Genetic Characterization of two Eragrostis Species using AFLP and Morphological Traits

Ayele, Mulu; Tefera, Hailu; Assefa, Kebebew; Nguyen, Henry T.

doi:10.1111/j.1601-5223.1999.00033.x

Cited by 35 publications

(22 citation statements)

References 16 publications

(12 reference statements)

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“…Methods of multivariate analysis have also been used to study tef germplasm (C ostanza et al 1979; B ekele and L ester 1981; B ekele 1986, 1996; B ekele et al 1995; A ssefa et al 1999, 2000, 2001a,b) in various aspects. On the other hand, recent studies using DNA‐based markers have shown low polymorphism in tef (B ai et al 1999, 2000; A yele et al 1999; A yele and N guyen 2000).…”

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confidence: 98%

Multivariate analysis of diversity of tef (Eragrostis tef (Zucc.) Trotter) germplasm from western and southern Ethiopia

2003

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Sixty tef germplasm populations consisting of 3000 panicle-derived lines from six western and southern regions of Ethiopia were evaluated for 17 pheno-morphic and agronomic traits on pellic Vertisols at Debre Zeit Agricultural Research Center during the 1999 main season. The objectives were to study the extent and pattern of variation of the germplasm with respect to regions and altitude zones, to classify the populations into relatively homogenous groups and to identify the major traits contributing to the overall diversity of the populations. At 75 % similarity level, the 60 populations aggregated into nine complexes of two to 10, with 12 populations remaining un-grouped. Five principal components (PC) extracted about 81 % of the gross variance among the populations. About 40 % of the variance accounted for by the first PC alone resulted largely from variations in diameters of the two basal culm internodes, grain yield and number of spikelets/panicle, shoot phytomass and grain yield/plant, and number of culm internodes. The entire regional as well as the clinal (altitude zone) variation was explained by five and two PCs, respectively. The discriminant analyses depicted about 77 % correct grouping of the 47 populations into nine clusters and about 62 % and 68 % correct origin-based classification of the germplasm in terms of altitude zones and regions, respectively. In general, the study demonstrated the existence of regional and clinal (altitude zone) variation patterns in the tef germplasm populations. The broad trait variation in the germplasm implies ample opportunities for genetic improvement of tef through selection and hybridization.

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confidence: 98%

Multivariate analysis of diversity of tef (Eragrostis tef (Zucc.) Trotter) germplasm from western and southern Ethiopia

2003

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“…According to the authors, the high GS estimates from previous studies (Ayele et al, 1999; Bai et al, 1999a, 2000; Ayele and Nguyen, 2000) using the same plant material (landraces), was a marker dependent issue rather than due to low polymorphism in tef as previously suggested. An SSR marker used to construct a phylogenetic tree for 16 natural accessions and four improved varieties of tef showed the relationship among these genotypes (Cannarozzi et al, 2014).…”

Section: Molecular Diversity In Tefmentioning

confidence: 81%

Genetic diversity in tef [Eragrostis tef (Zucc.) Trotter]

et al. 2015

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Tef [Eragrostis tef (Zucc.) Trotter] is a cereal crop resilient to adverse climatic and soil conditions, and possessing desirable storage properties. Although tef provides high quality food and grows under marginal conditions unsuitable for other cereals, it is considered to be an orphan crop because it has benefited little from genetic improvement. Hence, unlike other cereals such as maize and wheat, the productivity of tef is extremely low. In spite of the low productivity, tef is widely cultivated by over six million small-scale farmers in Ethiopia where it is annually grown on more than three million hectares of land, accounting for over 30% of the total cereal acreage. Tef, a tetraploid with 40 chromosomes (2n = 4x = 40), belongs to the family Poaceae and, together with finger millet (Eleusine coracana Gaerth.), to the subfamily Chloridoideae. It was originated and domesticated in Ethiopia. There are about 350 Eragrostis species of which E. tef is the only species cultivated for human consumption. At the present time, the gene bank in Ethiopia holds over five thousand tef accessions collected from geographical regions diverse in terms of climate and elevation. These germplasm accessions appear to have huge variability with regard to key agronomic and nutritional traits. In order to properly utilize the variability in developing new tef cultivars, various techniques have been implemented to catalog the extent and unravel the patterns of genetic diversity. In this review, we show some recent initiatives investigating the diversity of tef using genomics, transcriptomics and proteomics and discuss the prospect of these efforts in providing molecular resources that can aid modern tef breeding.

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“…Among them, amplified fragment length polymorphism (AFLP) (Vos et al, 1995) has been found to be the most appropriate in many cases. AFLPs were described as a powerful and efficient approach in population genetics and diversity analysis, molecular taxonomic classification, gene mapping and marker-assisted breeding for various crops (Ayele et al, 1999;Carr et al, 2003;Uptmoor et al, 2003). AFLP analysis provides an effective means of covering large area of the genome in a single assay (Ayad et al, 1997;Milbourne et al, 1997;Zhang et al, 1999;Muminovic et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Assessment of genetic diversity among accessions of two traditional leafy vegetables (Acmella uliginosa (L.) and Justicia tenella (Nees) T.) consumed in Benin using amplified fragment length polymorphism (AFLP) markers

Adéoti¹,

Rival²,

Dansi³

et al. 2011

Afr. J. Biotechnol.

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Amplified fragment length polymorphism (AFLP) markers were used to evaluate the genetic diversity and explore the genetic relationship among accessions of Acmella uliginosa and Justicia tenella, two leaf vegetables collected from different areas in the northwest and northeast parts of Benin (West Africa). The total number of exploitable amplicons revealed with genomic DNA from A. uliginosa was 224 with an average of 50.5% polymorphic amplicons. Using DNA from J. tenella, we obtained 34% of polymorphic amplicons from a total of 418. The coefficient of dissimilarity varied from 0.01 to 0.67 and from 0.17 to 0.62 for Acmella and Justicia, respectively. Low genetic diversity was observed among Acmella accessions although three distinct clusters could be differentiated. Contrarily, a great genetic diversity was observed among J. tenella accessions. In addition to this, most of the clusters were heterogeneous and showed the relationship between accessions collected from northeast and northwest. Our results confirm the robustness of AFLP techniques for genetic diversity studies and they provide the first set of molecular data for these two species.

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Genetic Characterization of two Eragrostis Species using AFLP and Morphological Traits

Cited by 35 publications

References 16 publications

Multivariate analysis of diversity of tef (Eragrostis tef (Zucc.) Trotter) germplasm from western and southern Ethiopia

Multivariate analysis of diversity of tef (Eragrostis tef (Zucc.) Trotter) germplasm from western and southern Ethiopia

Genetic diversity in tef [Eragrostis tef (Zucc.) Trotter]

Assessment of genetic diversity among accessions of two traditional leafy vegetables (Acmella uliginosa (L.) and Justicia tenella (Nees) T.) consumed in Benin using amplified fragment length polymorphism (AFLP) markers

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