences in phenotype between germ-free (GF) and conventional (CV) mice, and the role of b-glucuronidase-producing bacteria in the development of irinotecan treatment in a murine model. After mucositis induction, CV mice showed a significant increase in all inflammatory parameters when compared to GF mice. CV animals also showed more lesions of the intestinal epithelium, coherent with their higher intestinal permeability. The conventionalization of GF animals reversed their phenotype to that found in CV mice. In addition, gnotobiotic mice monoassociated with an Escherichia coli strain producing b-glucuronidase showed an increased permeability when compared to gnotobiotic mice monoassociated with an E. coli strain deleted for the gene encoding b-glucuronidase, but these did not show any differences in the influx of neutrophils, eosinophils or histological characteristics. Our data confirmed that components of the gut microbiota are involved in the signs of mucositis. Nevertheless, other mechanisms than this enzyme are involved in the irinotecan treatment, since the monoassociation was not able to restore the entire phenotype observed in the CV animals with irinotecan treatment in our murine model.

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Section: Methodsmentioning

confidence: 99%

Evaluation of mucositis induced by irinotecan after microbial colonization in germ-free mice

et al. 2015

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“…Most of this process is done by liver. These exogenous compounds are poorly reabsorbed into the bloodstream and are efficiently eliminated from the body unless they are hydrolyzed [1]. ␤-Glucuronidase (GUS) enzyme is of interest that hydrolyses those biliary glucuronides.…”

Section: Introductionmentioning

confidence: 99%

Pistacia terebinthus extract: As a potential antioxidant, antimicrobial and possible β-glucuronidase inhibitor

Kavak

Altıok

Bayraktar

et al. 2010

Journal of Molecular Catalysis B: Enzymatic

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a b s t r a c tIn this study, inhibition of Escherichia coli glucuronidase (GUS) by a plant extract of Pistacia terebinthus was investigated. P. terebinthus (from Karaburun region in Turkey) leaf extract exhibits various bioactivities, such as antioxidant, antimicrobial and cytotoxic property because of its flavonoid, phenolic and alkaloid contents. HPLC analyses were performed to evaluate the composition of the extract and main flavonoid compounds such as luteolin, luteolin-7-glucoside and apigenin-7-glucoside were detected in extract. GUS activity and kinetic data of the GUS were obtained by measuring rate of production of resulting fluorescent 4-methylumbelliferone (4-MU) and phenolphthalein fluorometrically and spectrophotometrically, respectively. Plant leaves showed higher antioxidant capacity as 85 TEAC (trolox equivalent antioxidant capacity) value and higher antimicrobial activity towards Staphylococcus aureus. 92.4% GUS enzyme inhibition was achieved with 8.3 g ml −1 crude extract concentration, where IC 50 value was found as 2.1 (±0.2) g ml −1 . Kinetic studies by nonlinear regression revealed that inhibition type caused by plant extract was mixed type (noncompetitive and competitive together). K m and V max values were 96.94 ± 9.68 nM and 7.81 ± 0.16 nmol min −1 mg −1 , respectively. Inhibition constant (˛K i ) was found as 7.00 ± 0.93 nM having˛value 2.17 ± 0.93. Our results may provide useful information regarding the inhibition of microbial GUS activity, preventing deglucuronidation and reducing possible cancer risk by bioactive P. terebinthus crude extract.

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“…The enzyme activity is important for the selection of probiotic strain, because microorganisms can produce carcinogenic enzyme such as β-glucuronidase (Beaud et al, 2005). L. plantarum K255 did not produce the β-glucuronidase.…”

Section: Enzyme Activitymentioning

confidence: 99%

Physiological Characteristics and GABA Production of Lactobacillus plantarum K255 Isolated from Kimchi

Park¹,

Kim²,

Lee³

et al. 2013

Korean Journal for Food Science of Animal Resources

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As a major inhibitory neurotransmitter of the central nervous system in animals, γ-aminobutyric acid (GABA) has several physiological functions, such as anti-hypertensive, diuretic, tranquilizer and anti-stress effects in human. In order to determine strains with high GABA producing ability and glutamate decarboxylase (GAD) activity, 273 bacteria were isolated from various types of Kimchi. Strain K255 contained 386.37 µg/mL of GABA in MRS broth containing 1% MSG, 600.63 µg/mL of GABA in MRS broth containing 2% MSG and 821.24 µg/mL of GABA in MRS broth containing 3% MSG. It showed that K255 had the highest GABA production ability compared to other commercial lactic acid bacteria. K255 was identified as Lactobacillus plantarum based on its API carbohydrate fermentation pattern and 16S rDNA sequence. K255 was investigated for its physiological characteristics. The optimum growth temperature of K255 was 37 o C and cultures took 13 h to reach the pH 4.4. K255 showed more sensitive to bacitracin in a comparison of fifteen different antibiotics, and showed most resistance to kanamycin and vancomycin. Moreover, it was comparatively tolerant to bile juice and acid and displayed resistance to Escherichia coli, Salmonella Typhimurium, Staphylococcus aureus, with rates of 30.8%, 29.7%, and 23.4% respectively. These results demonstrate that K255 could be an excellent strain for the production of functional products.

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Genetic characterization of the β-glucuronidase enzyme from a human intestinal bacterium, Ruminococcus gnavus

Cited by 137 publications

References 32 publications

Evaluation of mucositis induced by irinotecan after microbial colonization in germ-free mice

Evaluation of mucositis induced by irinotecan after microbial colonization in germ-free mice

Pistacia terebinthus extract: As a potential antioxidant, antimicrobial and possible β-glucuronidase inhibitor

Physiological Characteristics and GABA Production of Lactobacillus plantarum K255 Isolated from Kimchi

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