Genetic combinations of symbionts in a vegetatively reproducing lichen,<i>Parmotrema tinctorum</i>, based on ITS rDNA sequences

Ohmura, Yoshihito; Kawachi, Masanobu; Kasai, Fumie; Watanabe, Makoto M.; Takeshita, Shunji

doi:10.1639/0007-2745(2006)109[0043:gcosia]2.0.co;2

Cited by 65 publications

(100 citation statements)

References 22 publications

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“…The interaction between mycobionts and photobionts is specific at the level of individual species, thus corroborating the results of other studies (Beck et al 2002;Piercey-Normore 2004, 2006Yahr et al 2004;Ohmura et al 2006;Hauck et al 2007). …”

Section: Random or Structured Associationssupporting

confidence: 90%

Fungal-algal interactions inRamalina menziesiiand its associated epiphytic lichen community

Werth¹

2012

The Lichenologist

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Lichens are a fascinating example of a symbiotic mutualism. It is still uncertain which processes guide fungal-photobiont interactions, and whether they are random or of a more complex nature. Here, the fungal-algal interactions in Ramalina menziesii and co-occurring taxa are analyzed by using DNA sequences of the algal Internal Transcribed Spacer region (ITS), to investigate fungalalgal associations in juvenile R. menziesii and allied species. Algal species were identified by a combination of BLAST searches, median-joining network analysis, and Bayesian phylogenetics. Fungalalgal networks were analyzed for nestedness, both at the species and haplotype level (fungal species vs. algal haplotypes), and the networks were inspected for evidence of compartmentalization. Bayesian phylogenetic trees indicated that the widespread green alga Trebouxia decolorans associated with R. menziesii, as well as six other fungal species. Four additional fungal species interacted with four different species of Trebouxia. Only in one out of ten samples were algal haplotypes shared with the nearest neighbours of juvenile R. menziesii. Fungal-algal species interactions were compartmentalized, while at the level of algal haplotypes, nestedness was found. This pattern is similar to the compartmentalization found in other intimately interacting mutualists.

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Section: Random or Structured Associationssupporting

confidence: 90%

Fungal-algal interactions inRamalina menziesiiand its associated epiphytic lichen community

Werth¹

2012

The Lichenologist

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“…This was also reported between symbionts of Parmotrema tinctorum (Ohmura et al 2006). Several explanations may provide insight into the method of dispersal for each symbiont.…”

Section: Transmission Of Algal and Fungal Symbiontssupporting

confidence: 60%

“…A, transect cluster based on the presence of genotype A; B, transect cluster based on the presence of genotype C; C, transect cluster based on the presence of genotype D; D, transect cluster based on the presence of genotype H. rDNA produced only four fungal genotypes in Parmotrema tinctorum (Delise ex Nyl.) Hale in 60 km 2 (Ohmura et al 2006). However, variation in simple sequence repeat microsatellite regions in L. pulmonaria was higher.…”

Section: Level Of Genetic Variation In the Mycobiontmentioning

confidence: 99%

Gene flow in symbionts of Cladonia arbuscula

Robertson¹,

Piercey‐Normore²

2006

The Lichenologist

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Genetic diversity in symbionts of the lichen Cladonia arbuscula was investigated. Forty-eight specimens of Cladonia arbuscula located within a 2 km range at Payuk Lake in Manitoba were collected. Population structure and method of symbiont transmission in C. arbuscula were inferred by examining the presence or absence of introns in the nuclear small subunit (SSU) ribosomal DNA (rDNA) of the mycobiont and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) rDNA in the photobiont. The mycobiont showed a high level of genetic variation and the photobiont a low level of variation within the small geographic range studied. An analysis of molecular variance of fungal genotypes showed significant population subdivision for the mycobiont when the data were analysed at P=0·05. No population subdivision was detected when P=0·001. The photobiont also showed no population subdivision. Photobiont genotypes did not correlate with mycobiont genotypes suggesting the symbionts were not transmitted together in the same propagule. Multiple fungal genotypes were also detected in C. arbuscula and possible explanations were discussed.

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“…The photobiont guild hypothesis (Ohmura et al 2006;Peksa & Skaloud 2011;Rikkinen et al 2002;Yahr et al 2006) was not supported by the findings in this study where the algal actin DNA sequences did not correspond with rock type. In contrast, photobiont preference for type of substratum has been found to occur for lichenized Asterochloris such as tree bark and rock substrata.…”

Section: Photobiont Selectivitycontrasting

confidence: 79%