Genetic conspecificity of the worldwide populations of Didemnum vexillum Kott, 2002

Stefaniak, Lauren M.; Lambert, Gretchen; Gittenberger, Adriaan; Zhang, H.; Lin, Shangrong; Whitlatch, Robert B.; Locke, Andrea; Carman, Mary R.

doi:10.3391/ai.2009.4.1.3

Cited by 125 publications

(108 citation statements)

References 35 publications

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“…Total PCR reaction volume was 50 ml, including 10 ml of 5 Â Buffer, 0.4 ml of bovine serum albumin (BSA; 10 mg/ml), 0.25 ml of My Taq DNA Polymerase (Bioline, London, United Kingdom), 2 ml of each primer (10 mM) and 1 ml of template DNA. Two sets of primer pairs were used for COI amplification, the 'universal' primers LCO1490 and HCO2198 (Folmer et al, 1994) and the ascidian-specific primers Tun_forward and Tun_Reverse2 (Stefaniak et al, 2009 Figure S4). …”

Section: Dna Extractionmentioning

confidence: 99%

Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians

et al. 2013

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Ascidians are ecologically important components of marine ecosystems yet the ascidian microbiota remains largely unexplored beyond a few model species. We used 16S rRNA gene tag pyrosequencing to provide a comprehensive characterization of microbial symbionts in the tunic of 42 Great Barrier Reef ascidian samples representing 25 species. Results revealed high bacterial biodiversity (3 217 unique operational taxonomic units (OTU 0.03 ) from 19 described and 14 candidate phyla) and the widespread occurrence of ammonia-oxidizing Thaumarchaeota in coral reef ascidians (24 of 25 host species). The ascidian microbiota was clearly differentiated from seawater microbial communities and included symbiont lineages shared with other invertebrate hosts as well as unique, ascidian-specific phylotypes. Several rare seawater microbes were markedly enriched (200-700 fold) in the ascidian tunic, suggesting that the rare biosphere of seawater may act as a conduit for horizontal symbiont transfer. However, most OTUs (71%) were rare and specific to single hosts and a significant correlation between host relatedness and symbiont community similarity was detected, indicating a high degree of host-specificity and potential role of vertical transmission in structuring these communities. We hypothesize that the complex ascidian microbiota revealed herein is maintained by the dynamic microenvironments within the ascidian tunic, offering optimal conditions for different metabolic pathways such as ample chemical substrate (ammonia-rich host waste) and physical habitat (high oxygen, low irradiance) for nitrification. Thus, ascidian hosts provide unique and fertile niches for diverse microorganisms and may represent an important and previously unrecognized habitat for nitrite/nitrate regeneration in coral reef ecosystems.

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Section: Dna Extractionmentioning

confidence: 99%

Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians

et al. 2013

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“…Recently, the taxonomic issue has been alleviated using genetic markers based on global samples. Coupled with morphological evidence, D. vexillum seems to be the most appropriate name for this ascidian species (Lambert 2009;Stefaniak et al 2009). …”

Section: Invasive Ascidians and Their Invasion Historymentioning

confidence: 99%

“…More importantly, these studies call for combining morphological and genetic approaches to solving the problem of ambiguous taxonomy in ascidians. Genetic identification can strongly enhance, and in some cases would appear to have many advantages over, traditional morphology (Stefaniak et al 2009;Geller et al 2010). In addition, the use of genetic data has facilitated deep understanding of both fundamental questions, such as invasion histories, invasion sources and factors responsible for introduction and post-establishment spread (e.g., Estoup and Guillemaud 2010), and more applied topics such as development of effective management strategies and sustainable science-based policies (see review by Geller et al 2010).…”

Section: Taxonomy: a Call For Further Investigation Using Genetic Metmentioning

confidence: 99%

“…This problem mainly stems from the presence of available taxonomic keys, species complexes, and/or high geographical morphological variation (e.g., Stefaniak et al 2009;Zhan et al 2010;Bock et al 2012). Recent studies demonstrate that many invasive ascidians are actually species complexes containing morphologically cryptic but genetically distinct species (e.g., Zhan et al 2010;Bock et al 2012;Pérez-Portela et al 2013).…”

Section: Taxonomy: a Call For Further Investigation Using Genetic Metmentioning

confidence: 99%

“…For example, the first detection date of B. violaceus can be either 1945(collection made in 1939van Name 1945;unconfirmed) or the 1970s (Fay and Johnson 1971;confirmed), with confusion with Botrylloides diegensis on the west coast of North America preventing clear determination. D. vexillum poses an even more difficult problem: It has been problematic to identify this invader, to determine how many species are involved, and to clarify its invasion history, mainly owing to relatively few available diagnostic characters and high morphological variability among populations collected from different environments/areas (Lambert 2009;Stefaniak et al 2009). D. vexillum has been mis-identified as at least five native species locally, and researchers have used several different names, such as Didemnum spA, D. vexillum, and Didemnum vestum (Lambert 2009;Stefaniak et al 2009).…”

Section: Invasive Ascidians and Their Invasion Historymentioning

confidence: 99%

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Ascidians as models for studying invasion success

et al. 2015

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Gretchen Lambert: taxonomist, explorer, and historian of the ascidian community. Active: 1968‐present

Nydam

2023

Genesis

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In 1965, she moved to the University of Washington to start her Master's Degree in Zoology, which she obtained in 1967. She worked with the influential marine ecologist Robert Paine. During her time at the University of Washington, she met Charles Lambert, who became her partner in life and research. Very early on, Charles convinced her of the experimental advantages of ascidians as research animals. From 1970 to 1998, she and Charles worked at California State University, Fullerton (CSUF). Gretchen was a lecturer, a museum coordinator, a laboratory coordinator for introductory biology, a research associate and a senior research associate. She and Charles worked closely together on ascidian ecology and development.

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Genetic conspecificity of the worldwide populations of Didemnum vexillum Kott, 2002

Cited by 125 publications

References 35 publications

Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians

Down under the tunic: bacterial biodiversity hotspots and widespread ammonia-oxidizing archaea in coral reef ascidians

Ascidians as models for studying invasion success

Gretchen Lambert: taxonomist, explorer, and historian of the ascidian community. Active: 1968‐present

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