Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis

Ramos, Carlos A. N.; Araújo, Flábio R.; Alves, Lêucio Câmara; Souza, Ingrid I. F.; Guedes, Daniel S.; Soares, Cleber Oliveira

doi:10.1016/j.vetpar.2012.01.020

Cited by 24 publications

(26 citation statements)

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“…Results on the genetic conservation between rap-1 sequences of B. bovis strains have also been reported elsewhere [33,34]. Nevertheless, our isolates formed a monophyletic grouping clearly distinct from that of other published B. bovis strains, and this suggested the presence of micro-heterogeneities among the rap-1 sequences within B. bovis species.…”

Section: Resultssupporting

confidence: 82%

Molecular diagnosis and phylogenetic analysis of Babesia bigemina and Babesia bovis hemoparasites from cattle in South Africa

Mtshali

2013

BMC Vet Res

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BackgroundBabesia parasites, mainly Babesia bovis and B. bigemina, are tick-borne hemoparasites inducing bovine babesiosis in cattle globally. The clinical signs of the disease include, among others, anemia, fever and hemoglobinuria. Babesiosis is known to occur in tropical and subtropical regions of the world. In this study, we aim to provide information about the occurrence and phylogenetic relationship of B. bigemina and B. bovis species in cattle from different locations in nine provinces of South Africa.A total of 430 blood samples were randomly collected from apparently healthy cattle. These samples were genetically tested for Babesia parasitic infections using nested PCR assays with species-specific primers.ResultsNested PCR assays with Group I primer sets revealed that the overall prevalence of B. bigemina and B. bovis in all bovine samples tested was 64.7% (95% CI = 60.0-69.0) and 35.1% (95% CI = 30.6-39.8), respectively. Only 117/430 (27.2%) animals had a mixed infection. The highest prevalence of 87.5% (95% CI = 77.2-93.5) for B. bigemina was recorded in the Free State province collection sites (Ficksburg, Philippolis and Botshabelo), while North West collection sites had the highest number of animals infected with B. bovis (65.5%; 95% CI = 52.7-76.4). Phylograms were inferred based on B. bigemina-specific gp45 and B. bovis-specific rap-1 nucleotide sequences obtained with Group II nested PCR primers. Phylogenetic analysis of gp45 sequences revealed significant differences in the genotypes of B. bigemina isolates investigated, including those of strains published in GenBank. On the other hand, a phylogeny based on B. bovis rap-1 sequences indicated a similar trend of clustering among the sequences of B. bovis isolates investigated in this study.ConclusionThis study demonstrates the occurrence of Babesia parasites in cattle from different provinces of South Africa. It was also noted that the situation of Babesia parasitic infection in cattle from certain areas within the surveyed provinces had either reached endemic stability or was progressing towards stability.

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Section: Resultssupporting

confidence: 82%

Molecular diagnosis and phylogenetic analysis of Babesia bigemina and Babesia bovis hemoparasites from cattle in South Africa

Mtshali

2013

BMC Vet Res

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“…When Brazilian B . bovis isolates were analyzed in a recent study, the authors found that several genes, including MSA-2c, were highly conserved and shared high sequence identity with the genes from T2Bo [18]. In the present study, however, the MSA-1 gene sequences were found to separate into three different clades.…”

Section: Discussioncontrasting

confidence: 59%

“…bovis among cattle populations in Thailand, Brazil and Ghana has been previously reported [1, 7, 18]. A recent study based on AMA-1, ACS-1, RAP-1, TRAP, pO and MSA-2c genes found that the Brazilian B .…”

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confidence: 80%

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The Genetic Diversity of Merozoite Surface Antigen 1 (MSA-1) among <i>Babesia bovis</i> Detected from Cattle Populations in Thailand, Brazil and Ghana

et al. 2013

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In the present study, we screened blood DNA samples obtained from cattle bred in Brazil (n=164) and Ghana (n=80) for Babesia bovis using a diagnostic PCR assay and found prevalences of 14.6% and 46.3%, respectively. Subsequently, the genetic diversity of B. bovis in Thailand, Brazil and Ghana was analyzed, based on the DNA sequence of merozoite surface antigen-1 (MSA-1). In Thailand, MSA-1 sequences were relatively conserved and found in a single clade of the phylogram, while Brazilian MSA-1 sequences showed high genetic diversity and were dispersed across three different clades. In contrast, the sequences from Ghanaian samples were detected in two different clades, one of which contained only a single Ghanaian sequence. The identities among the MSA-1 sequences from Thailand, Brazil and Ghana were 99.0–100%, 57.5–99.4% and 60.3–100%, respectively, while the similarities among the deduced MSA-1 amino acid sequences within the respective countries were 98.4–100%, 59.4–99.7% and 58.7–100%, respectively. These observations suggested that the genetic diversity of B. bovis based on MSA-1 sequences was higher in Brazil and Ghana than in Thailand. The current data highlight the importance of conducting extensive studies on the genetic diversity of B. bovis before designing immune control strategies in each surveyed country.

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“…Nevertheless, these observations suggest that the levels of genetic diversity in the B. bovis MSA genes are similar in the cattle and water buffaloes of Vietnam. The MSA-2c gene is diverse in Australia (Berens et al, 2005), Sri Lanka (Sivakumar et al, 2013b), the Philippines (Tattiyapong et al, 2014), and Israel (Molad et al, 2014), whereas this gene is conserved in Mongolia (Altangeral et al, 2012) and Brazil (Ramos et al, 2012). In addition, the sequences isolated in Argentina, Mexico, and USA shared high similarities, suggesting that the MSA-2c from distant geographical locations may also sometimes be conserved (Dominguez et al, 2010;Florin-Christensen et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes

Yokoyama

Sivakumar

Tuvshintulga

et al. 2015

Infection, Genetics and Evolution

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The genes that encode merozoite surface antigens (MSAs) in Babesia bovis are genetically diverse. In this study, we analyzed the genetic diversity of B. bovis MSA-1, MSA-2b, and MSA-2c genes in Vietnamese cattle and water buffaloes. Blood DNA samples from 258 cattle and 49 water buffaloes reared in the Thua Thien Hue province of Vietnam were screened with a B. bovis-specific diagnostic PCR assay. The B. bovis-positive DNA samples (23 cattle and 16 water buffaloes) were then subjected to PCR assays to amplify the MSA-1, MSA-2b, and MSA-2c genes. Sequencing analyses showed that the Vietnamese MSA-1 and MSA-2b sequences are genetically diverse, whereas MSA-2c is relatively conserved. The nucleotide identity values for these MSA gene sequences were similar in the cattle and water buffaloes. Consistent with the sequencing data, the Vietnamese MSA-1 and MSA-2b sequences were dispersed across several clades in the corresponding phylogenetic trees, whereas the MSA-2c sequences occurred in a single clade. Cattle- and water-buffalo-derived sequences also often clustered together on the phylogenetic trees. The Vietnamese MSA-1, MSA-2b, and MSA-2c sequences were then screened for recombination with automated methods. Of the seven recombination events detected, five and two were associated with the MSA-2b and MSA-2c recombinant sequences, respectively, whereas no MSA-1 recombinants were detected among the sequences analyzed. Recombination between the sequences derived from cattle and water buffaloes was very common, and the resultant recombinant sequences were found in both host animals. These data indicate that the genetic diversity of the MSA sequences does not differ between cattle and water buffaloes in Vietnam. They also suggest that recombination between the B. bovis MSA sequences in both cattle and water buffaloes might contribute to the genetic variation in these genes in Vietnam.

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Genetic conservation of potentially immunogenic proteins among Brazilian isolates of Babesia bovis

Cited by 24 publications

References 25 publications

Molecular diagnosis and phylogenetic analysis of Babesia bigemina and Babesia bovis hemoparasites from cattle in South Africa

Molecular diagnosis and phylogenetic analysis of Babesia bigemina and Babesia bovis hemoparasites from cattle in South Africa

The Genetic Diversity of Merozoite Surface Antigen 1 (MSA-1) among <i>Babesia bovis</i> Detected from Cattle Populations in Thailand, Brazil and Ghana

Genetic variations in merozoite surface antigen genes of Babesia bovis detected in Vietnamese cattle and water buffaloes

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