Genetic composition and differentiation of sloe (Prunus spinosa L.) populations in Germany with respect to the tracing of reproductive plant material

Leinemann, Ludger; Kleinschmit, J.; Fussi, Barbara; Hosius, B.; Kuchma, Oleksandra; Arenhövel, Wolfgang; Lemmen, P.; Kätzel, Ralf; Rogge, Martin; Finkeldey, Reiner

doi:10.1007/s00606-014-1027-7

Cited by 17 publications

(28 citation statements)

References 35 publications

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“…The above results on genetic differentiation between the tested autochthonous populations being about 22.5% of the total genetic variation are within the range reported by other authors in autochthonous blackthorn populations in Germany and bordering Flanders (Mijnsbrugge et al 2013, Leinemann et al 2014. In this, these results differ from the 7.8% observed earlier between the seedstocks populations harvested from exactly the same authochthounous wild populations (Eimert et al 2012).…”

Section: Discussionsupporting

confidence: 86%

“…For blackthorn it seems that, at least for the regions of origin 4 and 6, the observed genetic situation does not correspond with the proposed geographic differentiation, as has been shown for other regions (Leinemann et al 2014). A more thorough analysis of populations from all regions of Germany would be required to propose corresponding and biologically meaningful regions of origin.…”

Section: Discussionmentioning

confidence: 87%

“…Interestingly, in those populations, higher differentiation of nuclear DNA was reported, although no obviuos morphometric differention was observed. Very recently, Leinemann et al (2014) analyzed nuclear and chloroplast DNA of blackthorn from 17 natural populations from across three of the proposed regions of origin and of an Italian and a Hungarian population. Here also, moderate nuclear genetic diversity was observed within the populations and the differentiation between them was similar to that observed by Mijndbrugge et al (2013) but higher than that found in seedstocks (Eimert et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Here also, moderate nuclear genetic diversity was observed within the populations and the differentiation between them was similar to that observed by Mijndbrugge et al (2013) but higher than that found in seedstocks (Eimert et al 2012). While Eimert et al (2012) and Leinemann et al (2014) analyzed genetic diversity in autochthonous seedstocks and natural populations of blackthorn in Germany, respectivelly, no direct comparison is available between the two sources. Therefore, in this paper, we analyzed the nuclear genetic diversity in natural stands of blackthorn that served as sources for seedstocks studied earlier (Eimert et al 2012) and compared the two sets.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources

Eimert¹

2015

AÇÜ Orm Fak Derg

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Genetic diversity in natural stands of autochthonous blackthorn (Prunus spinosa L.) of different German provenances has been analyzed using a highly reproducible high-annealing-temperature random amplified polymorphic DNA (HAT-RAPD) protocol. The findings were compared to those from seedstocks of the same provenances, reported earlier. Generally, genetic diversity in the natural stands was even lower (Ho 0.099-0.116) compared to the corresponding seedstocks (Ho 0.118-0.133). Furthermore, genetic differentiation was found to be moderate between natural residential sources (pairwise Fst 0.138-0.184, 22.527% variation among populations), but higher than between the seedstocks (pairwise Fst 0.086-0.104, 7.782% variation among populations). The findings are discussed in respect to German conservation law and its practical implementation.Key words: Prunus spinosa; autochthonous; RAPD; genetic diversity; differentiation Prunus Spinosa L.'nin Genetik Çeşitliliği ve Yerli Popülasyon Kaynaklarının Kullanımındaki Zorluklar ÖZET Almanya'nın farklı bölgelerindeki çakal eriği (Prunus spinosa L.) doğal türünün genetik çeşitliliği, yüksek yapışma sıcaklığında rastgele çoğaltılmış polimorfik DNA (HAT-RAPD) protokolü kullanılarak analiz edilmiştir. Bulgular daha önceki çalışmalarda kaydedilen benzer bölgelerden toplanmış tohum stoklarından elde edilen örneklerin bulgularıyla karşılaştırılmıştır. Genellikle doğal türlerdeki (suş) genetik çeşitlilik önceki çalışmalarda ölçülen değerden (Ho: 0,113) düşük olduğu (Ho; 0,116) belirlenmiştir. Dahası genetik farklılaşma doğal türlerde orta düzeyde (Fst; 0,138-0,184; populasyonlar arası varyasyon oranı % 22,527) ama tohum stoklarından elde edilen örneklerin değerlerinden (Fst; 0,086-0,104; populasyonlar arası varyasyon oranı % 7,782) daha yüksek olduğu belirlenmiştir. Bulgular Almanya koruma kanunları ve uygulamaları bakımından tartışılmıştır.

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Section: Discussionsupporting

confidence: 86%

Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources

Eimert¹

2015

AÇÜ Orm Fak Derg

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“…PCR-cpRFLP and PCR-mtRFLP have been used to separate two complex species of Leonurus cardiaca (L. cardiaca and L. quinquelobatus) (Marciniuk et al, 2014). PCR-cpRFLP has been used to investigate genetic diversity among apples (Khadivi-Khub et al, 2014), to analyze the genetic composition and differentiation of Prunus spinosa L. populations (Leinemann et al, 2014), as well as to fingerprint, identify, and authenticate varieties and species (Melgarejo et al, 2009;Sarin et al, 2013) in order to infer biogeographic and evolutionary relationships, origins, and the domestication history of plants (Dane and Liu, 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic divergence between Mexican Opuntia accessions inferred by polymerase chain reaction-restriction fragment length polymorphism analysis

Samah

Valadez-Moctezuma

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Molecular methods are powerful tools in characterizing and determining relationships between plants. The aim of this study was to study genetic divergence between 103 accessions of Mexican Opuntia. To accomplish this, polymerase chain reaction (PCR)-restriction fragment length polymorphism analysis of three chloroplast intergenic spacers (atpB-rbcL, trnL-trnF, and psbA-trnH), one chloroplast gene (ycf1), two nuclear genes (ppc and PhyC), and one mitochondrial gene (cox3) was conducted. The amplified products from all the samples had very similar molecular sizes, and there were only very small differences between the undigested PCR amplicons for all regions, with the exception of ppc. We obtained 5850 bp from the seven regions, and 136 fragments were detected with eight enzymes, 37 of which (27.2%) were polymorphic. We found that 40% of the fragments from the chloroplast regions were polymorphic, 9.8% of the bands detected in the nuclear genes were polymorphic, and 20% of the bands in the mitochondrial locus were polymorphic. trnL-trnF and psbA-trnH were the most variable regions. The Nei and Li/Dice distance was very short, and ranged from 0 to 0.12; indeed, 77 of the 103 genotypes had the same genetic profile. All the xoconostle accessions (acidic fruits) were grouped together without being separated from three genotypes of prickly pear (sweet fruits). We assume that the genetic divergence between prickly pears and xoconostles is very low, and question the number of Opuntia species currently considered in Mexico.

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Simple Sequence Repeat and S-Locus Genotyping to Assist the Genetic Characterization and Breeding of Polyploid Prunus Species, P. spinosa and P. domestica subsp. insititia

Halász

Makovics-Zsohár

Szőke³

et al. 2021

Biochem Genet

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Polyploid Prunus spinosa (2n = 4 ×) and P. domestica subsp. insititia (2n = 6 ×) represent enormous genetic potential in Central Europe, which can be exploited in breeding programs. In Hungary, 16 cultivar candidates and a recognized cultivar ‘Zempléni’ were selected from wild-growing populations including ten P. spinosa, four P. domestica subsp. insititia and three P. spinosa × P. domestica hybrids (2n = 5 ×) were also created. Genotyping in eleven simple sequence repeat (SSR) loci and the multiallelic S-locus was used to characterize genetic variability and achieve a reliable identification of tested accessions. Nine SSR loci proved to be polymorphic and eight of those were highly informative (PIC values ˃ 0.7). A total of 129 SSR alleles were identified, which means 14.3 average allele number per locus and all accessions but two clones could be discriminated based on unique SSR fingerprints. A total of 23 S-RNase alleles were identified and the complete and partial S-genotype was determined for 10 and 7 accessions, respectively. The DNA sequence was determined for a total of 17 fragments representing 11 S-RNase alleles. ‘Zempléni’ was confirmed to be self-compatible carrying at least one non-functional S-RNase allele (SJ). Our results indicate that the S-allele pools of wild-growing P. spinosa and P. domestica subsp. insititia are overlapping in Hungary. Phylogenetic and principal component analyses confirmed the high level of diversity and genetic differentiation present within the analysed accessions and indicated putative ancestor–descendant relationships. Our data confirm that S-locus genotyping is suitable for diversity studies in polyploid Prunus species but non-related accessions sharing common S-alleles may distort phylogenetic inferences.

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Genetic composition and differentiation of sloe (Prunus spinosa L.) populations in Germany with respect to the tracing of reproductive plant material

Cited by 17 publications

References 35 publications

Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources

Genetic diversity in Prunus spinosa L. – challenges in the use of autochthonous sources

Genetic divergence between Mexican Opuntia accessions inferred by polymerase chain reaction-restriction fragment length polymorphism analysis

Simple Sequence Repeat and S-Locus Genotyping to Assist the Genetic Characterization and Breeding of Polyploid Prunus Species, P. spinosa and P. domestica subsp. insititia

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