Genetic compatibilities, outcrossing rates and fitness consequences across life stages of the trematode Diplostomum pseudospathaceum

Rieger, Jennifer K.; Haase, David; Reusch, Thorsten B. H.; Kalbe, Martin

doi:10.1016/j.ijpara.2013.01.005

Cited by 17 publications

(19 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, another study has shown effects of intra-specific competition in D. pseudospathaceum , resulting in reduced infection rates of clone mix treatments compared to single clone exposure [37]. In our case, the presence of outcrossed parasite genotypes in the clone mix treatment as opposed to the inbred single clones might explain the discrepancies to aforementioned study [38]. We were also not able to detect host-genotype influences on parasite-genotype infection rates.…”

Section: Discussioncontrasting

confidence: 81%

“…For experimental infection we randomly selected 12 fish per family and placed them individually in 1 l aquaria. Three individuals per family were either exposed to 100 cercaria-larvae of clone I, clone XII or a clone mix (several clonal lineages, containing inbred and outcrossed parasite genotypes) [38]. Three additional fish per family were treated the same way but not exposed to serve as control.…”

Section: Methodsmentioning

confidence: 99%

“…Three additional fish per family were treated the same way but not exposed to serve as control. All surviving infected snails were assessed for their respective parasite genotype prior to the experiment using microsatellites [38]. Only cercariae from snails where parasite genotype was unambiguous were used for the infection procedure.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Specific Gene Expression Responses to Parasite Genotypes Reveal Redundancy of Innate Immunity in Vertebrates

et al. 2014

Self Cite

View full text Add to dashboard Cite

Vertebrate innate immunity is the first line of defense against an invading pathogen and has long been assumed to be largely unspecific with respect to parasite/pathogen species. However, recent phenotypic evidence suggests that immunogenetic variation, i.e. allelic variability in genes associated with the immune system, results in host-parasite genotype-by-genotype interactions and thus specific innate immune responses. Immunogenetic variation is common in all vertebrate taxa and this reflects an effective immunological function in complex environments. However, the underlying variability in host gene expression patterns as response of innate immunity to within-species genetic diversity of macroparasites in vertebrates is unknown. We hypothesized that intra-specific variation among parasite genotypes must be reflected in host gene expression patterns. Here we used high-throughput RNA-sequencing to examine the effect of parasite genotypes on gene expression patterns of a vertebrate host, the three-spined stickleback (Gasterosteus aculeatus). By infecting naïve fish with distinct trematode genotypes of the species Diplostomum pseudospathaceum we show that gene activity of innate immunity in three-spined sticklebacks depended on the identity of an infecting macroparasite genotype. In addition to a suite of genes indicative for a general response against the trematode we also find parasite-strain specific gene expression, in particular in the complement system genes, despite similar infection rates of single clone treatments. The observed discrepancy between infection rates and gene expression indicates the presence of alternative pathways which execute similar functions. This suggests that the innate immune system can induce redundant responses specific to parasite genotypes.

show abstract

Section: Discussioncontrasting

confidence: 81%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Specific Gene Expression Responses to Parasite Genotypes Reveal Redundancy of Innate Immunity in Vertebrates

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…Experiments using individuals of single clones (selfing treatment) vs mixed clone infections (outcrossing treatment) were consistent with ID in that there was lower hatching and infection success across first and second intermediate hosts for selfed vs outcrossed treatments (see figures 1–3 in Rieger et al. ()). They reported, though, that after accounting for clonal lines in their statistical models, there was no support for ID (Rieger et al., ).…”

Section: Discussionmentioning

confidence: 59%

“…The trematode Diplostomum pseudospathaceum is also a predominately outcrossing species ( F IS = 0 across five locations in Finland; Louhi, Karvonen, Rellstab, and Jokela ()). However, a study using samples from a population in Germany was not conclusive for ID (Rieger, Haase, Reusch, & Kalbe, ). Experiments using individuals of single clones (selfing treatment) vs mixed clone infections (outcrossing treatment) were consistent with ID in that there was lower hatching and infection success across first and second intermediate hosts for selfed vs outcrossed treatments (see figures 1–3 in Rieger et al.…”

Section: Discussionmentioning

confidence: 99%

Little to no inbreeding depression in a tapeworm with mixed mating

Caballero

Criscione

2019

J of Evolutionary Biology

View full text Add to dashboard Cite

Meta‐studies on hermaphrodites have found a negative relationship between primary selfing rates and levels of inbreeding depression (ID) and, thus, generally support purging in inbred systems. However, in plants, high among‐taxa variance in ID results in no difference in the mean ID between outcrossing and mixed‐mating taxa. Selective interference likely explains high ID among mixed‐mating taxa, whereas low levels of ID among mixed‐mating taxa are not as stressed. Among animal hermaphrodites, primarily molluscs, there are little data on mixed‐mating systems. To fill a taxonomic and mating system gap, we tested for ID in a mixed‐mating tapeworm, Oochoristica javaensis. We provide a direct estimate of ID across infection of an intermediate host by comparing selfing rates at two life history stages. We found little to no evidence for ID, and the level of ID falls in line with what is reported for highly selfing species even though O. javaensis has mixed mating. We discuss this result within the context of kin mating in O. javaensis. Our results emphasize that primary selfing rates alone may be insufficient to classify the inbreeding history in all species when testing for a relationship to ID. Mixed‐mating taxa, and possibly some outcrossing taxa, may exhibit low levels of ID if biparental inbreeding is also driving purging. We advocate that ID studies report estimates of inbreeding history (e.g. FIS or identity disequilibrium) from nature‐derived adult samples to provide context rather than relying on primary selfing rates alone.

show abstract

History of microevolutionary thought in parasitology: The integration of molecular population genetics

Criscione

Janovy

Esch

2016

A Century of Parasitology

View full text Add to dashboard Cite

Genetic compatibilities, outcrossing rates and fitness consequences across life stages of the trematode Diplostomum pseudospathaceum

Cited by 17 publications

References 32 publications

Specific Gene Expression Responses to Parasite Genotypes Reveal Redundancy of Innate Immunity in Vertebrates

Specific Gene Expression Responses to Parasite Genotypes Reveal Redundancy of Innate Immunity in Vertebrates

Little to no inbreeding depression in a tapeworm with mixed mating

History of microevolutionary thought in parasitology: The integration of molecular population genetics

Contact Info

Product

Resources

About